AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia.
Several lines of evidence indicate altered trafficking of α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors in schizophrenia. Previous reports have shown potential changes in the trafficking of AMPA receptors based on subunit expression of endosomes, subcellular organelles located...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3375247?pdf=render |
| _version_ | 1828189444928176128 |
|---|---|
| author | John C Hammond James H Meador-Woodruff Vahram Haroutunian Robert E McCullumsmith |
| author_facet | John C Hammond James H Meador-Woodruff Vahram Haroutunian Robert E McCullumsmith |
| author_sort | John C Hammond |
| collection | DOAJ |
| description | Several lines of evidence indicate altered trafficking of α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors in schizophrenia. Previous reports have shown potential changes in the trafficking of AMPA receptors based on subunit expression of endosomes, subcellular organelles located near post-synaptic sites. We hypothesized that alterations in AMPA receptor trafficking through the endoplasmic reticulum (ER) may also be altered in schizophrenia. Accordingly, we developed a technique to isolate and measure content of the ER from postmortem brain tissue. We used Western blot and electron microscopy to show that we isolated an ER enriched fraction. We found no changes in the expression of the AMPA receptor subunits, GluR1-4, in the ER from the dorsolateral prefrontal cortex in schizophrenia. These data suggest that AMPA receptor trafficking through the ER is largely intact in schizophrenia. |
| first_indexed | 2024-04-12T08:05:54Z |
| format | Article |
| id | doaj.art-9c582fd1d091402a9a8e403af1e442fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T08:05:54Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9c582fd1d091402a9a8e403af1e442fc2022-12-22T03:41:10ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3919010.1371/journal.pone.0039190AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia.John C HammondJames H Meador-WoodruffVahram HaroutunianRobert E McCullumsmithSeveral lines of evidence indicate altered trafficking of α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate (AMPA) receptors in schizophrenia. Previous reports have shown potential changes in the trafficking of AMPA receptors based on subunit expression of endosomes, subcellular organelles located near post-synaptic sites. We hypothesized that alterations in AMPA receptor trafficking through the endoplasmic reticulum (ER) may also be altered in schizophrenia. Accordingly, we developed a technique to isolate and measure content of the ER from postmortem brain tissue. We used Western blot and electron microscopy to show that we isolated an ER enriched fraction. We found no changes in the expression of the AMPA receptor subunits, GluR1-4, in the ER from the dorsolateral prefrontal cortex in schizophrenia. These data suggest that AMPA receptor trafficking through the ER is largely intact in schizophrenia.http://europepmc.org/articles/PMC3375247?pdf=render |
| spellingShingle | John C Hammond James H Meador-Woodruff Vahram Haroutunian Robert E McCullumsmith AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia. PLoS ONE |
| title | AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia. |
| title_full | AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia. |
| title_fullStr | AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia. |
| title_full_unstemmed | AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia. |
| title_short | AMPA receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia. |
| title_sort | ampa receptor subunit expression in the endoplasmic reticulum in frontal cortex of elderly patients with schizophrenia |
| url | http://europepmc.org/articles/PMC3375247?pdf=render |
| work_keys_str_mv | AT johnchammond ampareceptorsubunitexpressionintheendoplasmicreticuluminfrontalcortexofelderlypatientswithschizophrenia AT jameshmeadorwoodruff ampareceptorsubunitexpressionintheendoplasmicreticuluminfrontalcortexofelderlypatientswithschizophrenia AT vahramharoutunian ampareceptorsubunitexpressionintheendoplasmicreticuluminfrontalcortexofelderlypatientswithschizophrenia AT robertemccullumsmith ampareceptorsubunitexpressionintheendoplasmicreticuluminfrontalcortexofelderlypatientswithschizophrenia |