Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses
Mycobacterium bovis is a bacterium belonging to the Mycobacterium tuberculosis complex that causes tuberculosis in cattle and in other domestic and wild animals, as well as in humans. Disease control measures are carried out by slaughtering animals tested positive in the intradermal tuberculinizatio...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2022-02-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44411 |
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| author | Flávio Gomes Barcelos Taís Ramalho dos Anjos Leila Auxiliadora de Arruda Alencar Vinicius Silva Castro Adelino Cunha-Neto Fernanda Tavares Carvalho Mariano Martinez Espinosa Ricardo Cesar Tavares Carvalho Eduardo Eustáquio de Souza Figueiredo |
| author_facet | Flávio Gomes Barcelos Taís Ramalho dos Anjos Leila Auxiliadora de Arruda Alencar Vinicius Silva Castro Adelino Cunha-Neto Fernanda Tavares Carvalho Mariano Martinez Espinosa Ricardo Cesar Tavares Carvalho Eduardo Eustáquio de Souza Figueiredo |
| author_sort | Flávio Gomes Barcelos |
| collection | DOAJ |
| description | Mycobacterium bovis is a bacterium belonging to the Mycobacterium tuberculosis complex that causes tuberculosis in cattle and in other domestic and wild animals, as well as in humans. Disease control measures are carried out by slaughtering animals tested positive in the intradermal tuberculinization test and sanitation of their original living spaces, in addition to epidemiological surveillance carried out through the sanitary inspection of bovine carcasses in slaughterhouses. In the latter, official inspection services collect samples from macroscopic lesions suspected of bovine tuberculosis, which are then sent for laboratory analysis. Knowledge concerning the variables associated with the occurrence of M. bovis can aid in decision-making regarding control and disease eradication efforts. In this context, the aim of this study was to identify the risk factors for a positive M. bovis diagnosis in suspected bovine tuberculosis lesions obtained during epidemiological surveillance activities in the state of Mato Grosso, Brazil. A total of 105 suspicious lesions were analyzed using the Nested Polymerase Chain Reaction (nested q-PCR) method, of which 14 (13.33%) tested positive for M. bovis. Univariate and bivariate statistical analyses indicated that the variable “animal slaughter” was the only risk factor presenting statistical significance associated with the diagnosis of M. bovis (p < 0.05), demonstrating that macroscopic lesions suspected as being caused by bovine tuberculosis from animals with an in vivo diagnosis were 2.82 - fold more likely to result in a positive M. bovis diagnosis by molecular tests. |
| first_indexed | 2024-04-10T20:38:35Z |
| format | Article |
| id | doaj.art-9c7ce0aa6bd248ea9154cb351de7aea9 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-04-10T20:38:35Z |
| publishDate | 2022-02-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-9c7ce0aa6bd248ea9154cb351de7aea92023-01-24T20:28:58ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592022-02-0143210.5433/1679-0359.2022v43n2p713Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhousesFlávio Gomes Barcelos0Taís Ramalho dos Anjos1Leila Auxiliadora de Arruda Alencar2Vinicius Silva Castro3Adelino Cunha-Neto4Fernanda Tavares Carvalho5Mariano Martinez Espinosa6Ricardo Cesar Tavares Carvalho7Eduardo Eustáquio de Souza Figueiredo8Universidade Federal de Mato GrossoUniversidade de CuiabáUniversidade Federal de Mato GrossoUniversidade Federal de Mato GrossoUniversidade Federal de Mato GrossoUniversidade Federal de Mato GrossoUniversidade Federal de Mato GrossoUniversidade Federal de Mato GrossoUniversidade Federal de Mato GrossoMycobacterium bovis is a bacterium belonging to the Mycobacterium tuberculosis complex that causes tuberculosis in cattle and in other domestic and wild animals, as well as in humans. Disease control measures are carried out by slaughtering animals tested positive in the intradermal tuberculinization test and sanitation of their original living spaces, in addition to epidemiological surveillance carried out through the sanitary inspection of bovine carcasses in slaughterhouses. In the latter, official inspection services collect samples from macroscopic lesions suspected of bovine tuberculosis, which are then sent for laboratory analysis. Knowledge concerning the variables associated with the occurrence of M. bovis can aid in decision-making regarding control and disease eradication efforts. In this context, the aim of this study was to identify the risk factors for a positive M. bovis diagnosis in suspected bovine tuberculosis lesions obtained during epidemiological surveillance activities in the state of Mato Grosso, Brazil. A total of 105 suspicious lesions were analyzed using the Nested Polymerase Chain Reaction (nested q-PCR) method, of which 14 (13.33%) tested positive for M. bovis. Univariate and bivariate statistical analyses indicated that the variable “animal slaughter” was the only risk factor presenting statistical significance associated with the diagnosis of M. bovis (p < 0.05), demonstrating that macroscopic lesions suspected as being caused by bovine tuberculosis from animals with an in vivo diagnosis were 2.82 - fold more likely to result in a positive M. bovis diagnosis by molecular tests.https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44411Complex Mycobacterium tuberculosisNested q-PCRPost mortem inspectionBeef. |
| spellingShingle | Flávio Gomes Barcelos Taís Ramalho dos Anjos Leila Auxiliadora de Arruda Alencar Vinicius Silva Castro Adelino Cunha-Neto Fernanda Tavares Carvalho Mariano Martinez Espinosa Ricardo Cesar Tavares Carvalho Eduardo Eustáquio de Souza Figueiredo Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses Semina: Ciências Agrárias Complex Mycobacterium tuberculosis Nested q-PCR Post mortem inspection Beef. |
| title | Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses |
| title_full | Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses |
| title_fullStr | Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses |
| title_full_unstemmed | Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses |
| title_short | Risk factors associated with the presence of Mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses |
| title_sort | risk factors associated with the presence of mycobacterium bovis in macroscopic lesions suspected as being caused by bovine tuberculosis detected in slaughterhouses |
| topic | Complex Mycobacterium tuberculosis Nested q-PCR Post mortem inspection Beef. |
| url | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/44411 |
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