COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL
Background: Parkinson disease is characterized with deposition of Lewy Bodies containing α–synuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-lik...
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| Format: | Article |
| Language: | English |
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University of Brawijaya
2019-01-01
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| Series: | Malang Neurology Journal |
| Subjects: | |
| Online Access: | https://mnj.ub.ac.id/index.php/mnj/article/view/291 |
| _version_ | 1819004667019919360 |
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| author | Ayu Trisnawati Anasrulloh Anasrulloh Sri Budhi Rianawati Husnul Khotimah Mulyohadi Ali Budi Susetya |
| author_facet | Ayu Trisnawati Anasrulloh Anasrulloh Sri Budhi Rianawati Husnul Khotimah Mulyohadi Ali Budi Susetya |
| author_sort | Ayu Trisnawati |
| collection | DOAJ |
| description | Background: Parkinson disease is characterized with deposition of Lewy Bodies containing α–synuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-like 2 (Nrf2). Centella asiatica (C.asiatica) contains antioxidant effect, inhibits the aggregation of α–synuclein and improves the locomotor on Parkinson-model animals so it needs to compare to the standard medication.
Objective: To compare the C. asiatica extract and Pramipexole to the zebrafish Parkinson model by determining the locomotor activity, α–synuclein expression, and Nrf2.
Methods: This study used six groups of zebrafish: negative control, rotenone rotenone [5 μg/L], pramipexole1, 2, 3 (rotenone + pramipexole [3,5] ng/mL, [7] ng/mL, [14] ng/mL), and C. asiatica (rotenone + C. asiatica [10] μg/mL). The observations of locomotor activity of day 0, 14, and 28 were continued to the α–synuclein immunohistochemical examination, and Nrf2 on the midbrain area.
Results: There are significant differences in locomotor activity on day 28 among the C. asiatica group with rotenone (p<0,05), while there are no significant differences among the C. asiatica group with pramipexole [7] ng/mL and [14] ng/mL (p>0,05). α–synuclein expression of the C. asiatica group is the lowest and significantly different from all groups (p<0,05), while Nr2 had no significant differences (p>0,05).
Conclusion: C. asiatica extract [10] μg/mL is equal to pramipexole [7] ng/mL and [14] ng/mL in improving locomotor activity, but C. asiatica extract holds excellence as it decreases α–synuclein expression better than pramipexole, while Nrf2 expression shows no differences. |
| first_indexed | 2024-12-20T23:40:32Z |
| format | Article |
| id | doaj.art-9c84885d509f4c5b80334d89f5e4012f |
| institution | Directory Open Access Journal |
| issn | 2407-6724 2442-5001 |
| language | English |
| last_indexed | 2024-12-20T23:40:32Z |
| publishDate | 2019-01-01 |
| publisher | University of Brawijaya |
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| series | Malang Neurology Journal |
| spelling | doaj.art-9c84885d509f4c5b80334d89f5e4012f2022-12-21T19:23:06ZengUniversity of BrawijayaMalang Neurology Journal2407-67242442-50012019-01-015151310.21776/ub.mnj.2019.005.01.279COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODELAyu Trisnawati0Anasrulloh Anasrulloh 1Sri Budhi Rianawati2Husnul Khotimah3Mulyohadi Ali4Budi Susetya5Department of Neurology Faculty of Medicine Brawijaya University, Malang, Indonesia.Department of Neurology Faculty of Medicine Brawijaya University, Malang, Indonesia.Department of Neurology Faculty of Medicine Brawijaya University, Malang, Indonesia.Department of Pharmacology, Faculty of Medicine Brawijaya University, Malang, Indonesia.Department of Pharmacology, Faculty of Medicine Brawijaya University, Malang, Indonesia.Malang State Health Polytechnic, Malang, Indonesia.Background: Parkinson disease is characterized with deposition of Lewy Bodies containing α–synuclein happened due to the effect of chronic neuroinflammation that causes the death of dopaminergic neurons through oxidative stress processes, so it involves the response of Nuclear factor erythroid 2-like 2 (Nrf2). Centella asiatica (C.asiatica) contains antioxidant effect, inhibits the aggregation of α–synuclein and improves the locomotor on Parkinson-model animals so it needs to compare to the standard medication. Objective: To compare the C. asiatica extract and Pramipexole to the zebrafish Parkinson model by determining the locomotor activity, α–synuclein expression, and Nrf2. Methods: This study used six groups of zebrafish: negative control, rotenone rotenone [5 μg/L], pramipexole1, 2, 3 (rotenone + pramipexole [3,5] ng/mL, [7] ng/mL, [14] ng/mL), and C. asiatica (rotenone + C. asiatica [10] μg/mL). The observations of locomotor activity of day 0, 14, and 28 were continued to the α–synuclein immunohistochemical examination, and Nrf2 on the midbrain area. Results: There are significant differences in locomotor activity on day 28 among the C. asiatica group with rotenone (p<0,05), while there are no significant differences among the C. asiatica group with pramipexole [7] ng/mL and [14] ng/mL (p>0,05). α–synuclein expression of the C. asiatica group is the lowest and significantly different from all groups (p<0,05), while Nr2 had no significant differences (p>0,05). Conclusion: C. asiatica extract [10] μg/mL is equal to pramipexole [7] ng/mL and [14] ng/mL in improving locomotor activity, but C. asiatica extract holds excellence as it decreases α–synuclein expression better than pramipexole, while Nrf2 expression shows no differences.https://mnj.ub.ac.id/index.php/mnj/article/view/291centella asiaticalocomotor activityα–synucleinnrf2parkinson diseasezebrafish |
| spellingShingle | Ayu Trisnawati Anasrulloh Anasrulloh Sri Budhi Rianawati Husnul Khotimah Mulyohadi Ali Budi Susetya COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL Malang Neurology Journal centella asiatica locomotor activity α–synuclein nrf2 parkinson disease zebrafish |
| title | COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL |
| title_full | COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL |
| title_fullStr | COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL |
| title_full_unstemmed | COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL |
| title_short | COMPARISON EFFECT BETWEEN PEGAGAN (Centella asiatica) EXTRACT AND PRAMIPEXOLE TOWARD LOCOMOTOR ACTIVITIES, α - SYNUCLEIN, AND NRF2 EXPRESSION IN ZEBRAFISH PARKINSON MODEL |
| title_sort | comparison effect between pegagan centella asiatica extract and pramipexole toward locomotor activities α synuclein and nrf2 expression in zebrafish parkinson model |
| topic | centella asiatica locomotor activity α–synuclein nrf2 parkinson disease zebrafish |
| url | https://mnj.ub.ac.id/index.php/mnj/article/view/291 |
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