Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer
Abstract Recent evidence suggests that Fusobacterium nucleatum (Fn) is associated with the development and progression of colorectal cancer. We aimed to delineate the clinical implications of Fn in metastatic colon cancer. We performed quantitative polymerase chain reaction (qPCR) using DNA samples...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-98941-6 |
| _version_ | 1818743972170825728 |
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| author | Jii Bum Lee Kyung-A Kim Ho Yeon Cho DooA Kim Won Kyu Kim Dongeun Yong Hyukmin Lee Sang Sun Yoon Dai Hoon Han Yoon Dae Han Soonmyung Paik Mi Jang Han Sang Kim Joong Bae Ahn |
| author_facet | Jii Bum Lee Kyung-A Kim Ho Yeon Cho DooA Kim Won Kyu Kim Dongeun Yong Hyukmin Lee Sang Sun Yoon Dai Hoon Han Yoon Dae Han Soonmyung Paik Mi Jang Han Sang Kim Joong Bae Ahn |
| author_sort | Jii Bum Lee |
| collection | DOAJ |
| description | Abstract Recent evidence suggests that Fusobacterium nucleatum (Fn) is associated with the development and progression of colorectal cancer. We aimed to delineate the clinical implications of Fn in metastatic colon cancer. We performed quantitative polymerase chain reaction (qPCR) using DNA samples from synchronous metastatic colon cancer patients with either formalin-fixed paraffin-embedded (FFPE) archival primary site tumor samples or fresh colon tissues. Progression-free survival (PFS)1 and PFS2 were defined as PFS of first- and second-line palliative settings. qPCR for Fn was successfully performed using 112 samples (FFPE, n = 61; fresh tissue, n = 51). Forty-one and 68 patients had right-sided and left-sided colon cancer, respectively. Patients with Fn enriched right-sided colon cancers had shorter PFS1 (9.7 vs. 11.2 months) than the other subgroups (HR 3.54, 95% confidence interval [CI] 1.05–11.99; P = 0.04). Fn positive right-sided colon was also associated with shorter PFS2 (3.7 vs. 6.7 months; HR 2.34, 95% CI 0.69–7.91; P = 0.04). In the univariate analysis, PFS1 was affected by differentiation and Fn positive right-sided colon cancer. The multivariate analysis showed that differentiation (HR 2.68, 95% CI 1.40–5.14, P = 0.01) and Fn positive right-sided colon (HR 0.40, 95% CI 0.18–0.88, P = 0.02) were associated with PFS1. Fn enrichment in right sided colon was not associated with overall survival (OS). Fn enrichment has significantly worse prognosis in terms of PFS1 and PFS2 in patients with right-sided metastatic colon cancers. |
| first_indexed | 2024-12-18T02:36:54Z |
| format | Article |
| id | doaj.art-9c5dfceb244f4339ad02a8e78e20b689 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T02:36:54Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9c5dfceb244f4339ad02a8e78e20b6892022-12-21T21:23:46ZengNature PortfolioScientific Reports2045-23222021-10-011111910.1038/s41598-021-98941-6Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancerJii Bum Lee0Kyung-A Kim1Ho Yeon Cho2DooA Kim3Won Kyu Kim4Dongeun Yong5Hyukmin Lee6Sang Sun Yoon7Dai Hoon Han8Yoon Dae Han9Soonmyung Paik10Mi Jang11Han Sang Kim12Joong Bae Ahn13Division of Medical Oncology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of MedicineDivision of Medical Oncology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of MedicineGraduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of MedicineGraduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of MedicineNatural Products Research Center, Korea Institute of Science and TechnologyDepartment of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of MedicineDepartment of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of MedicineGraduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of MedicineDepartment of Surgery, Yonsei University College of MedicineDepartment of Surgery, Yonsei University College of MedicineGraduate School of Medical Science, Brain Korea 21 Project, Severance Biomedical Science Institute, Yonsei University College of MedicineDepartment of Pathology, Yonsei University College of MedicineDivision of Medical Oncology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of MedicineDivision of Medical Oncology, Department of Internal Medicine, Yonsei Cancer Center, Yonsei University College of MedicineAbstract Recent evidence suggests that Fusobacterium nucleatum (Fn) is associated with the development and progression of colorectal cancer. We aimed to delineate the clinical implications of Fn in metastatic colon cancer. We performed quantitative polymerase chain reaction (qPCR) using DNA samples from synchronous metastatic colon cancer patients with either formalin-fixed paraffin-embedded (FFPE) archival primary site tumor samples or fresh colon tissues. Progression-free survival (PFS)1 and PFS2 were defined as PFS of first- and second-line palliative settings. qPCR for Fn was successfully performed using 112 samples (FFPE, n = 61; fresh tissue, n = 51). Forty-one and 68 patients had right-sided and left-sided colon cancer, respectively. Patients with Fn enriched right-sided colon cancers had shorter PFS1 (9.7 vs. 11.2 months) than the other subgroups (HR 3.54, 95% confidence interval [CI] 1.05–11.99; P = 0.04). Fn positive right-sided colon was also associated with shorter PFS2 (3.7 vs. 6.7 months; HR 2.34, 95% CI 0.69–7.91; P = 0.04). In the univariate analysis, PFS1 was affected by differentiation and Fn positive right-sided colon cancer. The multivariate analysis showed that differentiation (HR 2.68, 95% CI 1.40–5.14, P = 0.01) and Fn positive right-sided colon (HR 0.40, 95% CI 0.18–0.88, P = 0.02) were associated with PFS1. Fn enrichment in right sided colon was not associated with overall survival (OS). Fn enrichment has significantly worse prognosis in terms of PFS1 and PFS2 in patients with right-sided metastatic colon cancers.https://doi.org/10.1038/s41598-021-98941-6 |
| spellingShingle | Jii Bum Lee Kyung-A Kim Ho Yeon Cho DooA Kim Won Kyu Kim Dongeun Yong Hyukmin Lee Sang Sun Yoon Dai Hoon Han Yoon Dae Han Soonmyung Paik Mi Jang Han Sang Kim Joong Bae Ahn Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer Scientific Reports |
| title | Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer |
| title_full | Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer |
| title_fullStr | Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer |
| title_full_unstemmed | Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer |
| title_short | Association between Fusobacterium nucleatum and patient prognosis in metastatic colon cancer |
| title_sort | association between fusobacterium nucleatum and patient prognosis in metastatic colon cancer |
| url | https://doi.org/10.1038/s41598-021-98941-6 |
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