MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i>
Clubroot caused by <i>Plasmodiophora brassicae</i> is a severe disease of cruciferous crops that decreases crop quality and productivity. Several clubroot resistance-related quantitative trait loci and candidate genes have been identified. However, the underlying regulatory mechanism, th...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| author | Parameswari Paul Sushil Satish Chhapekar Jana Jeevan Rameneni Sang Heon Oh Vignesh Dhandapani Saminathan Subburaj Sang-Yoon Shin Nirala Ramchiary Chanseok Shin Su Ryun Choi Yong Pyo Lim |
| author_facet | Parameswari Paul Sushil Satish Chhapekar Jana Jeevan Rameneni Sang Heon Oh Vignesh Dhandapani Saminathan Subburaj Sang-Yoon Shin Nirala Ramchiary Chanseok Shin Su Ryun Choi Yong Pyo Lim |
| author_sort | Parameswari Paul |
| collection | DOAJ |
| description | Clubroot caused by <i>Plasmodiophora brassicae</i> is a severe disease of cruciferous crops that decreases crop quality and productivity. Several clubroot resistance-related quantitative trait loci and candidate genes have been identified. However, the underlying regulatory mechanism, the interrelationships among genes, and how genes are regulated remain unexplored. MicroRNAs (miRNAs) are attracting attention as regulators of gene expression, including during biotic stress responses. The main objective of this study was to understand how miRNAs regulate clubroot resistance-related genes in <i>P. brassicae</i>-infected <i>Brassica rapa</i>. Two <i>Brassica</i> miRNAs, Bra-miR1885a and Bra-miR1885b, were revealed to target TIR-NBS genes. In non-infected plants, both miRNAs were expressed at low levels to maintain the balance between plant development and basal immunity. However, their expression levels increased in <i>P. brassicae</i>-infected plants. Both miRNAs down-regulated the expression of the TIR-NBS genes <i>Bra019412</i> and <i>Bra019410</i>, which are located at a clubroot resistance-related quantitative trait locus. The Bra-miR1885-mediated down-regulation of both genes was detected for up to 15 days post-inoculation in the clubroot-resistant line CR Shinki and in the clubroot-susceptible line 94SK. A qRT-PCR analysis revealed <i>Bra019412</i> expression was negatively regulated by miR1885. Both <i>Bra019412</i> and <i>Bra019410</i> were more highly expressed in CR Shinki than in 94SK; the same expression pattern was detected in multiple clubroot-resistant and clubroot-susceptible inbred lines. A 5′ rapid amplification of cDNA ends analysis confirmed the cleavage of <i>Bra019412</i> by Bra-miR1885b. Thus, miR1885s potentially regulate TIR-NBS gene expression during <i>P. brassicae</i> infections of <i>B. rapa</i>. |
| first_indexed | 2024-03-10T08:10:59Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:10:59Z |
| publishDate | 2021-08-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9c582a5918fe4718821ebfc62b778bbc2023-11-22T10:43:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012217943310.3390/ijms22179433MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i>Parameswari Paul0Sushil Satish Chhapekar1Jana Jeevan Rameneni2Sang Heon Oh3Vignesh Dhandapani4Saminathan Subburaj5Sang-Yoon Shin6Nirala Ramchiary7Chanseok Shin8Su Ryun Choi9Yong Pyo Lim10Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaSchool of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, IndiaDepartment of Agricultural Biotechnology, Seoul National University, Seoul 08826, KoreaDepartment of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaDepartment of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaClubroot caused by <i>Plasmodiophora brassicae</i> is a severe disease of cruciferous crops that decreases crop quality and productivity. Several clubroot resistance-related quantitative trait loci and candidate genes have been identified. However, the underlying regulatory mechanism, the interrelationships among genes, and how genes are regulated remain unexplored. MicroRNAs (miRNAs) are attracting attention as regulators of gene expression, including during biotic stress responses. The main objective of this study was to understand how miRNAs regulate clubroot resistance-related genes in <i>P. brassicae</i>-infected <i>Brassica rapa</i>. Two <i>Brassica</i> miRNAs, Bra-miR1885a and Bra-miR1885b, were revealed to target TIR-NBS genes. In non-infected plants, both miRNAs were expressed at low levels to maintain the balance between plant development and basal immunity. However, their expression levels increased in <i>P. brassicae</i>-infected plants. Both miRNAs down-regulated the expression of the TIR-NBS genes <i>Bra019412</i> and <i>Bra019410</i>, which are located at a clubroot resistance-related quantitative trait locus. The Bra-miR1885-mediated down-regulation of both genes was detected for up to 15 days post-inoculation in the clubroot-resistant line CR Shinki and in the clubroot-susceptible line 94SK. A qRT-PCR analysis revealed <i>Bra019412</i> expression was negatively regulated by miR1885. Both <i>Bra019412</i> and <i>Bra019410</i> were more highly expressed in CR Shinki than in 94SK; the same expression pattern was detected in multiple clubroot-resistant and clubroot-susceptible inbred lines. A 5′ rapid amplification of cDNA ends analysis confirmed the cleavage of <i>Bra019412</i> by Bra-miR1885b. Thus, miR1885s potentially regulate TIR-NBS gene expression during <i>P. brassicae</i> infections of <i>B. rapa</i>.https://www.mdpi.com/1422-0067/22/17/9433MicroRNATIR-NBS genesQTLR gene<i>Brassica</i><i>Plasmodiophora brassicae</i> |
| spellingShingle | Parameswari Paul Sushil Satish Chhapekar Jana Jeevan Rameneni Sang Heon Oh Vignesh Dhandapani Saminathan Subburaj Sang-Yoon Shin Nirala Ramchiary Chanseok Shin Su Ryun Choi Yong Pyo Lim MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i> International Journal of Molecular Sciences MicroRNA TIR-NBS genes QTL R gene <i>Brassica</i> <i>Plasmodiophora brassicae</i> |
| title | MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i> |
| title_full | MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i> |
| title_fullStr | MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i> |
| title_full_unstemmed | MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i> |
| title_short | MiR1885 Regulates Disease Tolerance Genes in <i>Brassica rapa</i> during Early Infection with <i>Plasmodiophora brassicae</i> |
| title_sort | mir1885 regulates disease tolerance genes in i brassica rapa i during early infection with i plasmodiophora brassicae i |
| topic | MicroRNA TIR-NBS genes QTL R gene <i>Brassica</i> <i>Plasmodiophora brassicae</i> |
| url | https://www.mdpi.com/1422-0067/22/17/9433 |
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