CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestations
The existence of multiple autoimmune disorders in diabetics may indicate underlying primary defects of immune regulation. The study aims at estimation of defects of CD4+ CD25+high cells among diabetic children with multiple autoimmune manifestations, and identification of disease characteristics in...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-11-01
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| Series: | Journal of Advanced Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2090123213001185 |
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| author | Dalia S. Abd Elaziz Mona H. Hafez Nermeen M. Galal Safa S. Meshaal Aisha M. El Marsafy |
| author_facet | Dalia S. Abd Elaziz Mona H. Hafez Nermeen M. Galal Safa S. Meshaal Aisha M. El Marsafy |
| author_sort | Dalia S. Abd Elaziz |
| collection | DOAJ |
| description | The existence of multiple autoimmune disorders in diabetics may indicate underlying primary defects of immune regulation. The study aims at estimation of defects of CD4+ CD25+high cells among diabetic children with multiple autoimmune manifestations, and identification of disease characteristics in those children. Twenty-two cases with type 1 diabetes associated with other autoimmune diseases were recruited from the Diabetic Endocrine and Metabolic Pediatric Unit (DEMPU), Cairo University along with twenty-one normal subjects matched for age and sex as a control group. Their anthropometric measurements, diabetic profiles and glycemic control were recorded. Laboratory investigations included complete blood picture, glycosylated hemoglobin, antithyroid antibodies, celiac antibody panel and inflammatory bowel disease markers when indicated. Flow cytometric analysis of T-cell subpopulation was performed using anti-CD3, anti-CD4, anti-CD8, anti-CD25 monoclonal antibodies. Three cases revealed a proportion of CD4+ CD25+high below 0.1% and one case had zero counts. However, this observation did not mount to a significant statistical difference between the case and control groups neither in percentage nor absolute numbers. Significant statistical differences were observed between the case and the control groups regarding their height, weight centiles, as well as hemoglobin percentage, white cell counts and the absolute lymphocytic counts. We concluded that, derangements of CD4+ CD25+high cells may exist among diabetic children with multiple autoimmune manifestations indicating defects of immune controllers. |
| first_indexed | 2024-12-12T11:17:13Z |
| format | Article |
| id | doaj.art-9c6e300e9c87430c8b54702418d2b9bd |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-12-12T11:17:13Z |
| publishDate | 2014-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-9c6e300e9c87430c8b54702418d2b9bd2022-12-22T00:26:07ZengElsevierJournal of Advanced Research2090-12322014-11-015664765510.1016/j.jare.2013.09.004CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestationsDalia S. Abd Elaziz0Mona H. Hafez1Nermeen M. Galal2Safa S. Meshaal3Aisha M. El Marsafy4Pediatric Department, Faculty of Medicine, Cairo University, EgyptPediatric Department, Faculty of Medicine, Cairo University, EgyptPediatric Department, Faculty of Medicine, Cairo University, EgyptClinical and Chemical Pathology, Faculty of Medicine, Cairo University, EgyptPediatric Department, Faculty of Medicine, Cairo University, EgyptThe existence of multiple autoimmune disorders in diabetics may indicate underlying primary defects of immune regulation. The study aims at estimation of defects of CD4+ CD25+high cells among diabetic children with multiple autoimmune manifestations, and identification of disease characteristics in those children. Twenty-two cases with type 1 diabetes associated with other autoimmune diseases were recruited from the Diabetic Endocrine and Metabolic Pediatric Unit (DEMPU), Cairo University along with twenty-one normal subjects matched for age and sex as a control group. Their anthropometric measurements, diabetic profiles and glycemic control were recorded. Laboratory investigations included complete blood picture, glycosylated hemoglobin, antithyroid antibodies, celiac antibody panel and inflammatory bowel disease markers when indicated. Flow cytometric analysis of T-cell subpopulation was performed using anti-CD3, anti-CD4, anti-CD8, anti-CD25 monoclonal antibodies. Three cases revealed a proportion of CD4+ CD25+high below 0.1% and one case had zero counts. However, this observation did not mount to a significant statistical difference between the case and control groups neither in percentage nor absolute numbers. Significant statistical differences were observed between the case and the control groups regarding their height, weight centiles, as well as hemoglobin percentage, white cell counts and the absolute lymphocytic counts. We concluded that, derangements of CD4+ CD25+high cells may exist among diabetic children with multiple autoimmune manifestations indicating defects of immune controllers.http://www.sciencedirect.com/science/article/pii/S2090123213001185CD4+ CD25+ cellsAutoimmunityType1 diabetes mellitusT regulatory cellsChildren |
| spellingShingle | Dalia S. Abd Elaziz Mona H. Hafez Nermeen M. Galal Safa S. Meshaal Aisha M. El Marsafy CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestations Journal of Advanced Research CD4+ CD25+ cells Autoimmunity Type1 diabetes mellitus T regulatory cells Children |
| title | CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestations |
| title_full | CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestations |
| title_fullStr | CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestations |
| title_full_unstemmed | CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestations |
| title_short | CD4+ CD25+ cells in type 1 diabetic patients with other autoimmune manifestations |
| title_sort | cd4 cd25 cells in type 1 diabetic patients with other autoimmune manifestations |
| topic | CD4+ CD25+ cells Autoimmunity Type1 diabetes mellitus T regulatory cells Children |
| url | http://www.sciencedirect.com/science/article/pii/S2090123213001185 |
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