Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure
Objectives and Methods. It is shown that long-term cryopreservation for 2 and 4 years leads to a reduction in the number and viability of the mononuclear cells obtained after mobilization granulocyte colony-stimulating factor (G-CSF) from patients with chronic heart failure. In addition, during prol...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Meshalkin National Medical Research Center
2015-10-01
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| Series: | Патология кровообращения и кардиохирургия |
| Subjects: | |
| Online Access: | http://journalmeshalkin.ru/index.php/heartjournal/article/view/12 |
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| author | Н. А. Бондаренко А. П. Лыков О. В. Повещенко А. Ф. Повещенко Е. А. Покушалов А. М. Караськов В. И. Коненков И. И. Ким А. Б. Романов |
| author_facet | Н. А. Бондаренко А. П. Лыков О. В. Повещенко А. Ф. Повещенко Е. А. Покушалов А. М. Караськов В. И. Коненков И. И. Ким А. Б. Романов |
| author_sort | Н. А. Бондаренко |
| collection | DOAJ |
| description | Objectives and Methods. It is shown that long-term cryopreservation for 2 and 4 years leads to a reduction in the number and viability of the mononuclear cells obtained after mobilization granulocyte colony-stimulating factor (G-CSF) from patients with chronic heart failure. In addition, during prolonged cryopreservation decreased amount of content endothelial progenitor cells of the mononuclear cells from patients with chronic heart failure. Results. It was established that in cryopreservation for 2 and 4 years leads to a decreased proliferative activity of the mononuclear cells mobilized by G-CSF, both spontaneous and mitogen stimulated, increase cell number in the stage of apoptosis. The findings suggest that the negative impact on the long cryopreservation mononuclear cells obtained after G-CSF mobilization and effective cell therapy for heart failure patients need to use cells in the cryopreservation of not more than 2 years. |
| first_indexed | 2024-03-12T20:00:00Z |
| format | Article |
| id | doaj.art-9c6ea5ebbaf040adacbb6b4f76d973a0 |
| institution | Directory Open Access Journal |
| issn | 1681-3472 2500-3119 |
| language | English |
| last_indexed | 2024-03-12T20:00:00Z |
| publishDate | 2015-10-01 |
| publisher | Meshalkin National Medical Research Center |
| record_format | Article |
| series | Патология кровообращения и кардиохирургия |
| spelling | doaj.art-9c6ea5ebbaf040adacbb6b4f76d973a02023-08-02T02:30:08ZengMeshalkin National Medical Research CenterПатология кровообращения и кардиохирургия1681-34722500-31192015-10-01191909410.21688/1681-3472-2015-1-90-94732Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failureН. А. Бондаренко0А. П. ЛыковО. В. ПовещенкоА. Ф. ПовещенкоЕ. А. ПокушаловА. М. КараськовВ. И. КоненковИ. И. КимА. Б. РомановНаучно-исследовательский институт клинической и экспериментальной лимфологииObjectives and Methods. It is shown that long-term cryopreservation for 2 and 4 years leads to a reduction in the number and viability of the mononuclear cells obtained after mobilization granulocyte colony-stimulating factor (G-CSF) from patients with chronic heart failure. In addition, during prolonged cryopreservation decreased amount of content endothelial progenitor cells of the mononuclear cells from patients with chronic heart failure. Results. It was established that in cryopreservation for 2 and 4 years leads to a decreased proliferative activity of the mononuclear cells mobilized by G-CSF, both spontaneous and mitogen stimulated, increase cell number in the stage of apoptosis. The findings suggest that the negative impact on the long cryopreservation mononuclear cells obtained after G-CSF mobilization and effective cell therapy for heart failure patients need to use cells in the cryopreservation of not more than 2 years.http://journalmeshalkin.ru/index.php/heartjournal/article/view/12КРИОКОНСЕРВАЦИЯМОНОНУКЛЕАРНЫЕ КЛЕТКИЭНДОТЕЛИАЛЬНЫЕ ПРОГЕНИТОРНЫЕ КЛЕТКИХРОНИЧЕСКАЯ СЕРДЕЧНАЯ НЕДОСТАТОЧНОСТЬ |
| spellingShingle | Н. А. Бондаренко А. П. Лыков О. В. Повещенко А. Ф. Повещенко Е. А. Покушалов А. М. Караськов В. И. Коненков И. И. Ким А. Б. Романов Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure Патология кровообращения и кардиохирургия КРИОКОНСЕРВАЦИЯ МОНОНУКЛЕАРНЫЕ КЛЕТКИ ЭНДОТЕЛИАЛЬНЫЕ ПРОГЕНИТОРНЫЕ КЛЕТКИ ХРОНИЧЕСКАЯ СЕРДЕЧНАЯ НЕДОСТАТОЧНОСТЬ |
| title | Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure |
| title_full | Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure |
| title_fullStr | Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure |
| title_full_unstemmed | Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure |
| title_short | Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure |
| title_sort | influence of prolonged cryopreservation on the number and functional activity of stem progenitor cells derived after g csf mobilization from patients with chronic heart failure |
| topic | КРИОКОНСЕРВАЦИЯ МОНОНУКЛЕАРНЫЕ КЛЕТКИ ЭНДОТЕЛИАЛЬНЫЕ ПРОГЕНИТОРНЫЕ КЛЕТКИ ХРОНИЧЕСКАЯ СЕРДЕЧНАЯ НЕДОСТАТОЧНОСТЬ |
| url | http://journalmeshalkin.ru/index.php/heartjournal/article/view/12 |
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