Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass
Efficient utilization of plant biomass by enzymatic hydrolysis is currently studied worldwide but still faces enormous challenges because of the inability to break down lignocellulosic materials with high sugar yields and low enzyme dosage. Therefore, the synergistic action between various enzymes p...
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North Carolina State University
2016-11-01
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| Series: | BioResources |
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| Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_434_Wu_Heterologous_Expression_New_Acetyl |
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| author | Hongli Wu Yong Xue Hailong Li Lihui Gan Jian Liu Minnan Long |
| author_facet | Hongli Wu Yong Xue Hailong Li Lihui Gan Jian Liu Minnan Long |
| author_sort | Hongli Wu |
| collection | DOAJ |
| description | Efficient utilization of plant biomass by enzymatic hydrolysis is currently studied worldwide but still faces enormous challenges because of the inability to break down lignocellulosic materials with high sugar yields and low enzyme dosage. Therefore, the synergistic action between various enzymes plays an important role in reaching this goal. The synergistic cooperation between a novel acetyl xylan esterase (heterologous expressed at high levels in this study) and four other xylan-degrading enzymes (reported previously) were performed in this study. The acetyl xylan esterase (AnAxe) gene was cloned from Aspergillus niger BE-2 and expressed in Pichia pastoris GS115. The deduced amino acid (aa) sequence consisted of 304-aa and included a 23-aa signal peptide and 281-aa mature protein. The AnAxe was extracellularly expressed with a molecular weight of ca. 31 kDa. The purified AnAxe exhibited maximal specific activity of 480.2 IU/mg at pH 7.0 and 40 C and was still thermostable below 50 C. The metal ions used in this study and EDTA showed a slight effect on the AnAxe. A significant synergistic effect was determined between AnAxe and the other four xylan-degrading enzymes, including endo--1,4-xylanases, -xylosidases, -L-arabinofurano-sidases, and -glucuronidases, on the degradation of bamboo biomass. The highest degree of synergism was obtained between AnAxe and endo--1,4-xylanases/-xylosidases. |
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| issn | 1930-2126 1930-2126 |
| language | English |
| last_indexed | 2024-12-23T05:30:22Z |
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| series | BioResources |
| spelling | doaj.art-9c711b64e03147b6951a7ab757adb86b2022-12-21T17:58:29ZengNorth Carolina State UniversityBioResources1930-21261930-21262016-11-0112143444710.15376/biores.12.1.434-447Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo BiomassHongli Wu0Yong Xue1Hailong Li2Lihui Gan3Jian Liu4Minnan Long5College of Energy, Xiamen University; ChinaCollege of Energy, Xiamen University, Xiamen 361005, P. R. China; ChinaKey Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences; ChinaCollege of Energy, Xiamen University; ChinaCollege of Energy, Xiamen University; ChinaCollege of Energy, Xiamen University; ChinaEfficient utilization of plant biomass by enzymatic hydrolysis is currently studied worldwide but still faces enormous challenges because of the inability to break down lignocellulosic materials with high sugar yields and low enzyme dosage. Therefore, the synergistic action between various enzymes plays an important role in reaching this goal. The synergistic cooperation between a novel acetyl xylan esterase (heterologous expressed at high levels in this study) and four other xylan-degrading enzymes (reported previously) were performed in this study. The acetyl xylan esterase (AnAxe) gene was cloned from Aspergillus niger BE-2 and expressed in Pichia pastoris GS115. The deduced amino acid (aa) sequence consisted of 304-aa and included a 23-aa signal peptide and 281-aa mature protein. The AnAxe was extracellularly expressed with a molecular weight of ca. 31 kDa. The purified AnAxe exhibited maximal specific activity of 480.2 IU/mg at pH 7.0 and 40 C and was still thermostable below 50 C. The metal ions used in this study and EDTA showed a slight effect on the AnAxe. A significant synergistic effect was determined between AnAxe and the other four xylan-degrading enzymes, including endo--1,4-xylanases, -xylosidases, -L-arabinofurano-sidases, and -glucuronidases, on the degradation of bamboo biomass. The highest degree of synergism was obtained between AnAxe and endo--1,4-xylanases/-xylosidases.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_434_Wu_Heterologous_Expression_New_AcetylAspergillus niger; Acetyl xylan esterase; Pichia pastoris; Characterization; Synergistic cooperation |
| spellingShingle | Hongli Wu Yong Xue Hailong Li Lihui Gan Jian Liu Minnan Long Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass BioResources Aspergillus niger; Acetyl xylan esterase; Pichia pastoris; Characterization; Synergistic cooperation |
| title | Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass |
| title_full | Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass |
| title_fullStr | Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass |
| title_full_unstemmed | Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass |
| title_short | Heterologous Expression of a New Acetyl Xylan Esterase from Aspergillus niger BE-2 and its Synergistic Action with Xylan-Degrading Enzymes in the Hydrolysis of Bamboo Biomass |
| title_sort | heterologous expression of a new acetyl xylan esterase from aspergillus niger be 2 and its synergistic action with xylan degrading enzymes in the hydrolysis of bamboo biomass |
| topic | Aspergillus niger; Acetyl xylan esterase; Pichia pastoris; Characterization; Synergistic cooperation |
| url | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_12_1_434_Wu_Heterologous_Expression_New_Acetyl |
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