Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid
Quercetin-3-glucuronide (Q3GA), the main phase II metabolite of quercetin (Q) in human plasma, is considered to be a more stable form of Q for transport with the bloodstream to tissues, where it can be potentially deconjugated by β-glucuronidase (β-Gluc) to Q aglycone, which easily enters the brain....
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2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| author | Małgorzata Domżalska Wiesław Wiczkowski Aleksandra Szczepkowska Sylwia Chojnowska Tomasz Misztal Fruzsina R. Walter Maria A. Deli Hiroshi Ishikawa Horst Schroten Christian Schwerk Janina Skipor |
| author_facet | Małgorzata Domżalska Wiesław Wiczkowski Aleksandra Szczepkowska Sylwia Chojnowska Tomasz Misztal Fruzsina R. Walter Maria A. Deli Hiroshi Ishikawa Horst Schroten Christian Schwerk Janina Skipor |
| author_sort | Małgorzata Domżalska |
| collection | DOAJ |
| description | Quercetin-3-glucuronide (Q3GA), the main phase II metabolite of quercetin (Q) in human plasma, is considered to be a more stable form of Q for transport with the bloodstream to tissues, where it can be potentially deconjugated by β-glucuronidase (β-Gluc) to Q aglycone, which easily enters the brain. This study evaluates the effect of lipopolysaccharide (LPS)-induced acute inflammation on β-Gluc gene expression in the choroid plexus (ChP) and its activity in blood plasma, ChP and cerebrospinal fluid (CSF), and the concentration of Q and its phase II metabolites in blood plasma and CSF. Studies were performed on saline- and LPS-treated adult ewes (<i>n</i> = 40) receiving Q3GA intravenously (<i>n</i> = 16) and on primary rat ChP epithelial cells and human ChP epithelial papilloma cells. We observed that acute inflammation stimulated β-Gluc activity in the ChP and blood plasma, but not in ChP epithelial cells and CSF, and did not affect Q and its phase II metabolite concentrations in plasma and CSF, except Q3GA, for which the plasma concentration was higher 30 min after administration (<i>p</i> < 0.05) in LPS- compared to saline-treated ewes. The lack of Q3GA deconjugation in the ChP observed under physiological and acute inflammatory conditions, however, does not exclude its possible role in the course of neurodegenerative diseases. |
| first_indexed | 2024-03-10T09:51:58Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:51:58Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9c6fb7c1605b49009249554dc1ced99b2023-11-22T02:40:36ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012213712210.3390/ijms22137122Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal FluidMałgorzata Domżalska0Wiesław Wiczkowski1Aleksandra Szczepkowska2Sylwia Chojnowska3Tomasz Misztal4Fruzsina R. Walter5Maria A. Deli6Hiroshi Ishikawa7Horst Schroten8Christian Schwerk9Janina Skipor10Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandFaculty of Health Sciences, Lomza State University of Applied Sciences, 18-400 Lomza, PolandThe Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, PolandInstitute of Biophysics, Biological Research Centre, ELKH, 6726 Szeged, HungaryInstitute of Biophysics, Biological Research Centre, ELKH, 6726 Szeged, HungaryLaboratory of Regenerative Medicine, Department of Neurosurgery, University of Tsukuba, Tsukuba 305-8575, Ibaraki, JapanDepartment of Pediatrics, Pediatric Infectious Diseases, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyDepartment of Pediatrics, Pediatric Infectious Diseases, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyInstitute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, PolandQuercetin-3-glucuronide (Q3GA), the main phase II metabolite of quercetin (Q) in human plasma, is considered to be a more stable form of Q for transport with the bloodstream to tissues, where it can be potentially deconjugated by β-glucuronidase (β-Gluc) to Q aglycone, which easily enters the brain. This study evaluates the effect of lipopolysaccharide (LPS)-induced acute inflammation on β-Gluc gene expression in the choroid plexus (ChP) and its activity in blood plasma, ChP and cerebrospinal fluid (CSF), and the concentration of Q and its phase II metabolites in blood plasma and CSF. Studies were performed on saline- and LPS-treated adult ewes (<i>n</i> = 40) receiving Q3GA intravenously (<i>n</i> = 16) and on primary rat ChP epithelial cells and human ChP epithelial papilloma cells. We observed that acute inflammation stimulated β-Gluc activity in the ChP and blood plasma, but not in ChP epithelial cells and CSF, and did not affect Q and its phase II metabolite concentrations in plasma and CSF, except Q3GA, for which the plasma concentration was higher 30 min after administration (<i>p</i> < 0.05) in LPS- compared to saline-treated ewes. The lack of Q3GA deconjugation in the ChP observed under physiological and acute inflammatory conditions, however, does not exclude its possible role in the course of neurodegenerative diseases.https://www.mdpi.com/1422-0067/22/13/7122quercetin-3-glucuronidequercetinβ-glucuronidase activitychoroid plexuschoroid plexus epithelial cellscerebrospinal fluid |
| spellingShingle | Małgorzata Domżalska Wiesław Wiczkowski Aleksandra Szczepkowska Sylwia Chojnowska Tomasz Misztal Fruzsina R. Walter Maria A. Deli Hiroshi Ishikawa Horst Schroten Christian Schwerk Janina Skipor Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid International Journal of Molecular Sciences quercetin-3-glucuronide quercetin β-glucuronidase activity choroid plexus choroid plexus epithelial cells cerebrospinal fluid |
| title | Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid |
| title_full | Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid |
| title_fullStr | Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid |
| title_full_unstemmed | Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid |
| title_short | Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid |
| title_sort | effect of lipopolysaccharide induced inflammatory challenge on β glucuronidase activity and the concentration of quercetin and its metabolites in the choroid plexus blood plasma and cerebrospinal fluid |
| topic | quercetin-3-glucuronide quercetin β-glucuronidase activity choroid plexus choroid plexus epithelial cells cerebrospinal fluid |
| url | https://www.mdpi.com/1422-0067/22/13/7122 |
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