Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of India
Faecal samples (363) from kids, lambs, calves and buffalo calves of below 3 months of age were collected from various parts of India and screened microscopically for Cryptosporidium oocysts using modified Ziehl–Neelsen method of staining. Microscopically positive samples (20) were genotyped by PCR...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2014-08-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/43208 |
| _version_ | 1797643412904607744 |
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| author | R L RAKESH P S BANERJEE RAJAT GARG P S MAURYA K KUNDU S S JACOB O K RAINA |
| author_facet | R L RAKESH P S BANERJEE RAJAT GARG P S MAURYA K KUNDU S S JACOB O K RAINA |
| author_sort | R L RAKESH |
| collection | DOAJ |
| description |
Faecal samples (363) from kids, lambs, calves and buffalo calves of below 3 months of age were collected from various parts of India and screened microscopically for Cryptosporidium oocysts using modified Ziehl–Neelsen method of staining. Microscopically positive samples (20) were genotyped by PCR amplification of the partial 18S rRNA region and subsequent digestion by SspI, VspI and MboII restriction enzymes. Based on the PCR-RFLP patterns of 18S rRNA, all the 20 samples were found positive for C. parvum. All the positive samples were also used for amplification of partial actin gene of Cryptosporidium spp. For further confirmation of the species of Cryptosporidium, amplified 818 bp partial actin gene of 3 representative isolates was cloned and sequenced. The sequence and phylogenetic analysis of PCR-positive samples confirmed the presence of C. parvum. Thus, actin gene can also be used for specific molecular diagnosis of Cryptosporidium spp., in addition to 18S rRNA. These findings also indicated that young domestic ruminants can be a potent source of cryptosporidial infection for humans and animals in India.
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| first_indexed | 2024-03-11T14:14:24Z |
| format | Article |
| id | doaj.art-9c475ffec697438ea32f3467e198465e |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-11T14:14:24Z |
| publishDate | 2014-08-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-9c475ffec697438ea32f3467e198465e2023-11-01T11:01:43ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272014-08-0184810.56093/ijans.v84i8.43208Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of IndiaR L RAKESH0P S BANERJEE1RAJAT GARG2P S MAURYA3K KUNDU4S S JACOB5O K RAINA6Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India Faecal samples (363) from kids, lambs, calves and buffalo calves of below 3 months of age were collected from various parts of India and screened microscopically for Cryptosporidium oocysts using modified Ziehl–Neelsen method of staining. Microscopically positive samples (20) were genotyped by PCR amplification of the partial 18S rRNA region and subsequent digestion by SspI, VspI and MboII restriction enzymes. Based on the PCR-RFLP patterns of 18S rRNA, all the 20 samples were found positive for C. parvum. All the positive samples were also used for amplification of partial actin gene of Cryptosporidium spp. For further confirmation of the species of Cryptosporidium, amplified 818 bp partial actin gene of 3 representative isolates was cloned and sequenced. The sequence and phylogenetic analysis of PCR-positive samples confirmed the presence of C. parvum. Thus, actin gene can also be used for specific molecular diagnosis of Cryptosporidium spp., in addition to 18S rRNA. These findings also indicated that young domestic ruminants can be a potent source of cryptosporidial infection for humans and animals in India. https://epubs.icar.org.in/index.php/IJAnS/article/view/4320818S rRNAActin geneCryptosporidium parvumGenotypingIndia |
| spellingShingle | R L RAKESH P S BANERJEE RAJAT GARG P S MAURYA K KUNDU S S JACOB O K RAINA Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of India Indian Journal of Animal Sciences 18S rRNA Actin gene Cryptosporidium parvum Genotyping India |
| title | Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of India |
| title_full | Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of India |
| title_fullStr | Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of India |
| title_full_unstemmed | Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of India |
| title_short | Genotyping of Cryptosporidium spp. isolated from young domestic ruminants in some targeted areas of India |
| title_sort | genotyping of cryptosporidium spp isolated from young domestic ruminants in some targeted areas of india |
| topic | 18S rRNA Actin gene Cryptosporidium parvum Genotyping India |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/43208 |
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