Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study
Abstract Background This animal study sought to evaluate two novel nanomaterials for pulpotomy of primary teeth and assess the short-term pulpal response and hard tissue formation in dogs. The results were compared with mineral trioxide aggregate (MTA). Methods This in vivo animal study on dogs eval...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-04098-9 |
| _version_ | 1797258957432029184 |
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| author | Sahar Talebi Nosrat Nourbakhsh Ardeshir Talebi Amir Abbas Nourbakhsh Abbas Haghighat Maziar Manshayi Hamid Reza Bakhsheshi Razieh Karimi Rahman Nazeri Kenneth J.D. Mackenzie |
| author_facet | Sahar Talebi Nosrat Nourbakhsh Ardeshir Talebi Amir Abbas Nourbakhsh Abbas Haghighat Maziar Manshayi Hamid Reza Bakhsheshi Razieh Karimi Rahman Nazeri Kenneth J.D. Mackenzie |
| author_sort | Sahar Talebi |
| collection | DOAJ |
| description | Abstract Background This animal study sought to evaluate two novel nanomaterials for pulpotomy of primary teeth and assess the short-term pulpal response and hard tissue formation in dogs. The results were compared with mineral trioxide aggregate (MTA). Methods This in vivo animal study on dogs evaluated 48 primary premolar teeth of 4 mongrel female dogs the age of 6–8 weeks, randomly divided into four groups (n = 12). The teeth underwent complete pulpotomy under general anesthesia. The pulp tissue was capped with MCM-48, MCM-48/Hydroxyapatite (HA), MTA (positive control), and gutta-percha (negative control), and the teeth were restored with intermediate restorative material (IRM) paste and amalgam. After 4–6 weeks, the teeth were extracted and histologically analyzed to assess the pulpal response to the pulpotomy agent. Results The data were analyzed using the Kruskal‒Wallis, Fisher’s exact, Spearman’s, and Mann‒Whitney tests. The four groups were not significantly different regarding the severity of inflammation (P = 0.53), extent of inflammation (P = 0.72), necrosis (P = 0.361), severity of edema (P = 0.52), extent of edema (P = 0.06), or connective tissue formation (P = 0.064). A significant correlation was noted between the severity and extent of inflammation (r = 0.954, P < 0.001). The four groups were significantly different regarding the frequency of bone formation (P = 0.012), extent of connective tissue formation (P = 0.047), severity of congestion (P = 0.02), and extent of congestion (P = 0.01). No bone formation was noted in the gutta-percha group. The type of newly formed bone was not significantly different among the three experimental groups (P = 0.320). Conclusion MCM-48 and MCM-48/HA are bioactive nanomaterials that may serve as alternatives for pulpotomy of primary teeth due to their ability to induce hard tissue formation. The MCM-48 and MCM-48/HA mesoporous silica nanomaterials have the potential to induce osteogenesis and tertiary (reparative) dentin formation. |
| first_indexed | 2024-04-24T23:01:47Z |
| format | Article |
| id | doaj.art-9c47256c830e4e0581a61ddbcd6cb440 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-24T23:01:47Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-9c47256c830e4e0581a61ddbcd6cb4402024-03-17T12:40:34ZengBMCBMC Oral Health1472-68312024-03-0124111810.1186/s12903-024-04098-9Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal studySahar Talebi0Nosrat Nourbakhsh1Ardeshir Talebi2Amir Abbas Nourbakhsh3Abbas Haghighat4Maziar Manshayi5Hamid Reza Bakhsheshi6Razieh Karimi7Rahman Nazeri8Kenneth J.D. Mackenzie9Dentist, Research Committee, School of Dentistry, Isfahan University of Medical SciencesDepartment of Pediatric Dentistry, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical SciencesDepartment of Pathology, Medical School, Dental Research Center, Dental Research Institute, Isfahan University of Medical SciencesDepartment of Materials Science, Shahreza Branch, Islamic Azad UniversityDepartment of Maxillofacial Surgery, Dental Research Center, Dental Research Institute, School of Dentistry, Isfahan University of Medical SciencesDVM. Dental Science Research Center. Dentistry faculty, Isfahan University of Medical SciencesAdvanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad UniversityAdvanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad UniversityDentist, School of Dentistry, Isfahan University of Medical SciencesMacDiarmid Institute for Advanced Materials and Nanotechnology, Victoria University of WellingtonAbstract Background This animal study sought to evaluate two novel nanomaterials for pulpotomy of primary teeth and assess the short-term pulpal response and hard tissue formation in dogs. The results were compared with mineral trioxide aggregate (MTA). Methods This in vivo animal study on dogs evaluated 48 primary premolar teeth of 4 mongrel female dogs the age of 6–8 weeks, randomly divided into four groups (n = 12). The teeth underwent complete pulpotomy under general anesthesia. The pulp tissue was capped with MCM-48, MCM-48/Hydroxyapatite (HA), MTA (positive control), and gutta-percha (negative control), and the teeth were restored with intermediate restorative material (IRM) paste and amalgam. After 4–6 weeks, the teeth were extracted and histologically analyzed to assess the pulpal response to the pulpotomy agent. Results The data were analyzed using the Kruskal‒Wallis, Fisher’s exact, Spearman’s, and Mann‒Whitney tests. The four groups were not significantly different regarding the severity of inflammation (P = 0.53), extent of inflammation (P = 0.72), necrosis (P = 0.361), severity of edema (P = 0.52), extent of edema (P = 0.06), or connective tissue formation (P = 0.064). A significant correlation was noted between the severity and extent of inflammation (r = 0.954, P < 0.001). The four groups were significantly different regarding the frequency of bone formation (P = 0.012), extent of connective tissue formation (P = 0.047), severity of congestion (P = 0.02), and extent of congestion (P = 0.01). No bone formation was noted in the gutta-percha group. The type of newly formed bone was not significantly different among the three experimental groups (P = 0.320). Conclusion MCM-48 and MCM-48/HA are bioactive nanomaterials that may serve as alternatives for pulpotomy of primary teeth due to their ability to induce hard tissue formation. The MCM-48 and MCM-48/HA mesoporous silica nanomaterials have the potential to induce osteogenesis and tertiary (reparative) dentin formation.https://doi.org/10.1186/s12903-024-04098-9PulpotomyVital pulpotomyPrimary teeth, mineral trioxide aggregateMCM-48HydroxyapatiteHard tissue formation |
| spellingShingle | Sahar Talebi Nosrat Nourbakhsh Ardeshir Talebi Amir Abbas Nourbakhsh Abbas Haghighat Maziar Manshayi Hamid Reza Bakhsheshi Razieh Karimi Rahman Nazeri Kenneth J.D. Mackenzie Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study BMC Oral Health Pulpotomy Vital pulpotomy Primary teeth, mineral trioxide aggregate MCM-48 Hydroxyapatite Hard tissue formation |
| title | Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study |
| title_full | Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study |
| title_fullStr | Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study |
| title_full_unstemmed | Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study |
| title_short | Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study |
| title_sort | hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials mcm 48 and mcm 48 hydroxyapatite an in vivo animal study |
| topic | Pulpotomy Vital pulpotomy Primary teeth, mineral trioxide aggregate MCM-48 Hydroxyapatite Hard tissue formation |
| url | https://doi.org/10.1186/s12903-024-04098-9 |
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