Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissue
Nanoparticles (NPs), materials that have one dimension less than 100 nm, are used in manufacturing, health and food products, and consumer products including cosmetics, clothing and household appliances. Their utility to industry is derived from their high surface-area-to-volume ratios and physico-c...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-11-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2013.00251/full |
| _version_ | 1818790488696684544 |
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| author | S. Austin eHammond Amanda eCarew Caren eHelbing |
| author_facet | S. Austin eHammond Amanda eCarew Caren eHelbing |
| author_sort | S. Austin eHammond |
| collection | DOAJ |
| description | Nanoparticles (NPs), materials that have one dimension less than 100 nm, are used in manufacturing, health and food products, and consumer products including cosmetics, clothing and household appliances. Their utility to industry is derived from their high surface-area-to-volume ratios and physico-chemical properties distinct from their bulk counterparts, but the near-certainty that NPs will be released into the environment raises the possibility that they could present health risks to humans and wildlife. The thyroid hormones (THs), thyroxine and 3,3’,5-triiodothyronine (T3), are involved in development and metabolism in vertebrates including humans and frogs. Many of the processes of anuran metamorphosis are analogous to human post-embryonic development and disruption of TH action can have drastic effects. These shared features make the metamorphosis of anurans an excellent model for screening for endocrine disrupting chemicals (EDCs). We used the cultured tailfin (C-fin) assay to examine the exposure effects of 0.1-10 nM (~8-800 ng/L) of three types of ~20 nm TiO2 NPs (P25, M212, M262) and micron-sized TiO2 (μTiO2) ±10 nM T3. The actual Ti levels were 40.9 – 64.7% of the nominal value. Real-time quantitative polymerase chain reaction (QPCR) was used to measure the relative amounts of mRNA transcripts encoding TH-responsive thyroid hormone receptors (thra and thrb) and Rana larval keratin type I (rlk1), as well as the cellular stress-responsive heat shock protein 30 kDa (hsp30), superoxide dismutase (sod), and catalase (cat). The levels of the TH-responsive transcripts were largely unaffected by any form of TiO2. Some significant effects on stress-related transcripts were observed upon exposure to micron-sized TiO2, P25 and M212 while no effect was observed with M262 exposure. Therefore the risk of adversely affecting amphibian tissue by disrupting TH-signalling or inducing cellular stress is low for these compounds relative to other previously-tested NPs. |
| first_indexed | 2024-12-18T14:56:15Z |
| format | Article |
| id | doaj.art-9c71c4cef11c4407a8113f5f72ba5c95 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-18T14:56:15Z |
| publishDate | 2013-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-9c71c4cef11c4407a8113f5f72ba5c952022-12-21T21:04:02ZengFrontiers Media S.A.Frontiers in Genetics1664-80212013-11-01410.3389/fgene.2013.0025158046Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissueS. Austin eHammond0Amanda eCarew1Caren eHelbing2University of VictoriaUniversity of VictoriaUniversity of VictoriaNanoparticles (NPs), materials that have one dimension less than 100 nm, are used in manufacturing, health and food products, and consumer products including cosmetics, clothing and household appliances. Their utility to industry is derived from their high surface-area-to-volume ratios and physico-chemical properties distinct from their bulk counterparts, but the near-certainty that NPs will be released into the environment raises the possibility that they could present health risks to humans and wildlife. The thyroid hormones (THs), thyroxine and 3,3’,5-triiodothyronine (T3), are involved in development and metabolism in vertebrates including humans and frogs. Many of the processes of anuran metamorphosis are analogous to human post-embryonic development and disruption of TH action can have drastic effects. These shared features make the metamorphosis of anurans an excellent model for screening for endocrine disrupting chemicals (EDCs). We used the cultured tailfin (C-fin) assay to examine the exposure effects of 0.1-10 nM (~8-800 ng/L) of three types of ~20 nm TiO2 NPs (P25, M212, M262) and micron-sized TiO2 (μTiO2) ±10 nM T3. The actual Ti levels were 40.9 – 64.7% of the nominal value. Real-time quantitative polymerase chain reaction (QPCR) was used to measure the relative amounts of mRNA transcripts encoding TH-responsive thyroid hormone receptors (thra and thrb) and Rana larval keratin type I (rlk1), as well as the cellular stress-responsive heat shock protein 30 kDa (hsp30), superoxide dismutase (sod), and catalase (cat). The levels of the TH-responsive transcripts were largely unaffected by any form of TiO2. Some significant effects on stress-related transcripts were observed upon exposure to micron-sized TiO2, P25 and M212 while no effect was observed with M262 exposure. Therefore the risk of adversely affecting amphibian tissue by disrupting TH-signalling or inducing cellular stress is low for these compounds relative to other previously-tested NPs.http://journal.frontiersin.org/Journal/10.3389/fgene.2013.00251/fullOxidative StressamphibiannanoparticleThyroid hormoneorgan culturetitanium dioxide |
| spellingShingle | S. Austin eHammond Amanda eCarew Caren eHelbing Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissue Frontiers in Genetics Oxidative Stress amphibian nanoparticle Thyroid hormone organ culture titanium dioxide |
| title | Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissue |
| title_full | Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissue |
| title_fullStr | Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissue |
| title_full_unstemmed | Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissue |
| title_short | Evaluation of the effects of titanium dioxide nanoparticles on cultured Rana catesbeiana tailfin tissue |
| title_sort | evaluation of the effects of titanium dioxide nanoparticles on cultured rana catesbeiana tailfin tissue |
| topic | Oxidative Stress amphibian nanoparticle Thyroid hormone organ culture titanium dioxide |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2013.00251/full |
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