Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors
Serological testing is a tool to predict protection against later infection. This potential heavily relies on antibody levels showing acceptable agreement with gold standard virus neutralization tests. The aim of our study was to investigate diagnostic value of the available serological tests in ter...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-05-01
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| Series: | Practical Laboratory Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551721000226 |
| _version_ | 1819135867405467648 |
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| author | Zsófia Szabó Tamás Szabó Kornélia Bodó Gábor Kemenesi Fanni Földes Katalin Kristóf Eszter Barabás Barna Vásárhelyi Zoltán Prohászka Eszter Fodor Ferenc Jakab Timea Berki Zsombor Lacza |
| author_facet | Zsófia Szabó Tamás Szabó Kornélia Bodó Gábor Kemenesi Fanni Földes Katalin Kristóf Eszter Barabás Barna Vásárhelyi Zoltán Prohászka Eszter Fodor Ferenc Jakab Timea Berki Zsombor Lacza |
| author_sort | Zsófia Szabó |
| collection | DOAJ |
| description | Serological testing is a tool to predict protection against later infection. This potential heavily relies on antibody levels showing acceptable agreement with gold standard virus neutralization tests. The aim of our study was to investigate diagnostic value of the available serological tests in terms of predicting virus neutralizing activity of serum samples drawn 5–7 weeks after onset of symptoms from 101 donors with a history of COVID-19.Immune responses against Receptor Binding Domain (RBD), Spike1 and 2 proteins and Nucleocapsid antigens were measured by various ELISA tests. Neutralizing antibody activity in serum samples was assessed by a cell-based virus neutralization test.Spearman correlation coefficients between serological and neutralization results ranged from 0.41 to 0.91 indicating moderate to strong correlation between ELISA test results and virus neutralization. The sensitivity and specificity of ELISA tests in the prediction of neutralization were 35–100% and 35–90% respectively. No clear cut off levels can be established that would reliably indicate neutralization activity. For some tests, however, a value below which the sample is not expected to neutralize can be established. Our data suggests that several of the ELISA kits tested may be suitable for epidemiological surveys 1–2 months after the infection, estimating whether a person may have recently exposed to the virus. Sensitivities considerably superseding specificity at the cut-off values proposed by the manufacturers suggest greater potential in the identification of insufficient antibody responses than in confirming protection. Nevertheless, the former might be important in assessing response to vaccination and characterizing therapeutic plasma preparations. |
| first_indexed | 2024-12-22T10:25:54Z |
| format | Article |
| id | doaj.art-9c66657dbb8c4826a99bc2bbe7a45372 |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-12-22T10:25:54Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-9c66657dbb8c4826a99bc2bbe7a453722022-12-21T18:29:29ZengElsevierPractical Laboratory Medicine2352-55172021-05-0125e00222Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donorsZsófia Szabó0Tamás Szabó1Kornélia Bodó2Gábor Kemenesi3Fanni Földes4Katalin Kristóf5Eszter Barabás6Barna Vásárhelyi7Zoltán Prohászka8Eszter Fodor9Ferenc Jakab10Timea Berki11Zsombor Lacza12Department of Laboratory Medicine, Semmelweis University, Budapest, Hungary; Corresponding author. Department of Laboratory Medicine, Semmelweis University, Budapest, 1082, Üllői str. 78, Hungary.Department of Laboratory Medicine, Semmelweis University, Budapest, Hungary; Institute of Developmental Immunology, Medical University of Innsbruck, Innsbruck, AustriaDepartment of Immunology and Biotechnology, Clinical Centre, University of Pécs, Pécs, HungaryNational Laboratory of Virology, BSL-4 Laboratory, Szentágothai Research Centre, University of Pécs, Pécs, Hungary; Institute of Biology, Faculty of Sciences, University of Pécs, 7622, Pécs, HungaryNational Laboratory of Virology, BSL-4 Laboratory, Szentágothai Research Centre, University of Pécs, Pécs, Hungary; Institute of Biology, Faculty of Sciences, University of Pécs, 7622, Pécs, HungaryDepartment of Laboratory Medicine, Semmelweis University, Budapest, HungaryDepartment of Laboratory Medicine, Semmelweis University, Budapest, HungaryDepartment of Laboratory Medicine, Semmelweis University, Budapest, HungaryDepartment of Internal Medicine and Hematology, Semmelweis University, Budapest and Research Group for Immunology and Hematology, Semmelweis University- Eötvös Loránd Research Network (Office for Supported Research Groups), Budapest, HungaryInstitute of Sports and Health Sciences, University of Physical Education, 1112, Budapest, HungaryNational Laboratory of Virology, BSL-4 Laboratory, Szentágothai Research Centre, University of Pécs, Pécs, Hungary; Institute of Biology, Faculty of Sciences, University of Pécs, 7622, Pécs, HungaryDepartment of Immunology and Biotechnology, Clinical Centre, University of Pécs, Pécs, HungaryInstitute of Sports and Health Sciences, University of Physical Education, 1112, Budapest, HungarySerological testing is a tool to predict protection against later infection. This potential heavily relies on antibody levels showing acceptable agreement with gold standard virus neutralization tests. The aim of our study was to investigate diagnostic value of the available serological tests in terms of predicting virus neutralizing activity of serum samples drawn 5–7 weeks after onset of symptoms from 101 donors with a history of COVID-19.Immune responses against Receptor Binding Domain (RBD), Spike1 and 2 proteins and Nucleocapsid antigens were measured by various ELISA tests. Neutralizing antibody activity in serum samples was assessed by a cell-based virus neutralization test.Spearman correlation coefficients between serological and neutralization results ranged from 0.41 to 0.91 indicating moderate to strong correlation between ELISA test results and virus neutralization. The sensitivity and specificity of ELISA tests in the prediction of neutralization were 35–100% and 35–90% respectively. No clear cut off levels can be established that would reliably indicate neutralization activity. For some tests, however, a value below which the sample is not expected to neutralize can be established. Our data suggests that several of the ELISA kits tested may be suitable for epidemiological surveys 1–2 months after the infection, estimating whether a person may have recently exposed to the virus. Sensitivities considerably superseding specificity at the cut-off values proposed by the manufacturers suggest greater potential in the identification of insufficient antibody responses than in confirming protection. Nevertheless, the former might be important in assessing response to vaccination and characterizing therapeutic plasma preparations.http://www.sciencedirect.com/science/article/pii/S2352551721000226SARS-CoV-2COVID-19Correlate of protectionSerological testAntibody responseNeutralization |
| spellingShingle | Zsófia Szabó Tamás Szabó Kornélia Bodó Gábor Kemenesi Fanni Földes Katalin Kristóf Eszter Barabás Barna Vásárhelyi Zoltán Prohászka Eszter Fodor Ferenc Jakab Timea Berki Zsombor Lacza Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors Practical Laboratory Medicine SARS-CoV-2 COVID-19 Correlate of protection Serological test Antibody response Neutralization |
| title | Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors |
| title_full | Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors |
| title_fullStr | Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors |
| title_full_unstemmed | Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors |
| title_short | Comparison of virus neutralization activity and results of 10 different anti-SARS-CoV-2 serological tests in COVID-19 recovered plasma donors |
| title_sort | comparison of virus neutralization activity and results of 10 different anti sars cov 2 serological tests in covid 19 recovered plasma donors |
| topic | SARS-CoV-2 COVID-19 Correlate of protection Serological test Antibody response Neutralization |
| url | http://www.sciencedirect.com/science/article/pii/S2352551721000226 |
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