Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland
Fusarium head blight (FHB) of cereals is the major head disease negatively affecting grain production worldwide. In 2016 and 2017, serious outbreaks of FHB occurred in wheat crops in Poland. In this study, we characterized the diversity of Fusaria responsible for these epidemics using TaqMan assays....
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MDPI AG
2018-08-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/8/325 |
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| author | Katarzyna Bilska Sebastian Jurczak Tomasz Kulik Ewa Ropelewska Jacek Olszewski Maciej Żelechowski Piotr Zapotoczny |
| author_facet | Katarzyna Bilska Sebastian Jurczak Tomasz Kulik Ewa Ropelewska Jacek Olszewski Maciej Żelechowski Piotr Zapotoczny |
| author_sort | Katarzyna Bilska |
| collection | DOAJ |
| description | Fusarium head blight (FHB) of cereals is the major head disease negatively affecting grain production worldwide. In 2016 and 2017, serious outbreaks of FHB occurred in wheat crops in Poland. In this study, we characterized the diversity of Fusaria responsible for these epidemics using TaqMan assays. From a panel of 463 field isolates collected from wheat, four Fusarium species were identified. The predominant species were F. graminearum s.s. (81%) and, to a lesser extent, F. avenaceum (15%). The emergence of the 15ADON genotype was found ranging from 83% to 87% of the total trichothecene genotypes isolated in 2016 and 2017, respectively. Our results indicate two dramatic shifts within fungal field populations in Poland. The first shift is associated with the displacement of F. culmorum by F. graminearum s.s. The second shift resulted from a loss of nivalenol genotypes. We suggest that an emerging prevalence of F. graminearum s.s. may be linked to boosted maize production, which has increased substantially over the last decade in Poland. To detect variation within Tri core clusters, we compared sequence data from randomly selected field isolates with a panel of strains from geographically diverse origins. We found that the newly emerged 15ADON genotypes do not exhibit a specific pattern of polymorphism enabling their clear differentiation from the other European strains. |
| first_indexed | 2024-04-11T20:42:44Z |
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| id | doaj.art-9c8734faca9141fd81a848923bc80a22 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T20:42:44Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-9c8734faca9141fd81a848923bc80a222022-12-22T04:04:10ZengMDPI AGToxins2072-66512018-08-0110832510.3390/toxins10080325toxins10080325Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in PolandKatarzyna Bilska0Sebastian Jurczak1Tomasz Kulik2Ewa Ropelewska3Jacek Olszewski4Maciej Żelechowski5Piotr Zapotoczny6Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727 Olsztyn, PolandDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727 Olsztyn, PolandDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727 Olsztyn, PolandDepartment of Systems Engineering, Faculty of Engineering, University of Warmia and Mazury in Olsztyn, Heweliusza 14, 10-718 Olsztyn, PolandExperimental Education Unit, Oczapowskiego 8, 10-719 Olsztyn, PolandDepartment of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727 Olsztyn, PolandDepartment of Systems Engineering, Faculty of Engineering, University of Warmia and Mazury in Olsztyn, Heweliusza 14, 10-718 Olsztyn, PolandFusarium head blight (FHB) of cereals is the major head disease negatively affecting grain production worldwide. In 2016 and 2017, serious outbreaks of FHB occurred in wheat crops in Poland. In this study, we characterized the diversity of Fusaria responsible for these epidemics using TaqMan assays. From a panel of 463 field isolates collected from wheat, four Fusarium species were identified. The predominant species were F. graminearum s.s. (81%) and, to a lesser extent, F. avenaceum (15%). The emergence of the 15ADON genotype was found ranging from 83% to 87% of the total trichothecene genotypes isolated in 2016 and 2017, respectively. Our results indicate two dramatic shifts within fungal field populations in Poland. The first shift is associated with the displacement of F. culmorum by F. graminearum s.s. The second shift resulted from a loss of nivalenol genotypes. We suggest that an emerging prevalence of F. graminearum s.s. may be linked to boosted maize production, which has increased substantially over the last decade in Poland. To detect variation within Tri core clusters, we compared sequence data from randomly selected field isolates with a panel of strains from geographically diverse origins. We found that the newly emerged 15ADON genotypes do not exhibit a specific pattern of polymorphism enabling their clear differentiation from the other European strains.http://www.mdpi.com/2072-6651/10/8/325FusariumFusarium head blighttrichothecenesgenotypesTri core clusterqPCRwheat |
| spellingShingle | Katarzyna Bilska Sebastian Jurczak Tomasz Kulik Ewa Ropelewska Jacek Olszewski Maciej Żelechowski Piotr Zapotoczny Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland Toxins Fusarium Fusarium head blight trichothecenes genotypes Tri core cluster qPCR wheat |
| title | Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland |
| title_full | Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland |
| title_fullStr | Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland |
| title_full_unstemmed | Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland |
| title_short | Species Composition and Trichothecene Genotype Profiling of Fusarium Field Isolates Recovered from Wheat in Poland |
| title_sort | species composition and trichothecene genotype profiling of fusarium field isolates recovered from wheat in poland |
| topic | Fusarium Fusarium head blight trichothecenes genotypes Tri core cluster qPCR wheat |
| url | http://www.mdpi.com/2072-6651/10/8/325 |
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