Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma
Background: Primary open angle glaucoma (POAG) is a progressive optic nerve degeneration, leading to irreversible visual damage. Alterations of the aqueous humor (AH), the biological fluid filling both the anterior and the posterior chambers of the eye, play a pathogenic role in POAG. AH protein com...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022010416 |
| _version_ | 1811334937918308352 |
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| author | A. Pulliero A. Izzotti L. Pastorino S. Gandolfi |
| author_facet | A. Pulliero A. Izzotti L. Pastorino S. Gandolfi |
| author_sort | A. Pulliero |
| collection | DOAJ |
| description | Background: Primary open angle glaucoma (POAG) is a progressive optic nerve degeneration, leading to irreversible visual damage. Alterations of the aqueous humor (AH), the biological fluid filling both the anterior and the posterior chambers of the eye, play a pathogenic role in POAG. AH protein composition is altered during glaucoma progression. Nestin protein was found to be differentially expressed in the AH of glaucomatous patients compared to unaffected matched controls. Methods: Nestin was analyzed by an open quartz crystal microbalance (QCM) in the AH of 21 glaucomatous patients compared to nine unaffected controls. The surface of the electrode used in the QCM was coated with an analyte-specific recognition layer. Results: Positive nestin values were recorded in the AH collected from POAG patients; negative values of nestin detection were obtained by analyzing the AH collected from non-POAG glaucomatous patients and unaffected controls. Conclusion: The present study proposes and validates a new clinically applicable approach to analyze biological markers in AH for POAG diagnosis. |
| first_indexed | 2024-04-13T17:16:42Z |
| format | Article |
| id | doaj.art-9c5fe3d872f042d1b5b84846d400b56f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-13T17:16:42Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-9c5fe3d872f042d1b5b84846d400b56f2022-12-22T02:38:06ZengElsevierHeliyon2405-84402022-06-0186e09753Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucomaA. Pulliero0A. Izzotti1L. Pastorino2S. Gandolfi3Department of Health Sciences, University of Genoa, Via A. Pastore 1 Genoa, Italy; Corresponding author.UOC Mutagenesis and Cancer Prevention, IRCCS Ospedale Policlinico San Martino, Largo Rosanna Benzi, 10, Genoa, Italy; Department of Experimental Medicine, University of Genoa, ItalyDepartment of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genova, Via all’ Opera Pia 13, 16145 Genoa, ItalyOphthalmology Unit, Department of Biological Biotechnological and Translational Sciences, University of Parma, Parma, ItalyBackground: Primary open angle glaucoma (POAG) is a progressive optic nerve degeneration, leading to irreversible visual damage. Alterations of the aqueous humor (AH), the biological fluid filling both the anterior and the posterior chambers of the eye, play a pathogenic role in POAG. AH protein composition is altered during glaucoma progression. Nestin protein was found to be differentially expressed in the AH of glaucomatous patients compared to unaffected matched controls. Methods: Nestin was analyzed by an open quartz crystal microbalance (QCM) in the AH of 21 glaucomatous patients compared to nine unaffected controls. The surface of the electrode used in the QCM was coated with an analyte-specific recognition layer. Results: Positive nestin values were recorded in the AH collected from POAG patients; negative values of nestin detection were obtained by analyzing the AH collected from non-POAG glaucomatous patients and unaffected controls. Conclusion: The present study proposes and validates a new clinically applicable approach to analyze biological markers in AH for POAG diagnosis.http://www.sciencedirect.com/science/article/pii/S2405844022010416Primary open angle glaucomaEarly diagnosisOpen quartz crystal microbalance (QCM)Chemical coating on gold surfaceQuartz sensor |
| spellingShingle | A. Pulliero A. Izzotti L. Pastorino S. Gandolfi Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma Heliyon Primary open angle glaucoma Early diagnosis Open quartz crystal microbalance (QCM) Chemical coating on gold surface Quartz sensor |
| title | Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma |
| title_full | Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma |
| title_fullStr | Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma |
| title_full_unstemmed | Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma |
| title_short | Analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma |
| title_sort | analysis of nestin protein in the aqueous humor as biomarker of open angle glaucoma |
| topic | Primary open angle glaucoma Early diagnosis Open quartz crystal microbalance (QCM) Chemical coating on gold surface Quartz sensor |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022010416 |
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