Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector
Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine strains and recombinants were generated in primary (lamb) tes...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/10/1131 |
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| author | Michiel van Diepen Rosamund Chapman Nicola Douglass Leah Whittle Nicole Chineka Shireen Galant Christian Cotchobos Akiko Suzuki Anna-Lise Williamson |
| author_facet | Michiel van Diepen Rosamund Chapman Nicola Douglass Leah Whittle Nicole Chineka Shireen Galant Christian Cotchobos Akiko Suzuki Anna-Lise Williamson |
| author_sort | Michiel van Diepen |
| collection | DOAJ |
| description | Attenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine strains and recombinants were generated in primary (lamb) testis (LT) cells, Madin–Darby bovine kidney (MDBK) cells or in eggs. Growth in eggs is a laborious process, the use of primary cells has the potential to introduce pathogens and MDBK cells are known to harbor bovine viral diarrhea virus (BVDV). In this study, data is presented to show the growth of an attenuated LSDV strain in baby hamster kidney (BHK-21) cells. Subsequently, a recombinant LSDV vaccine was generated in BHK-21 cells. Partial growth was also observed in rabbit kidney cells (RK13), but only when the vaccinia virus host range gene K1L was expressed. Despite the limited growth, the expression of K1L was enough to serve as a positive selection marker for the generation of recombinant LSDV vaccines in RK13 cells. The simplification of generating (recombinant) LSDV vaccines shown here should increase the interest for this platform for future livestock vaccine development and, with BHK-21 cells approved for current good manufacturing practice, this can be expanded to human vaccines as well. |
| first_indexed | 2024-03-10T06:09:38Z |
| format | Article |
| id | doaj.art-9c59ef8bbcf449bebdf29890e981de3e |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T06:09:38Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-9c59ef8bbcf449bebdf29890e981de3e2023-11-22T20:15:38ZengMDPI AGVaccines2076-393X2021-10-01910113110.3390/vaccines9101131Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine VectorMichiel van Diepen0Rosamund Chapman1Nicola Douglass2Leah Whittle3Nicole Chineka4Shireen Galant5Christian Cotchobos6Akiko Suzuki7Anna-Lise Williamson8Department of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaDepartment of Pathology, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South AfricaAttenuated vaccine strains of lumpy skin disease virus (LSDV) have become increasingly popular as recombinant vaccine vectors, to target both LSDV, as well as other pathogens, including human infectious agents. Historically, these vaccine strains and recombinants were generated in primary (lamb) testis (LT) cells, Madin–Darby bovine kidney (MDBK) cells or in eggs. Growth in eggs is a laborious process, the use of primary cells has the potential to introduce pathogens and MDBK cells are known to harbor bovine viral diarrhea virus (BVDV). In this study, data is presented to show the growth of an attenuated LSDV strain in baby hamster kidney (BHK-21) cells. Subsequently, a recombinant LSDV vaccine was generated in BHK-21 cells. Partial growth was also observed in rabbit kidney cells (RK13), but only when the vaccinia virus host range gene K1L was expressed. Despite the limited growth, the expression of K1L was enough to serve as a positive selection marker for the generation of recombinant LSDV vaccines in RK13 cells. The simplification of generating (recombinant) LSDV vaccines shown here should increase the interest for this platform for future livestock vaccine development and, with BHK-21 cells approved for current good manufacturing practice, this can be expanded to human vaccines as well.https://www.mdpi.com/2076-393X/9/10/1131lumpy skin disease virusvaccine vectorBHK-21 cellsRK13 cellsK1L host range |
| spellingShingle | Michiel van Diepen Rosamund Chapman Nicola Douglass Leah Whittle Nicole Chineka Shireen Galant Christian Cotchobos Akiko Suzuki Anna-Lise Williamson Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector Vaccines lumpy skin disease virus vaccine vector BHK-21 cells RK13 cells K1L host range |
| title | Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector |
| title_full | Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector |
| title_fullStr | Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector |
| title_full_unstemmed | Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector |
| title_short | Advancements in the Growth and Construction of Recombinant Lumpy Skin Disease Virus (LSDV) for Use as a Vaccine Vector |
| title_sort | advancements in the growth and construction of recombinant lumpy skin disease virus lsdv for use as a vaccine vector |
| topic | lumpy skin disease virus vaccine vector BHK-21 cells RK13 cells K1L host range |
| url | https://www.mdpi.com/2076-393X/9/10/1131 |
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