Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy
ABSTRACT: Objectives: Ceftolozane/tazobactam (C/T) is a novel cephalosporin and β-lactamase inhibitor combination with great activity against Pseudomonas aeruginosa. To assess P. aeruginosa susceptibility to C/T, a surveillance study was conducted from October 2018 to March 2019 at the University H...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | Journal of Global Antimicrobial Resistance |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221371652200176X |
| _version_ | 1818479504707813376 |
|---|---|
| author | Gianluca Morroni Lucia Brescini Alberto Antonelli Vincenzo Di Pilato Sefora Castelletti Andrea Brenciani Gloria D'Achille Marina Mingoia Eleonora Giovanetti Simona Fioriti Annamaria Masucci Tommaso Giani Andrea Giacometti Gian Maria Rossolini Oscar Cirioni |
| author_facet | Gianluca Morroni Lucia Brescini Alberto Antonelli Vincenzo Di Pilato Sefora Castelletti Andrea Brenciani Gloria D'Achille Marina Mingoia Eleonora Giovanetti Simona Fioriti Annamaria Masucci Tommaso Giani Andrea Giacometti Gian Maria Rossolini Oscar Cirioni |
| author_sort | Gianluca Morroni |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Ceftolozane/tazobactam (C/T) is a novel cephalosporin and β-lactamase inhibitor combination with great activity against Pseudomonas aeruginosa. To assess P. aeruginosa susceptibility to C/T, a surveillance study was conducted from October 2018 to March 2019 at the University Hospital ‘Ospedali Riuniti’ in Ancona, Italy. Methods: Minimum inhibitory concentrations (MICs) to C/T were determined by Etest strip. Resistant isolates were characterized by phenotypic (broth microdilution antimicrobial susceptibility testing and modified Carbapenem Inactivation Method [mCIM]) and genotypic (Polymerase Chain Reaction [PCR], Pulsed Field Gel Electrophoresis [PFGE], and whole-genome sequencing [WGS]) methods. Clinical variables of patients infected by C/T-resistant P. aeruginosa were collected from medical records. Results: Fifteen of 317 P. aeruginosa collected showed resistance to C/T (4.7%). Ten strains demonstrated carbapenemase activity by mCIM method, and PCR confirmed that eight strains harbored a blaVIM gene while the other two were positive for blaIMP. Additionally, three isolates carried acquired extended spectrum β-lactamase genes (two isolates carried blaPER and one carried blaGES). Eight strains were strictly related by PFGE and WGS analysis confirmed that they belonged to sequence type (ST)111. The other STs found were ST175 (two isolates), ST235 (two isolates), ST70 (one isolate), ST621 (one isolate), and the new ST3354 (one isolate). Most patients had received previous antibiotic therapies, carried invasive devices, and experienced prolonged hospitalization. Conclusion: This study demonstrated the presence of C/T-resistant P. aeruginosa isolates in a regional hospital carrying a number of resistance mechanisms acquired by different high-risk clones. |
| first_indexed | 2024-12-10T11:11:32Z |
| format | Article |
| id | doaj.art-9c5e38bad24e4b8caaf4d1b174461bd2 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-10T11:11:32Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-9c5e38bad24e4b8caaf4d1b174461bd22022-12-22T01:51:24ZengElsevierJournal of Global Antimicrobial Resistance2213-71652022-09-0130377383Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central ItalyGianluca Morroni0Lucia Brescini1Alberto Antonelli2Vincenzo Di Pilato3Sefora Castelletti4Andrea Brenciani5Gloria D'Achille6Marina Mingoia7Eleonora Giovanetti8Simona Fioriti9Annamaria Masucci10Tommaso Giani11Andrea Giacometti12Gian Maria Rossolini13Oscar Cirioni14Microbiology Unit, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyInfectious Diseases Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, Italy; Corresponding author. Mailing address: Infectious Diseases Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, via Conca 71, 60126, Ancona, Italy.Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, ItalyDepartment of Surgical Sciences and Integrated Diagnostics, University of Genoa, Genoa, ItalyInfectious Diseases Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyMicrobiology Unit, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyMicrobiology Unit, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyMicrobiology Unit, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyMicrobiology Unit, Department of Life and Environmental Sciences, Polytechnic University of Marche, Ancona, ItalyInfectious Diseases Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyClinical Microbiology Laboratory, University Hospital ‘Ospedali Riuniti’, Ancona, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, ItalyInfectious Diseases Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, Florence, Italy; Microbiology and Virology Unit, Florence Careggi University Hospital, Florence, ItalyInfectious Diseases Clinic, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche, Ancona, ItalyABSTRACT: Objectives: Ceftolozane/tazobactam (C/T) is a novel cephalosporin and β-lactamase inhibitor combination with great activity against Pseudomonas aeruginosa. To assess P. aeruginosa susceptibility to C/T, a surveillance study was conducted from October 2018 to March 2019 at the University Hospital ‘Ospedali Riuniti’ in Ancona, Italy. Methods: Minimum inhibitory concentrations (MICs) to C/T were determined by Etest strip. Resistant isolates were characterized by phenotypic (broth microdilution antimicrobial susceptibility testing and modified Carbapenem Inactivation Method [mCIM]) and genotypic (Polymerase Chain Reaction [PCR], Pulsed Field Gel Electrophoresis [PFGE], and whole-genome sequencing [WGS]) methods. Clinical variables of patients infected by C/T-resistant P. aeruginosa were collected from medical records. Results: Fifteen of 317 P. aeruginosa collected showed resistance to C/T (4.7%). Ten strains demonstrated carbapenemase activity by mCIM method, and PCR confirmed that eight strains harbored a blaVIM gene while the other two were positive for blaIMP. Additionally, three isolates carried acquired extended spectrum β-lactamase genes (two isolates carried blaPER and one carried blaGES). Eight strains were strictly related by PFGE and WGS analysis confirmed that they belonged to sequence type (ST)111. The other STs found were ST175 (two isolates), ST235 (two isolates), ST70 (one isolate), ST621 (one isolate), and the new ST3354 (one isolate). Most patients had received previous antibiotic therapies, carried invasive devices, and experienced prolonged hospitalization. Conclusion: This study demonstrated the presence of C/T-resistant P. aeruginosa isolates in a regional hospital carrying a number of resistance mechanisms acquired by different high-risk clones.http://www.sciencedirect.com/science/article/pii/S221371652200176XCeftolozane/tazobactamPseudomonas aeruginosaβ-lactamase |
| spellingShingle | Gianluca Morroni Lucia Brescini Alberto Antonelli Vincenzo Di Pilato Sefora Castelletti Andrea Brenciani Gloria D'Achille Marina Mingoia Eleonora Giovanetti Simona Fioriti Annamaria Masucci Tommaso Giani Andrea Giacometti Gian Maria Rossolini Oscar Cirioni Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy Journal of Global Antimicrobial Resistance Ceftolozane/tazobactam Pseudomonas aeruginosa β-lactamase |
| title | Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy |
| title_full | Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy |
| title_fullStr | Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy |
| title_full_unstemmed | Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy |
| title_short | Clinical and microbiological features of ceftolozane/tazobactam-resistant Pseudomonas aeruginosa isolates in a university hospital in central Italy |
| title_sort | clinical and microbiological features of ceftolozane tazobactam resistant pseudomonas aeruginosa isolates in a university hospital in central italy |
| topic | Ceftolozane/tazobactam Pseudomonas aeruginosa β-lactamase |
| url | http://www.sciencedirect.com/science/article/pii/S221371652200176X |
| work_keys_str_mv | AT gianlucamorroni clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT luciabrescini clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT albertoantonelli clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT vincenzodipilato clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT seforacastelletti clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT andreabrenciani clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT gloriadachille clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT marinamingoia clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT eleonoragiovanetti clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT simonafioriti clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT annamariamasucci clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT tommasogiani clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT andreagiacometti clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT gianmariarossolini clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly AT oscarcirioni clinicalandmicrobiologicalfeaturesofceftolozanetazobactamresistantpseudomonasaeruginosaisolatesinauniversityhospitalincentralitaly |