Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes
The tight interaction between somatic and germline cells is conserved in animal spermatogenesis. The testes of Drosophila melanogaster are the model of choice to identify processes responsible for mature gamete production. However, processes of differentiation and soma-germline interactions occurrin...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933522000498 |
| _version_ | 1811315792666427392 |
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| author | Vladimir E. Adashev Sergei S. Bazylev Daria M. Potashnikova Baira K. Godneeva Aleksei S. Shatskikh Oxana M. Olenkina Ludmila V. Olenina Alexei A. Kotov |
| author_facet | Vladimir E. Adashev Sergei S. Bazylev Daria M. Potashnikova Baira K. Godneeva Aleksei S. Shatskikh Oxana M. Olenkina Ludmila V. Olenina Alexei A. Kotov |
| author_sort | Vladimir E. Adashev |
| collection | DOAJ |
| description | The tight interaction between somatic and germline cells is conserved in animal spermatogenesis. The testes of Drosophila melanogaster are the model of choice to identify processes responsible for mature gamete production. However, processes of differentiation and soma-germline interactions occurring in somatic cyst cells are currently understudied. Here we focused on the comparison of transcriptome expression patterns of early and mature somatic cyst cells to find out the developmental changes taking place in them. We employed a FACS-based approach for the isolation of early and mature somatic cyst cells from fly testes, subsequent preparation of RNA-Seq libraries, and analysis of gene differential expression in the sorted cells. We found increased expression of genes involved in cell cycle-related processes in early cyst cells, which is necessary for the proliferation and self-renewal of a crucial population of early cyst cells, cyst stem cells. Genes proposedly required for lamellipodium-like projection organization for proper cyst formation were also detected among the upregulated ones in early cyst cells. Gene Ontology and interactome analyses of upregulated genes in mature cyst cells revealed a striking over-representation of gene categories responsible for metabolic and catabolic cellular processes, as well as genes supporting the energetic state of the cells provided by oxidative phosphorylation that is carried out in mitochondria. Our comparative analyses of differentially expressed genes revealed major peculiarities in early and mature cyst cells and provide novel insight into their regulation, which is important for male fertility. |
| first_indexed | 2024-04-13T11:37:45Z |
| format | Article |
| id | doaj.art-9c606ea434b241efac0d22319f088603 |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-04-13T11:37:45Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-9c606ea434b241efac0d22319f0886032022-12-22T02:48:25ZengElsevierEuropean Journal of Cell Biology0171-93352022-06-011013151246Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testesVladimir E. Adashev0Sergei S. Bazylev1Daria M. Potashnikova2Baira K. Godneeva3Aleksei S. Shatskikh4Oxana M. Olenkina5Ludmila V. Olenina6Alexei A. Kotov7Institute of Molecular Genetics of National Research Center ''Kurchatov Institute'', 2 Kurchatov Sq., Moscow 123182, RussiaInstitute of Molecular Genetics of National Research Center ''Kurchatov Institute'', 2 Kurchatov Sq., Moscow 123182, RussiaLomonosov Moscow State University, School of Biology, Department of Cell Biology and Histology, Moscow 119234, RussiaInstitute of Molecular Genetics of National Research Center ''Kurchatov Institute'', 2 Kurchatov Sq., Moscow 123182, RussiaInstitute of Molecular Genetics of National Research Center ''Kurchatov Institute'', 2 Kurchatov Sq., Moscow 123182, RussiaInstitute of Molecular Genetics of National Research Center ''Kurchatov Institute'', 2 Kurchatov Sq., Moscow 123182, RussiaInstitute of Molecular Genetics of National Research Center ''Kurchatov Institute'', 2 Kurchatov Sq., Moscow 123182, Russia; Corresponding authors.Institute of Molecular Genetics of National Research Center ''Kurchatov Institute'', 2 Kurchatov Sq., Moscow 123182, Russia; Corresponding authors.The tight interaction between somatic and germline cells is conserved in animal spermatogenesis. The testes of Drosophila melanogaster are the model of choice to identify processes responsible for mature gamete production. However, processes of differentiation and soma-germline interactions occurring in somatic cyst cells are currently understudied. Here we focused on the comparison of transcriptome expression patterns of early and mature somatic cyst cells to find out the developmental changes taking place in them. We employed a FACS-based approach for the isolation of early and mature somatic cyst cells from fly testes, subsequent preparation of RNA-Seq libraries, and analysis of gene differential expression in the sorted cells. We found increased expression of genes involved in cell cycle-related processes in early cyst cells, which is necessary for the proliferation and self-renewal of a crucial population of early cyst cells, cyst stem cells. Genes proposedly required for lamellipodium-like projection organization for proper cyst formation were also detected among the upregulated ones in early cyst cells. Gene Ontology and interactome analyses of upregulated genes in mature cyst cells revealed a striking over-representation of gene categories responsible for metabolic and catabolic cellular processes, as well as genes supporting the energetic state of the cells provided by oxidative phosphorylation that is carried out in mitochondria. Our comparative analyses of differentially expressed genes revealed major peculiarities in early and mature cyst cells and provide novel insight into their regulation, which is important for male fertility.http://www.sciencedirect.com/science/article/pii/S0171933522000498DrosophilaSpermatogenesisSomatic cyst cellsTranscriptome analysisInteraction networks |
| spellingShingle | Vladimir E. Adashev Sergei S. Bazylev Daria M. Potashnikova Baira K. Godneeva Aleksei S. Shatskikh Oxana M. Olenkina Ludmila V. Olenina Alexei A. Kotov Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes European Journal of Cell Biology Drosophila Spermatogenesis Somatic cyst cells Transcriptome analysis Interaction networks |
| title | Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes |
| title_full | Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes |
| title_fullStr | Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes |
| title_full_unstemmed | Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes |
| title_short | Comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of Drosophila testes |
| title_sort | comparative transcriptional analysis uncovers molecular processes in early and mature somatic cyst cells of drosophila testes |
| topic | Drosophila Spermatogenesis Somatic cyst cells Transcriptome analysis Interaction networks |
| url | http://www.sciencedirect.com/science/article/pii/S0171933522000498 |
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