A qualitative assessment of direct-labeled cDNA products prior to microarray analysis
<p>Abstract</p> <p>Background</p> <p>The success of the microarray process in determining differential gene expression of thousands of genes is dependent upon the quality and integrity of the starting RNA, this being particularly true of direct labeling via a reverse tr...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-03-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/6/36 |
| _version_ | 1818050660964237312 |
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| author | Lobenhofer Edward K Grissom Sherry F Tucker Charles J |
| author_facet | Lobenhofer Edward K Grissom Sherry F Tucker Charles J |
| author_sort | Lobenhofer Edward K |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The success of the microarray process in determining differential gene expression of thousands of genes is dependent upon the quality and integrity of the starting RNA, this being particularly true of direct labeling via a reverse transcription procedure. Furthermore, an RNA of reasonable quality still may not yield reliable hybridization data if the labeling efficiency was poor.</p> <p>Results</p> <p>Here we present a novel assay for assessing the quality of directly labeled fluorescent cDNA prior to microarray hybridization utilizing the Agilent 2100 Bioanalyzer, which employs microfluidic technology for the analysis of nucleic acids and proteins. Using varying amounts of RNase to simulate RNA degradation, we show the strength of this un-advertised assay in determining the relative amounts of cDNA obtained from a direct labeling reaction.</p> <p>Conclusion</p> <p>Utilization of this method in the lab will help to prevent the costly mistake of hybridizing poor quality direct labeled products to expensive arrays.</p> |
| first_indexed | 2024-12-10T10:57:01Z |
| format | Article |
| id | doaj.art-9c4c5c91cb0f4b47abf3d11e8dc60bf7 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-10T10:57:01Z |
| publishDate | 2005-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-9c4c5c91cb0f4b47abf3d11e8dc60bf72022-12-22T01:51:49ZengBMCBMC Genomics1471-21642005-03-01613610.1186/1471-2164-6-36A qualitative assessment of direct-labeled cDNA products prior to microarray analysisLobenhofer Edward KGrissom Sherry FTucker Charles J<p>Abstract</p> <p>Background</p> <p>The success of the microarray process in determining differential gene expression of thousands of genes is dependent upon the quality and integrity of the starting RNA, this being particularly true of direct labeling via a reverse transcription procedure. Furthermore, an RNA of reasonable quality still may not yield reliable hybridization data if the labeling efficiency was poor.</p> <p>Results</p> <p>Here we present a novel assay for assessing the quality of directly labeled fluorescent cDNA prior to microarray hybridization utilizing the Agilent 2100 Bioanalyzer, which employs microfluidic technology for the analysis of nucleic acids and proteins. Using varying amounts of RNase to simulate RNA degradation, we show the strength of this un-advertised assay in determining the relative amounts of cDNA obtained from a direct labeling reaction.</p> <p>Conclusion</p> <p>Utilization of this method in the lab will help to prevent the costly mistake of hybridizing poor quality direct labeled products to expensive arrays.</p>http://www.biomedcentral.com/1471-2164/6/36 |
| spellingShingle | Lobenhofer Edward K Grissom Sherry F Tucker Charles J A qualitative assessment of direct-labeled cDNA products prior to microarray analysis BMC Genomics |
| title | A qualitative assessment of direct-labeled cDNA products prior to microarray analysis |
| title_full | A qualitative assessment of direct-labeled cDNA products prior to microarray analysis |
| title_fullStr | A qualitative assessment of direct-labeled cDNA products prior to microarray analysis |
| title_full_unstemmed | A qualitative assessment of direct-labeled cDNA products prior to microarray analysis |
| title_short | A qualitative assessment of direct-labeled cDNA products prior to microarray analysis |
| title_sort | qualitative assessment of direct labeled cdna products prior to microarray analysis |
| url | http://www.biomedcentral.com/1471-2164/6/36 |
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