Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis

Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis

Microfluidic cultivation devices that facilitate O2 control enable unique studies of the complex interplay between environmental O2 availability and microbial physiology at the single-cell level. Therefore, microbial single-cell analysis based on time-lapse microscopy is typically used to resolve mi...

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Bibliographic Details
Main Authors: Keitaro Kasahara, Markus Leygeber, Johannes Seiffarth, Karina Ruzaeva, Thomas Drepper, Katharina Nöh, Dietrich Kohlheyer
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Microbiology
Subjects:
microfluidic
oxygen control
FLIM
microbial single-cell analysis
PDMS
RTDP
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1198170/full

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