A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural study
Carcinosarcoma of the anus is rare and has yet to be reportedly associated with the keratinocyte-specific Human Papilloma Virus (HPV). We describe a case of anal carcinosarcoma with HPV infection in both the epithelial and mesenchymal components of the tumor by immunohistochemistry, chromogenic in-s...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-12-01
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| Series: | Papillomavirus Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405852116300362 |
| _version_ | 1811310005881667584 |
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| author | Richard A. Hickman Azore-Dee Bradshaw Nicholas Cassai Antonio Galvao Neto David Zhou Tinghao Fu Peng Lee Zhiheng Pei Rosemary Wieczorek |
| author_facet | Richard A. Hickman Azore-Dee Bradshaw Nicholas Cassai Antonio Galvao Neto David Zhou Tinghao Fu Peng Lee Zhiheng Pei Rosemary Wieczorek |
| author_sort | Richard A. Hickman |
| collection | DOAJ |
| description | Carcinosarcoma of the anus is rare and has yet to be reportedly associated with the keratinocyte-specific Human Papilloma Virus (HPV). We describe a case of anal carcinosarcoma with HPV infection in both the epithelial and mesenchymal components of the tumor by immunohistochemistry, chromogenic in-situ hybridization (CISH) and further supported by electron microscopy (EM). Microscopic examination of the tumor showed nests of poorly-differentiated invasive squamous cell carcinoma with basaloid features intermixed with a hypercellular, atypical spindle cell proliferation. Immunohistochemistry demonstrated that the epithelial component was positive for AE1/AE3, p63, CK5/6 and p16, whilst the mesenchymal component was positive for smooth muscle actin, vimentin, and focally positive for desmin and p16, consistent with carcinosarcoma. The tumor was negative for GATA-3, CK7 and CK20. CISH demonstrated that the tumor was positive for high risk HPV (subtype 16/18) in both tumor components. EM further supported the presence of intracellular virus particles (~50 nm) that is compatible with HPV infection. Infection of both epithelial and mesenchymal tumor components by HPV has not been previously observed in the gastrointestinal tract. This finding may represent initial epithelial HPV infection with subsequent divergent tumoral differentiation and suggests the presence of viral replication in both biphasic tumor components. Keywords: Carcinosarcoma, Human Papilloma Virus, Gastrointestinal |
| first_indexed | 2024-04-13T09:52:09Z |
| format | Article |
| id | doaj.art-9c7fcbc5d5ae4e169d26e66b384c0077 |
| institution | Directory Open Access Journal |
| issn | 2405-8521 |
| language | English |
| last_indexed | 2024-04-13T09:52:09Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Papillomavirus Research |
| spelling | doaj.art-9c7fcbc5d5ae4e169d26e66b384c00772022-12-22T02:51:34ZengElsevierPapillomavirus Research2405-85212016-12-012164166A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural studyRichard A. Hickman0Azore-Dee Bradshaw1Nicholas Cassai2Antonio Galvao Neto3David Zhou4Tinghao Fu5Peng Lee6Zhiheng Pei7Rosemary Wieczorek8Department of Pathology, New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USA; Corresponding author at: New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USA.Department of Pathology, New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USADepartment of Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, USADepartment of Pathology, New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USADepartment of Pathology, New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USADepartment of Pathology, New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USADepartment of Pathology, New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USA; Department of Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, USADepartment of Pathology, New York University Langone Medical Center, 550 First Avenue, New York, NY 10016, USA; Department of Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, USADepartment of Veterans Affairs New York Harbor Healthcare System, New York, NY 10010, USACarcinosarcoma of the anus is rare and has yet to be reportedly associated with the keratinocyte-specific Human Papilloma Virus (HPV). We describe a case of anal carcinosarcoma with HPV infection in both the epithelial and mesenchymal components of the tumor by immunohistochemistry, chromogenic in-situ hybridization (CISH) and further supported by electron microscopy (EM). Microscopic examination of the tumor showed nests of poorly-differentiated invasive squamous cell carcinoma with basaloid features intermixed with a hypercellular, atypical spindle cell proliferation. Immunohistochemistry demonstrated that the epithelial component was positive for AE1/AE3, p63, CK5/6 and p16, whilst the mesenchymal component was positive for smooth muscle actin, vimentin, and focally positive for desmin and p16, consistent with carcinosarcoma. The tumor was negative for GATA-3, CK7 and CK20. CISH demonstrated that the tumor was positive for high risk HPV (subtype 16/18) in both tumor components. EM further supported the presence of intracellular virus particles (~50 nm) that is compatible with HPV infection. Infection of both epithelial and mesenchymal tumor components by HPV has not been previously observed in the gastrointestinal tract. This finding may represent initial epithelial HPV infection with subsequent divergent tumoral differentiation and suggests the presence of viral replication in both biphasic tumor components. Keywords: Carcinosarcoma, Human Papilloma Virus, Gastrointestinalhttp://www.sciencedirect.com/science/article/pii/S2405852116300362 |
| spellingShingle | Richard A. Hickman Azore-Dee Bradshaw Nicholas Cassai Antonio Galvao Neto David Zhou Tinghao Fu Peng Lee Zhiheng Pei Rosemary Wieczorek A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural study Papillomavirus Research |
| title | A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural study |
| title_full | A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural study |
| title_fullStr | A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural study |
| title_full_unstemmed | A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural study |
| title_short | A rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements: An immunohistochemical, molecular and ultrastructural study |
| title_sort | rare case of anal carcinosarcoma with human papilloma virus infection in both biphasic tumor elements an immunohistochemical molecular and ultrastructural study |
| url | http://www.sciencedirect.com/science/article/pii/S2405852116300362 |
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