Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection
In this study, we describe a novel peroxidase-like activity of Co-aminoclay [CoAC] present at pH ~5.0 and its application to fluorescent biosensor for the determination of H2O2 and glucose. It is synthesized with aminoclays (ACs) entrapping cationic metals such as Fe, Cu, Al, Co., Ce, Ni, Mn, and Zn...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-02-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/2/457 |
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| author | Han Pill Song Yongil Lee Vu Khac Hoang Bui You-Kwon Oh Hyun Gyu Park Moon Il Kim Young-Chul Lee |
| author_facet | Han Pill Song Yongil Lee Vu Khac Hoang Bui You-Kwon Oh Hyun Gyu Park Moon Il Kim Young-Chul Lee |
| author_sort | Han Pill Song |
| collection | DOAJ |
| description | In this study, we describe a novel peroxidase-like activity of Co-aminoclay [CoAC] present at pH ~5.0 and its application to fluorescent biosensor for the determination of H2O2 and glucose. It is synthesized with aminoclays (ACs) entrapping cationic metals such as Fe, Cu, Al, Co., Ce, Ni, Mn, and Zn to find enzyme mimicking ACs by sol–gel ambient conditions. Through the screening of catalytic activities by the typical colorimetric reaction employing 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid)diammonium salt (ABTS) as a substrate with or without H2O2, Fe, Cu, and CoACs are found to exhibit peroxidase-like activity, as well as oxidase-like activity was observed from Ce and MnACs. Among them, CoAC shows exceptionally high peroxidase-like activity, presumably due to its ability to induce electron transfer between substrates and H2O2. CoAC is then used to catalyze the oxidation of Amplex® UltraRed (AUR) into a fluorescent end product, which enables a sensitive fluorescent detection of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy is developed, based on enzyme cascade reaction between glucose oxidase (GOx) and CoAC. Using this strategy, a highly linear fluorescence enhancement is verified when the concentration of glucose is increased in a wide range from 10 μM to 1 mM with a lower detection limit of 5 μM. The practical diagnostic capability of the assay system is also verified by its use to detect glucose in human blood serum. Based on these results, it is anticipated that CoAC can serve as potent peroxidase mimetics for the detection of clinically important target molecules. |
| first_indexed | 2024-04-11T22:18:27Z |
| format | Article |
| id | doaj.art-9c71a304add8464fa639aed8912adf22 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:18:27Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-9c71a304add8464fa639aed8912adf222022-12-22T04:00:18ZengMDPI AGSensors1424-82202018-02-0118245710.3390/s18020457s18020457Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose DetectionHan Pill Song0Yongil Lee1Vu Khac Hoang Bui2You-Kwon Oh3Hyun Gyu Park4Moon Il Kim5Young-Chul Lee6Department of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, KoreaKorea Railroad Research Institute (KRRI), 176 Cheoldobakmulkwan-ro, Uiwang-si, Gyeonggi-do 16105, KoreaDepartment of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, KoreaSchool of Chemical and Biomolecular Engineering, Pusan National University, Busan 46241, KoreaDepartment of Chemical and Biomolecular Engineering (BK21+ Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, KoreaDepartment of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, KoreaDepartment of BioNano Technology, Gachon University, 1342 Seongnamdae-ro, Sujeong-gu, Seongnam-Si, Gyeonggi-do 13120, KoreaIn this study, we describe a novel peroxidase-like activity of Co-aminoclay [CoAC] present at pH ~5.0 and its application to fluorescent biosensor for the determination of H2O2 and glucose. It is synthesized with aminoclays (ACs) entrapping cationic metals such as Fe, Cu, Al, Co., Ce, Ni, Mn, and Zn to find enzyme mimicking ACs by sol–gel ambient conditions. Through the screening of catalytic activities by the typical colorimetric reaction employing 2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid)diammonium salt (ABTS) as a substrate with or without H2O2, Fe, Cu, and CoACs are found to exhibit peroxidase-like activity, as well as oxidase-like activity was observed from Ce and MnACs. Among them, CoAC shows exceptionally high peroxidase-like activity, presumably due to its ability to induce electron transfer between substrates and H2O2. CoAC is then used to catalyze the oxidation of Amplex® UltraRed (AUR) into a fluorescent end product, which enables a sensitive fluorescent detection of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy is developed, based on enzyme cascade reaction between glucose oxidase (GOx) and CoAC. Using this strategy, a highly linear fluorescence enhancement is verified when the concentration of glucose is increased in a wide range from 10 μM to 1 mM with a lower detection limit of 5 μM. The practical diagnostic capability of the assay system is also verified by its use to detect glucose in human blood serum. Based on these results, it is anticipated that CoAC can serve as potent peroxidase mimetics for the detection of clinically important target molecules.http://www.mdpi.com/1424-8220/18/2/457Co-aminoclay [CoAC]peroxidase mimeticfluorescent biosensorcolorimetric sensorglucose detection |
| spellingShingle | Han Pill Song Yongil Lee Vu Khac Hoang Bui You-Kwon Oh Hyun Gyu Park Moon Il Kim Young-Chul Lee Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection Sensors Co-aminoclay [CoAC] peroxidase mimetic fluorescent biosensor colorimetric sensor glucose detection |
| title | Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection |
| title_full | Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection |
| title_fullStr | Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection |
| title_full_unstemmed | Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection |
| title_short | Effective Peroxidase-Like Activity of Co-Aminoclay [CoAC] and Its Application for Glucose Detection |
| title_sort | effective peroxidase like activity of co aminoclay coac and its application for glucose detection |
| topic | Co-aminoclay [CoAC] peroxidase mimetic fluorescent biosensor colorimetric sensor glucose detection |
| url | http://www.mdpi.com/1424-8220/18/2/457 |
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