Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis?
Matrix Metaloproteinase-9 (MMP-9) and Tissue Inhibitor of Metaloproteinase-1 (TIMP-1), enzymes involved in tissue remodelling, have been previously reported to be overexpressed in the colonic mucosa of patients with Ulcerative colitis (UC). The aim of this study was to determine the relation of MMP-...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/11/9/2655 |
| _version_ | 1797504156728033280 |
|---|---|
| author | Aleksandra Czajkowska Katarzyna Guzinska-Ustymowicz Anna Pryczynicz Dariusz Lebensztejn Urszula Daniluk |
| author_facet | Aleksandra Czajkowska Katarzyna Guzinska-Ustymowicz Anna Pryczynicz Dariusz Lebensztejn Urszula Daniluk |
| author_sort | Aleksandra Czajkowska |
| collection | DOAJ |
| description | Matrix Metaloproteinase-9 (MMP-9) and Tissue Inhibitor of Metaloproteinase-1 (TIMP-1), enzymes involved in tissue remodelling, have been previously reported to be overexpressed in the colonic mucosa of patients with Ulcerative colitis (UC). The aim of this study was to determine the relation of MMP-9 and TIMP-1 with UC phenotypes, the disease activity index and routinely tested inflammatory markers in newly diagnosed paediatric patients. The study group comprised 35 children diagnosed with UC and 20 control groups. Serum and faecal concentrations of MMP-9 and TIMP-1 were estimated using enzyme-like immunosorbent assay kits and correlated to the disease activity index (Paediatric Ulcerative Colitis Activity Index, PUCAI), UC phenotype (Paris Classification), inflammatory markers and endoscopic score (Mayo score). Children with UC presented with significantly higher serum and faecal concentrations of studied markers compared to the control group. Both serums, MMP-9 and TIMP-1, were higher in children with more extended and severe lesions in the colon. Furthermore, serum MMP-9 correlated with the Mayo score, Paris classification and C-reactive protein (CRP) levels. Serum TIMP-1 showed correlation with PUCAI, Paris Classification, CRP levels and the erythrocyte sedimentation rate. Serum and faecal levels of MMP-9 and TIMP-1 are useful in discriminating UC patients and non-invasive assessments of disease phenotypes. It seemed that simultaneous measurement of these proteins in combination with other common markers of inflammation could be applied in clinical practice. |
| first_indexed | 2024-03-10T04:00:39Z |
| format | Article |
| id | doaj.art-9c77b97843e7430ea3274921ccb3dfb5 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T04:00:39Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-9c77b97843e7430ea3274921ccb3dfb52023-11-23T08:35:33ZengMDPI AGJournal of Clinical Medicine2077-03832022-05-01119265510.3390/jcm11092655Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis?Aleksandra Czajkowska0Katarzyna Guzinska-Ustymowicz1Anna Pryczynicz2Dariusz Lebensztejn3Urszula Daniluk4Department of Pediatrics, Gastroenterology, Hepatology, Nutrition and Allergology, Medical University of Bialystok, 17 Waszyngtona Street, 15-274 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, 15-089 Bialystok, PolandDepartment of General Pathomorphology, Medical University of Bialystok, 15-089 Bialystok, PolandDepartment of Pediatrics, Gastroenterology, Hepatology, Nutrition and Allergology, Medical University of Bialystok, 17 Waszyngtona Street, 15-274 Bialystok, PolandDepartment of Pediatrics, Gastroenterology, Hepatology, Nutrition and Allergology, Medical University of Bialystok, 17 Waszyngtona Street, 15-274 Bialystok, PolandMatrix Metaloproteinase-9 (MMP-9) and Tissue Inhibitor of Metaloproteinase-1 (TIMP-1), enzymes involved in tissue remodelling, have been previously reported to be overexpressed in the colonic mucosa of patients with Ulcerative colitis (UC). The aim of this study was to determine the relation of MMP-9 and TIMP-1 with UC phenotypes, the disease activity index and routinely tested inflammatory markers in newly diagnosed paediatric patients. The study group comprised 35 children diagnosed with UC and 20 control groups. Serum and faecal concentrations of MMP-9 and TIMP-1 were estimated using enzyme-like immunosorbent assay kits and correlated to the disease activity index (Paediatric Ulcerative Colitis Activity Index, PUCAI), UC phenotype (Paris Classification), inflammatory markers and endoscopic score (Mayo score). Children with UC presented with significantly higher serum and faecal concentrations of studied markers compared to the control group. Both serums, MMP-9 and TIMP-1, were higher in children with more extended and severe lesions in the colon. Furthermore, serum MMP-9 correlated with the Mayo score, Paris classification and C-reactive protein (CRP) levels. Serum TIMP-1 showed correlation with PUCAI, Paris Classification, CRP levels and the erythrocyte sedimentation rate. Serum and faecal levels of MMP-9 and TIMP-1 are useful in discriminating UC patients and non-invasive assessments of disease phenotypes. It seemed that simultaneous measurement of these proteins in combination with other common markers of inflammation could be applied in clinical practice.https://www.mdpi.com/2077-0383/11/9/2655ulcerative colitismetalloproteinaseTissue Inhibitor of Metaloproteinasechildren |
| spellingShingle | Aleksandra Czajkowska Katarzyna Guzinska-Ustymowicz Anna Pryczynicz Dariusz Lebensztejn Urszula Daniluk Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis? Journal of Clinical Medicine ulcerative colitis metalloproteinase Tissue Inhibitor of Metaloproteinase children |
| title | Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis? |
| title_full | Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis? |
| title_fullStr | Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis? |
| title_full_unstemmed | Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis? |
| title_short | Are Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 Useful as Markers in Diagnostic Management of Children with Newly Diagnosed Ulcerative Colitis? |
| title_sort | are matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 useful as markers in diagnostic management of children with newly diagnosed ulcerative colitis |
| topic | ulcerative colitis metalloproteinase Tissue Inhibitor of Metaloproteinase children |
| url | https://www.mdpi.com/2077-0383/11/9/2655 |
| work_keys_str_mv | AT aleksandraczajkowska arematrixmetalloproteinase9andtissueinhibitorofmetalloproteinase1usefulasmarkersindiagnosticmanagementofchildrenwithnewlydiagnosedulcerativecolitis AT katarzynaguzinskaustymowicz arematrixmetalloproteinase9andtissueinhibitorofmetalloproteinase1usefulasmarkersindiagnosticmanagementofchildrenwithnewlydiagnosedulcerativecolitis AT annapryczynicz arematrixmetalloproteinase9andtissueinhibitorofmetalloproteinase1usefulasmarkersindiagnosticmanagementofchildrenwithnewlydiagnosedulcerativecolitis AT dariuszlebensztejn arematrixmetalloproteinase9andtissueinhibitorofmetalloproteinase1usefulasmarkersindiagnosticmanagementofchildrenwithnewlydiagnosedulcerativecolitis AT urszuladaniluk arematrixmetalloproteinase9andtissueinhibitorofmetalloproteinase1usefulasmarkersindiagnosticmanagementofchildrenwithnewlydiagnosedulcerativecolitis |