The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it>
<p>Abstract</p> <p>Background</p> <p>It is supposed that fibroblasts present in tumour microenvironment increase cancer invasiveness and its ability to metastasize but the mechanisms have not been clearly defined yet. Thus, the current study was designed to assess chang...
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| Format: | Article |
| Language: | English |
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BMC
2012-03-01
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| Series: | BMC Veterinary Research |
| Online Access: | http://www.biomedcentral.com/1746-6148/8/35 |
| _version_ | 1819087251766771712 |
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| author | Król Magdalena Pawłowski Karol M Szyszko Katarzyna Maciejewski Henryk Dolka Izabella Manuali Elisabetta Jank Michał Motyl Tomasz |
| author_facet | Król Magdalena Pawłowski Karol M Szyszko Katarzyna Maciejewski Henryk Dolka Izabella Manuali Elisabetta Jank Michał Motyl Tomasz |
| author_sort | Król Magdalena |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>It is supposed that fibroblasts present in tumour microenvironment increase cancer invasiveness and its ability to metastasize but the mechanisms have not been clearly defined yet. Thus, the current study was designed to assess changes in gene expression in five various cancer cell lines grown as a co-culture with the carcinoma-associated fibroblasts (CAFs) <it>in vitro</it>.</p> <p>Results</p> <p>A carcinoma-associated fibroblast cell line was isolated from a canine mammary cancer. Then, a co-culture of cancer cells with the CAFs was established and maintained for 72 hrs. Having sorted the cells, a global gene expression in cancer cells using DNA microarrays was examined. The analysis revealed an up-regulation of 100 genes and a down-regulation of 106 genes in the cancer cells grown as a co-culture with the CAFs in comparison to control conditions. The PANTHER binomial statistics tool was applied to determine statistically over-manifested pathways (<it>p </it>< 0.05). Bulk of the up-regulated genes are involved in the adhesion, the angiogenesis, the epithelial-mesenchymal transition (EMT) and generally take part in the developmental processes. These results were further confirmed using real-time qPCR. Moreover, a wound-healing assay and growth characteristics on Matrigel matrix showed that CAFs increase cancer cell migration and matrix invasion.</p> <p>Conclusion</p> <p>The results of the current study showed that the co-culturing of cancer cells and the CAFs caused significant changes to the cancer gene expression. The presence of the CAFs in a microenvironment of cancer cells promotes adhesion, angiogenesis and EMT.</p> |
| first_indexed | 2024-12-21T21:33:11Z |
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| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-21T21:33:11Z |
| publishDate | 2012-03-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj.art-9c83361851b745fcb7727ed8d0a878cd2022-12-21T18:49:34ZengBMCBMC Veterinary Research1746-61482012-03-01813510.1186/1746-6148-8-35The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it>Król MagdalenaPawłowski Karol MSzyszko KatarzynaMaciejewski HenrykDolka IzabellaManuali ElisabettaJank MichałMotyl Tomasz<p>Abstract</p> <p>Background</p> <p>It is supposed that fibroblasts present in tumour microenvironment increase cancer invasiveness and its ability to metastasize but the mechanisms have not been clearly defined yet. Thus, the current study was designed to assess changes in gene expression in five various cancer cell lines grown as a co-culture with the carcinoma-associated fibroblasts (CAFs) <it>in vitro</it>.</p> <p>Results</p> <p>A carcinoma-associated fibroblast cell line was isolated from a canine mammary cancer. Then, a co-culture of cancer cells with the CAFs was established and maintained for 72 hrs. Having sorted the cells, a global gene expression in cancer cells using DNA microarrays was examined. The analysis revealed an up-regulation of 100 genes and a down-regulation of 106 genes in the cancer cells grown as a co-culture with the CAFs in comparison to control conditions. The PANTHER binomial statistics tool was applied to determine statistically over-manifested pathways (<it>p </it>< 0.05). Bulk of the up-regulated genes are involved in the adhesion, the angiogenesis, the epithelial-mesenchymal transition (EMT) and generally take part in the developmental processes. These results were further confirmed using real-time qPCR. Moreover, a wound-healing assay and growth characteristics on Matrigel matrix showed that CAFs increase cancer cell migration and matrix invasion.</p> <p>Conclusion</p> <p>The results of the current study showed that the co-culturing of cancer cells and the CAFs caused significant changes to the cancer gene expression. The presence of the CAFs in a microenvironment of cancer cells promotes adhesion, angiogenesis and EMT.</p>http://www.biomedcentral.com/1746-6148/8/35 |
| spellingShingle | Król Magdalena Pawłowski Karol M Szyszko Katarzyna Maciejewski Henryk Dolka Izabella Manuali Elisabetta Jank Michał Motyl Tomasz The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it> BMC Veterinary Research |
| title | The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it> |
| title_full | The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it> |
| title_fullStr | The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it> |
| title_full_unstemmed | The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it> |
| title_short | The gene expression profiles of canine mammary cancer cells grown with carcinoma-associated fibroblasts (CAFs) as a co-culture <it>in vitro</it> |
| title_sort | gene expression profiles of canine mammary cancer cells grown with carcinoma associated fibroblasts cafs as a co culture it in vitro it |
| url | http://www.biomedcentral.com/1746-6148/8/35 |
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