Integrated analysis of the Plasmodium species transcriptome
The genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene ex...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-05-01
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| Series: | EBioMedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396416301475 |
| _version_ | 1811223585531887616 |
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| author | Regina Hoo Lei Zhu Anburaj Amaladoss Sachel Mok Onguma Natalang Stacey A. Lapp Guangan Hu Kingsley Liew Mary R. Galinski Zbynek Bozdech Peter R. Preiser |
| author_facet | Regina Hoo Lei Zhu Anburaj Amaladoss Sachel Mok Onguma Natalang Stacey A. Lapp Guangan Hu Kingsley Liew Mary R. Galinski Zbynek Bozdech Peter R. Preiser |
| author_sort | Regina Hoo |
| collection | DOAJ |
| description | The genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene expression play an important role in defining species-specific characteristics. Here, we developed a comprehensive approach to measure transcriptional changes of the evolutionary conserved syntenic orthologs during the intraerythrocytic developmental cycle across six Plasmodium species. We show significant transcriptional constraint at the mid-developmental stage of Plasmodium species while the earliest stages of parasite development display the greatest transcriptional variation associated with critical functional processes. Modeling of the evolutionary relationship based on changes in transcriptional profile reveal a phylogeny pattern of the Plasmodium species that strictly follows its mammalian hosts. In addition, the work shows that transcriptional conserved orthologs represent potential future targets for anti-malaria intervention as they would be expected to carry out key essential functions within the parasites. This work provides an integrated analysis of orthologous transcriptome, which aims to provide insights into the Plasmodium evolution thereby establishing a framework to explore complex pathways and drug discovery in Plasmodium species with broad host range. |
| first_indexed | 2024-04-12T08:35:17Z |
| format | Article |
| id | doaj.art-9c858bda6e8742c98a19aa3cf7a84a41 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-04-12T08:35:17Z |
| publishDate | 2016-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-9c858bda6e8742c98a19aa3cf7a84a412022-12-22T03:40:02ZengElsevierEBioMedicine2352-39642016-05-017C25526610.1016/j.ebiom.2016.04.011Integrated analysis of the Plasmodium species transcriptomeRegina Hoo0Lei Zhu1Anburaj Amaladoss2Sachel Mok3Onguma Natalang4Stacey A. Lapp5Guangan Hu6Kingsley Liew7Mary R. Galinski8Zbynek Bozdech9Peter R. Preiser10School of Biological Sciences, Nanyang Technological University, SingaporeSchool of Biological Sciences, Nanyang Technological University, SingaporeSchool of Biological Sciences, Nanyang Technological University, SingaporeSchool of Biological Sciences, Nanyang Technological University, SingaporeSchool of Biological Sciences, Nanyang Technological University, SingaporeEmory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USASchool of Biological Sciences, Nanyang Technological University, SingaporeSchool of Biological Sciences, Nanyang Technological University, SingaporeEmory Vaccine Center, Yerkes National Primate Research Center, Emory University, Atlanta, GA, USASchool of Biological Sciences, Nanyang Technological University, SingaporeSchool of Biological Sciences, Nanyang Technological University, SingaporeThe genome sequence available for different Plasmodium species is a valuable resource for understanding malaria parasite biology. However, comparative genomics on its own cannot fully explain all the species-specific differences which suggests that other genomic aspects such as regulation of gene expression play an important role in defining species-specific characteristics. Here, we developed a comprehensive approach to measure transcriptional changes of the evolutionary conserved syntenic orthologs during the intraerythrocytic developmental cycle across six Plasmodium species. We show significant transcriptional constraint at the mid-developmental stage of Plasmodium species while the earliest stages of parasite development display the greatest transcriptional variation associated with critical functional processes. Modeling of the evolutionary relationship based on changes in transcriptional profile reveal a phylogeny pattern of the Plasmodium species that strictly follows its mammalian hosts. In addition, the work shows that transcriptional conserved orthologs represent potential future targets for anti-malaria intervention as they would be expected to carry out key essential functions within the parasites. This work provides an integrated analysis of orthologous transcriptome, which aims to provide insights into the Plasmodium evolution thereby establishing a framework to explore complex pathways and drug discovery in Plasmodium species with broad host range.http://www.sciencedirect.com/science/article/pii/S2352396416301475Comparative transcriptomicsPlasmodium speciesEvolutionMicroarrayTranscriptomeDrug targets |
| spellingShingle | Regina Hoo Lei Zhu Anburaj Amaladoss Sachel Mok Onguma Natalang Stacey A. Lapp Guangan Hu Kingsley Liew Mary R. Galinski Zbynek Bozdech Peter R. Preiser Integrated analysis of the Plasmodium species transcriptome EBioMedicine Comparative transcriptomics Plasmodium species Evolution Microarray Transcriptome Drug targets |
| title | Integrated analysis of the Plasmodium species transcriptome |
| title_full | Integrated analysis of the Plasmodium species transcriptome |
| title_fullStr | Integrated analysis of the Plasmodium species transcriptome |
| title_full_unstemmed | Integrated analysis of the Plasmodium species transcriptome |
| title_short | Integrated analysis of the Plasmodium species transcriptome |
| title_sort | integrated analysis of the plasmodium species transcriptome |
| topic | Comparative transcriptomics Plasmodium species Evolution Microarray Transcriptome Drug targets |
| url | http://www.sciencedirect.com/science/article/pii/S2352396416301475 |
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