Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito
<p><em><strong>Latar belakang</strong>: Survei virologi pada nyamuk vektor dapat digunakan sebagai Sistem Kewaspadaan Dini untuk mencegah penularan Demam dengue di suatu daerah. Pemeriksaan laboratoris untuk deteksi virus Dengue pada nyamuk seperti isolasi virus, Polymerase C...
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| Format: | Article |
| Language: | English |
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Badan Penelitian dan Pengembangan Kesehatan
2012-07-01
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| Series: | Health Science Journal of Indonesia |
| Subjects: | |
| Online Access: | http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/88 |
| _version_ | 1828353238952312832 |
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| author | Dyah Widiastuti Bambang Yunianto Sitti Rahmah Umniyati Nastiti Wijayanti |
| author_facet | Dyah Widiastuti Bambang Yunianto Sitti Rahmah Umniyati Nastiti Wijayanti |
| author_sort | Dyah Widiastuti |
| collection | DOAJ |
| description | <p><em><strong>Latar belakang</strong>: Survei virologi pada nyamuk vektor dapat digunakan sebagai Sistem Kewaspadaan Dini untuk mencegah penularan Demam dengue di suatu daerah. Pemeriksaan laboratoris untuk deteksi virus Dengue pada nyamuk seperti isolasi virus, Polymerase Chain Reaction (PCR) dan Direct Fluorescent-Antibody (DFA) memerlukan keahlian yang tinggi, peralatan yang mahal dan waktu yang lama. Suatu metode berdasarkan imunositokimia menggunakan antibody monoclonal DSSE10 memiliki beberapa kelebihan. Tujuan penelitian ini untuk mengevaluasi sensitifitas dan spesifitas pemeriksaan imunositokimia dibandingkan metode Reverse Transcription-Polymerase Chain Reaction (RT-PCR) untuk mendeteksi infeksi Virus Dengue 3. </em></p><p><em><strong>Metode</strong>: Penelitian eksperimental dilakukan di laboratorium Parasitologi Fakultas Kedokteran Universitas Gajah Mada (UGM) pada bulan Mei 2009-Oktober 2010. Sebanyak 22 Ae. aegypti yang diinfeksi virus Dengue 3 digunakan sebagai kelompok infeksius dan 35 nyamuk yang tidak diinfeksi sebagai kelompok non infeksius. Pemeriksaan imunositokimia Streptavidin Biotin Peroxidase Complex (SBPC) menggunakan antibodi monoklonal DSSE10 dilakukan pada sediaan head squash Ae .aegypti untuk mendeteksi antigen virus Dengue 3. Pemeriksaan RT-PCR sebagai baku emas diaplikasikan pada toraks nyamuk.</em></p><p><em><strong>Hasil</strong>: Nilai Kappa menunjukkan kesepakatan yang baik antara dua orang pemeriksa (0,63). Imunositokimia mendeteksi antigen virus Dengue-3 dengan sensitivitas yang sama dengan RT-PCR (sensitivitas 100%). Namun spesifisitas IC lebih rendah dibanding RT-PCR (spesifisitas 91%) karena beberapa hasil positif palsu muncul pada pemeriksaan ini. </em></p><p><em><strong>Kesimpulan</strong>: Metode IC memiliki nilai sensitivitas dan spesifisitas yang tinggi dibandingkan dengan metode RT-PCR. Metode IC ini dapat digunakan untuk surveilans virus Dengue pada nyamuk vektor. (Health Science Indones 2011;2:87-91).</em></p><p><strong>Abstract</strong></p><p><strong>Background</strong>: Virological surveillance provides an early warning sign for the risk of transmission in an area. Laboratory tests for dengue virus infection on mosquitoes include isolation of the virus, Polymerase Chain Reaction (PCR) and Direct Fluorescent-Antibody (DFA) requires a high level of technical skill, expensive equipment, and time-consuming. A method based on immunocytochemical (IC) using monoclonal antibody DSSE10 has several advantages. This study aimed to evaluate sensitivity and specificity IC assay compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR) as gold standard to detect Dengue Virus (DENV)-3 infections in mosquito Aedes aegypti.</p><p><strong>Methods</strong>: An experimental study was conducted in laboratory of Medical Parasitology, Faculty of Medicine, Universitas Gadjah Mada (UGM) in May 2009 until October 2010. A total of 22 artificially-infected adult Ae. aegypti mosquitoes of DENV 3 were used as infectious samples and 35 non-infected adult Ae. aegypti mosquitoes were used as normal ones. The IC Streptavidin Biotin Peroxidase Complex (SBPC) assay using monoclonal antibody DSSE10 was applied in mosquito head squash to detect Dengue virus antigen. RT-PCR as a gold standard was applied in mosquito thorax.</p><p><strong>Results</strong>: The kappa value showed a good agreement between two observers (kappa value 0.63). IC could detect dengue virus antigen as sensitive as RT-PCR (sensitivity 100%). But IC was less specific than RT-PCR (specificity 91%) because some false positive results were found in this method.</p><p><strong>Conclusion</strong>: The IC method has a high sensitivity and high specificity compared with RT-PCR. This IC method may be useful for virological surveillance of dengue infected Aedes mosquitoes. (Health Science Indones 2011;2:87-91).</p><p> </p> |
| first_indexed | 2024-04-14T02:04:53Z |
| format | Article |
| id | doaj.art-9c50887efe064b0ca3553f19a6d2edb6 |
| institution | Directory Open Access Journal |
| issn | 2087-7021 2338-3437 |
| language | English |
| last_indexed | 2024-04-14T02:04:53Z |
| publishDate | 2012-07-01 |
| publisher | Badan Penelitian dan Pengembangan Kesehatan |
| record_format | Article |
| series | Health Science Journal of Indonesia |
| spelling | doaj.art-9c50887efe064b0ca3553f19a6d2edb62022-12-22T02:18:42ZengBadan Penelitian dan Pengembangan KesehatanHealth Science Journal of Indonesia2087-70212338-34372012-07-0122 Des879191Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquitoDyah Widiastuti0Bambang Yunianto1Sitti Rahmah Umniyati2Nastiti Wijayanti3Banjarnegara Vector Control Research Unit, National Institute of Health Research and DevelopmentBanjarnegara Vector Control Research Unit, National Institute of Health Research and DevelopmentDepartment of Parasitology, Faculty of Medicine, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah Mada<p><em><strong>Latar belakang</strong>: Survei virologi pada nyamuk vektor dapat digunakan sebagai Sistem Kewaspadaan Dini untuk mencegah penularan Demam dengue di suatu daerah. Pemeriksaan laboratoris untuk deteksi virus Dengue pada nyamuk seperti isolasi virus, Polymerase Chain Reaction (PCR) dan Direct Fluorescent-Antibody (DFA) memerlukan keahlian yang tinggi, peralatan yang mahal dan waktu yang lama. Suatu metode berdasarkan imunositokimia menggunakan antibody monoclonal DSSE10 memiliki beberapa kelebihan. Tujuan penelitian ini untuk mengevaluasi sensitifitas dan spesifitas pemeriksaan imunositokimia dibandingkan metode Reverse Transcription-Polymerase Chain Reaction (RT-PCR) untuk mendeteksi infeksi Virus Dengue 3. </em></p><p><em><strong>Metode</strong>: Penelitian eksperimental dilakukan di laboratorium Parasitologi Fakultas Kedokteran Universitas Gajah Mada (UGM) pada bulan Mei 2009-Oktober 2010. Sebanyak 22 Ae. aegypti yang diinfeksi virus Dengue 3 digunakan sebagai kelompok infeksius dan 35 nyamuk yang tidak diinfeksi sebagai kelompok non infeksius. Pemeriksaan imunositokimia Streptavidin Biotin Peroxidase Complex (SBPC) menggunakan antibodi monoklonal DSSE10 dilakukan pada sediaan head squash Ae .aegypti untuk mendeteksi antigen virus Dengue 3. Pemeriksaan RT-PCR sebagai baku emas diaplikasikan pada toraks nyamuk.</em></p><p><em><strong>Hasil</strong>: Nilai Kappa menunjukkan kesepakatan yang baik antara dua orang pemeriksa (0,63). Imunositokimia mendeteksi antigen virus Dengue-3 dengan sensitivitas yang sama dengan RT-PCR (sensitivitas 100%). Namun spesifisitas IC lebih rendah dibanding RT-PCR (spesifisitas 91%) karena beberapa hasil positif palsu muncul pada pemeriksaan ini. </em></p><p><em><strong>Kesimpulan</strong>: Metode IC memiliki nilai sensitivitas dan spesifisitas yang tinggi dibandingkan dengan metode RT-PCR. Metode IC ini dapat digunakan untuk surveilans virus Dengue pada nyamuk vektor. (Health Science Indones 2011;2:87-91).</em></p><p><strong>Abstract</strong></p><p><strong>Background</strong>: Virological surveillance provides an early warning sign for the risk of transmission in an area. Laboratory tests for dengue virus infection on mosquitoes include isolation of the virus, Polymerase Chain Reaction (PCR) and Direct Fluorescent-Antibody (DFA) requires a high level of technical skill, expensive equipment, and time-consuming. A method based on immunocytochemical (IC) using monoclonal antibody DSSE10 has several advantages. This study aimed to evaluate sensitivity and specificity IC assay compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR) as gold standard to detect Dengue Virus (DENV)-3 infections in mosquito Aedes aegypti.</p><p><strong>Methods</strong>: An experimental study was conducted in laboratory of Medical Parasitology, Faculty of Medicine, Universitas Gadjah Mada (UGM) in May 2009 until October 2010. A total of 22 artificially-infected adult Ae. aegypti mosquitoes of DENV 3 were used as infectious samples and 35 non-infected adult Ae. aegypti mosquitoes were used as normal ones. The IC Streptavidin Biotin Peroxidase Complex (SBPC) assay using monoclonal antibody DSSE10 was applied in mosquito head squash to detect Dengue virus antigen. RT-PCR as a gold standard was applied in mosquito thorax.</p><p><strong>Results</strong>: The kappa value showed a good agreement between two observers (kappa value 0.63). IC could detect dengue virus antigen as sensitive as RT-PCR (sensitivity 100%). But IC was less specific than RT-PCR (specificity 91%) because some false positive results were found in this method.</p><p><strong>Conclusion</strong>: The IC method has a high sensitivity and high specificity compared with RT-PCR. This IC method may be useful for virological surveillance of dengue infected Aedes mosquitoes. (Health Science Indones 2011;2:87-91).</p><p> </p>http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/88immunocytochemical, DSSE10, head squash, dengue |
| spellingShingle | Dyah Widiastuti Bambang Yunianto Sitti Rahmah Umniyati Nastiti Wijayanti Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito Health Science Journal of Indonesia immunocytochemical, DSSE10, head squash, dengue |
| title | Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito |
| title_full | Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito |
| title_fullStr | Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito |
| title_full_unstemmed | Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito |
| title_short | Sensitivity and specificity of immunocytochemical assay for detection of Dengue virus 3 infection in mosquito |
| title_sort | sensitivity and specificity of immunocytochemical assay for detection of dengue virus 3 infection in mosquito |
| topic | immunocytochemical, DSSE10, head squash, dengue |
| url | http://ejournal.litbang.depkes.go.id/index.php/HSJI/article/view/88 |
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