Evaluating the function of murine quiescent hematopoietic stem cells following non-homologous end joining-based genome editing

Evaluating the function of murine quiescent hematopoietic stem cells following non-homologous end joining-based genome editing

Summary: Preculture is indispensable for achieving highly efficient non-homologous end joining (NHEJ)-based genome editing. Here, we present a protocol for optimizing genome editing conditions for murine hematopoietic stem cells (HSCs) and evaluating their function following NHEJ-based genome editin...

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Bibliographic Details
Main Authors: Kohei Shiroshita, Hiroshi Kobayashi, Keiyo Takubo
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
CRISPR
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723003143

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