Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant Activity
The present study aimed to investigate the ability of <i>Lactobacillus plantarum</i> TA4 in tolerating Ag<sup>+</sup> and its ability to produce silver nanoparticles (AgNPs). The biosynthesized AgNPs were characterized using UV–Visible spectroscopy (UV–Vis), dynamic light sca...
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MDPI AG
2020-10-01
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| author | Hidayat Mohd Yusof Nor’Aini Abdul Rahman Rosfarizan Mohamad Uswatun Hasanah Zaidan |
| author_facet | Hidayat Mohd Yusof Nor’Aini Abdul Rahman Rosfarizan Mohamad Uswatun Hasanah Zaidan |
| author_sort | Hidayat Mohd Yusof |
| collection | DOAJ |
| description | The present study aimed to investigate the ability of <i>Lactobacillus plantarum</i> TA4 in tolerating Ag<sup>+</sup> and its ability to produce silver nanoparticles (AgNPs). The biosynthesized AgNPs were characterized using UV–Visible spectroscopy (UV–Vis), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and high-resolution transmission electron microscope (HR-TEM). The cell biomass of <i>L. plantarum</i> TA4 demonstrated the ability to tolerate Ag<sup>+</sup> at a concentration of 2 mM, followed by the formation of AgNPs. This was confirmed by the visual observation of color changes and a presence of maximum UV–Vis absorption centered at 429 nm. HR-TEM analysis revealed that the AgNPs were spherical with an average size of 14.0 ± 4.7 nm, while the SEM-EDX analysis detected that the particles were primarily located on the cell membrane of <i>L. plantarum</i> TA4. Further, DLS analysis revealed that the polydispersity index (PDI) value of biosynthesized AgNPs was 0.193, implying the monodispersed characteristic of NPs. Meanwhile, the FTIR study confirmed the involvement of functional groups from the cell biomass that involved in the reduction process. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens in a concentration-dependent manner. Furthermore, the antioxidant property of biosynthesized AgNPs that was evaluated using the DPPH assay showed considerable antioxidant potential. Results from this study provide a sustainable and inexpensive method for the production of AgNPs. |
| first_indexed | 2024-03-10T15:49:58Z |
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| language | English |
| last_indexed | 2024-03-10T15:49:58Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj.art-9c8498cf673c4b40ac0ff530551458202023-11-20T16:09:21ZengMDPI AGApplied Sciences2076-34172020-10-011019697310.3390/app10196973Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant ActivityHidayat Mohd Yusof0Nor’Aini Abdul Rahman1Rosfarizan Mohamad2Uswatun Hasanah Zaidan3Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaDepartment of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, MalaysiaThe present study aimed to investigate the ability of <i>Lactobacillus plantarum</i> TA4 in tolerating Ag<sup>+</sup> and its ability to produce silver nanoparticles (AgNPs). The biosynthesized AgNPs were characterized using UV–Visible spectroscopy (UV–Vis), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and high-resolution transmission electron microscope (HR-TEM). The cell biomass of <i>L. plantarum</i> TA4 demonstrated the ability to tolerate Ag<sup>+</sup> at a concentration of 2 mM, followed by the formation of AgNPs. This was confirmed by the visual observation of color changes and a presence of maximum UV–Vis absorption centered at 429 nm. HR-TEM analysis revealed that the AgNPs were spherical with an average size of 14.0 ± 4.7 nm, while the SEM-EDX analysis detected that the particles were primarily located on the cell membrane of <i>L. plantarum</i> TA4. Further, DLS analysis revealed that the polydispersity index (PDI) value of biosynthesized AgNPs was 0.193, implying the monodispersed characteristic of NPs. Meanwhile, the FTIR study confirmed the involvement of functional groups from the cell biomass that involved in the reduction process. Moreover, biosynthesized AgNPs exhibited antibacterial activity against Gram-positive and Gram-negative pathogens in a concentration-dependent manner. Furthermore, the antioxidant property of biosynthesized AgNPs that was evaluated using the DPPH assay showed considerable antioxidant potential. Results from this study provide a sustainable and inexpensive method for the production of AgNPs.https://www.mdpi.com/2076-3417/10/19/6973antibacterialbiosynthesisFTIRHR-TEM<i>Lactobacillus</i>silver nanoparticles |
| spellingShingle | Hidayat Mohd Yusof Nor’Aini Abdul Rahman Rosfarizan Mohamad Uswatun Hasanah Zaidan Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant Activity Applied Sciences antibacterial biosynthesis FTIR HR-TEM <i>Lactobacillus</i> silver nanoparticles |
| title | Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant Activity |
| title_full | Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant Activity |
| title_fullStr | Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant Activity |
| title_full_unstemmed | Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant Activity |
| title_short | Microbial Mediated Synthesis of Silver Nanoparticles by <i>Lactobacillus Plantarum</i> TA4 and its Antibacterial and Antioxidant Activity |
| title_sort | microbial mediated synthesis of silver nanoparticles by i lactobacillus plantarum i ta4 and its antibacterial and antioxidant activity |
| topic | antibacterial biosynthesis FTIR HR-TEM <i>Lactobacillus</i> silver nanoparticles |
| url | https://www.mdpi.com/2076-3417/10/19/6973 |
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