Gut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinoma
Abstract Background Chronic hepatitis C is the most common cause of chronic liver disease and hepatocellular carcinoma in Egypt. A pathogenic link with gut microbial dysbiosis has been described in different diseases. The aim of the study is to elucidate changes in gut microbiome in cirrhotic HCV Eg...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-09-01
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| Series: | Egyptian Liver Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43066-021-00147-y |
| _version_ | 1818739878543753216 |
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| author | Khaled Mohieldeen Soraya Abdel Fatah Hamoda Shwikar Mahmoud Ahmed Abdurrahman Najeeb Walid Ismail Ellakany |
| author_facet | Khaled Mohieldeen Soraya Abdel Fatah Hamoda Shwikar Mahmoud Ahmed Abdurrahman Najeeb Walid Ismail Ellakany |
| author_sort | Khaled Mohieldeen |
| collection | DOAJ |
| description | Abstract Background Chronic hepatitis C is the most common cause of chronic liver disease and hepatocellular carcinoma in Egypt. A pathogenic link with gut microbial dysbiosis has been described in different diseases. The aim of the study is to elucidate changes in gut microbiome in cirrhotic HCV Egyptian patients with and without HCC. Results The study included 50 cirrhotic HCV patients; 25 with and 25 without HCC and 25 healthy controls with matched age and sex. Stool specimens were taken from all participants. Quantitative SYBR Green real-time PCR technique targeting 16S rRNA was done for the identification and quantitation of selected bacterial phylum, genera, and/or species. Both HCC and cirrhosis groups showed decrease in Firmicutes, F/B ratio, A. mucinophilia, and F. prausnitzii compared to the control group. However, the HCC group only showed statistically significant increase in Bacteroides and Lactobacilli, and decrease of Prevotella relative abundance and P/B ratio compared to both cirrhosis and control groups. As regard the relation between the gut microbiome and stages of HCC, BCLC stage D showed significantly the lowest relative abundance of Ruminococcus. Conclusion Patients with HCV-related cirrhosis and HCC exhibit microbial dysbiosis; altered microbial relative abundance and diversity with HCC patients showing higher proinflammatory bacteria compared to cirrhotics. |
| first_indexed | 2024-12-18T01:31:50Z |
| format | Article |
| id | doaj.art-9c83bef5157d4ffb82b00ae280d50eab |
| institution | Directory Open Access Journal |
| issn | 2090-6226 |
| language | English |
| last_indexed | 2024-12-18T01:31:50Z |
| publishDate | 2021-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Liver Journal |
| spelling | doaj.art-9c83bef5157d4ffb82b00ae280d50eab2022-12-21T21:25:35ZengSpringerOpenEgyptian Liver Journal2090-62262021-09-011111910.1186/s43066-021-00147-yGut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinomaKhaled Mohieldeen0Soraya Abdel Fatah Hamoda1Shwikar Mahmoud Ahmed2Abdurrahman Najeeb3Walid Ismail Ellakany4Tropical Medicine Department, Faculty of Medicine, Alexandria UniversityTropical Medicine Department, Faculty of Medicine, Alexandria UniversityMedical Microbiology and Immunology Department, Faculty of Medicine, Alexandria UniversityTropical Medicine Department, Faculty of Medicine, Alexandria UniversityTropical Medicine Department, Faculty of Medicine, Alexandria UniversityAbstract Background Chronic hepatitis C is the most common cause of chronic liver disease and hepatocellular carcinoma in Egypt. A pathogenic link with gut microbial dysbiosis has been described in different diseases. The aim of the study is to elucidate changes in gut microbiome in cirrhotic HCV Egyptian patients with and without HCC. Results The study included 50 cirrhotic HCV patients; 25 with and 25 without HCC and 25 healthy controls with matched age and sex. Stool specimens were taken from all participants. Quantitative SYBR Green real-time PCR technique targeting 16S rRNA was done for the identification and quantitation of selected bacterial phylum, genera, and/or species. Both HCC and cirrhosis groups showed decrease in Firmicutes, F/B ratio, A. mucinophilia, and F. prausnitzii compared to the control group. However, the HCC group only showed statistically significant increase in Bacteroides and Lactobacilli, and decrease of Prevotella relative abundance and P/B ratio compared to both cirrhosis and control groups. As regard the relation between the gut microbiome and stages of HCC, BCLC stage D showed significantly the lowest relative abundance of Ruminococcus. Conclusion Patients with HCV-related cirrhosis and HCC exhibit microbial dysbiosis; altered microbial relative abundance and diversity with HCC patients showing higher proinflammatory bacteria compared to cirrhotics.https://doi.org/10.1186/s43066-021-00147-yCirrhosisHepatitis C virusHepatocellular carcinomaGut dysbiosis |
| spellingShingle | Khaled Mohieldeen Soraya Abdel Fatah Hamoda Shwikar Mahmoud Ahmed Abdurrahman Najeeb Walid Ismail Ellakany Gut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinoma Egyptian Liver Journal Cirrhosis Hepatitis C virus Hepatocellular carcinoma Gut dysbiosis |
| title | Gut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinoma |
| title_full | Gut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinoma |
| title_fullStr | Gut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinoma |
| title_full_unstemmed | Gut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinoma |
| title_short | Gut microbiome in cirrhotic hepatitis C virus patients with and without hepatocellular carcinoma |
| title_sort | gut microbiome in cirrhotic hepatitis c virus patients with and without hepatocellular carcinoma |
| topic | Cirrhosis Hepatitis C virus Hepatocellular carcinoma Gut dysbiosis |
| url | https://doi.org/10.1186/s43066-021-00147-y |
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