Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cells
UDP-glucuronosyltransferases (UGTs) are important detoxification and drug-metabolizing enzymes, which catalyse the glucuronidation of exogenous and endogenous chemicals. The anti-carcinogenic activity of dietary phytochemicals is partly attributed to the induction of phase II enzymes, including UGT1...
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| Format: | Article |
| Language: | English |
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Poznan University of Medical Sciences
2014-09-01
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| Series: | Journal of Medical Science |
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| Online Access: | https://jms.ump.edu.pl/index.php/JMS/article/view/69 |
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| author | Violetta Krajka-Kuźniak Adam Krysztofiak Jarosław Paluszczak |
| author_facet | Violetta Krajka-Kuźniak Adam Krysztofiak Jarosław Paluszczak |
| author_sort | Violetta Krajka-Kuźniak |
| collection | DOAJ |
| description | UDP-glucuronosyltransferases (UGTs) are important detoxification and drug-metabolizing enzymes, which catalyse the glucuronidation of exogenous and endogenous chemicals. The anti-carcinogenic activity of dietary phytochemicals is partly attributed to the induction of phase II enzymes, including UGT1A. Our earlier study showed that protocatechuic acid increased UGT activity in rat liver. A similar effect was observed for indole-3-carbinol and phenethyl isothiocyanate in rat liver. In this study we assessed the effect of protocatechuic acid, tannic acid, indole-3-carbinol and phenethyl isothiocyanate on the expression and protein level of UGT1A in hepatocellular carcinoma HepG2 cells. Cells were incubated with 2µM and 10µM of protocatechuic acid, tannic acid, or indole-3-carbinol and 1µM and 5µM of phenethyl isothiocyanate for 72 hours. Transcript level was measured by RT-PCR and protein level by the immunoblot assay. Treatment with protocatechuic acid, tannic acid, indole-3-carbinol and phenethyl isothiocyanate induced the expression and protein level of UGT1A. Phenethyl isothiocyanate increased the mRNA of UGT1A to the most extent (0.28–5.7 fold change, p < 0.05). Increased expression of UGT1A was accompanied by the enhancement of its protein level, with the exception of protocatechuic acid at the dose of 2µM. Overall, isothiocyanates and indoles were more potent as UGT inducers than phenolic acids. Collectively, the results suggest that the induction of UGT1A could contribute to the hepatoprotective and chemopreventive properties of these phytochemicals. |
| first_indexed | 2024-12-22T10:21:56Z |
| format | Article |
| id | doaj.art-9c878b110e294a1c9c5a209c39988667 |
| institution | Directory Open Access Journal |
| issn | 2353-9798 2353-9801 |
| language | English |
| last_indexed | 2024-12-22T10:21:56Z |
| publishDate | 2014-09-01 |
| publisher | Poznan University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Science |
| spelling | doaj.art-9c878b110e294a1c9c5a209c399886672022-12-21T18:29:36ZengPoznan University of Medical SciencesJournal of Medical Science2353-97982353-98012014-09-0183310.20883/medical.e69Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cellsVioletta Krajka-Kuźniak0Adam Krysztofiak1Jarosław Paluszczak2Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, PolandDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, PolandDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, PolandUDP-glucuronosyltransferases (UGTs) are important detoxification and drug-metabolizing enzymes, which catalyse the glucuronidation of exogenous and endogenous chemicals. The anti-carcinogenic activity of dietary phytochemicals is partly attributed to the induction of phase II enzymes, including UGT1A. Our earlier study showed that protocatechuic acid increased UGT activity in rat liver. A similar effect was observed for indole-3-carbinol and phenethyl isothiocyanate in rat liver. In this study we assessed the effect of protocatechuic acid, tannic acid, indole-3-carbinol and phenethyl isothiocyanate on the expression and protein level of UGT1A in hepatocellular carcinoma HepG2 cells. Cells were incubated with 2µM and 10µM of protocatechuic acid, tannic acid, or indole-3-carbinol and 1µM and 5µM of phenethyl isothiocyanate for 72 hours. Transcript level was measured by RT-PCR and protein level by the immunoblot assay. Treatment with protocatechuic acid, tannic acid, indole-3-carbinol and phenethyl isothiocyanate induced the expression and protein level of UGT1A. Phenethyl isothiocyanate increased the mRNA of UGT1A to the most extent (0.28–5.7 fold change, p < 0.05). Increased expression of UGT1A was accompanied by the enhancement of its protein level, with the exception of protocatechuic acid at the dose of 2µM. Overall, isothiocyanates and indoles were more potent as UGT inducers than phenolic acids. Collectively, the results suggest that the induction of UGT1A could contribute to the hepatoprotective and chemopreventive properties of these phytochemicals.https://jms.ump.edu.pl/index.php/JMS/article/view/69tannic acidprotocatechuic acidindole-3-carbinolphenethyl isothiocyanateUGT1AHepG2 |
| spellingShingle | Violetta Krajka-Kuźniak Adam Krysztofiak Jarosław Paluszczak Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cells Journal of Medical Science tannic acid protocatechuic acid indole-3-carbinol phenethyl isothiocyanate UGT1A HepG2 |
| title | Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cells |
| title_full | Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cells |
| title_fullStr | Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cells |
| title_full_unstemmed | Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cells |
| title_short | Induction of UDP-glucuronosyltransferase 1A by naturally occurring phytochemicals in human hepatoma cells |
| title_sort | induction of udp glucuronosyltransferase 1a by naturally occurring phytochemicals in human hepatoma cells |
| topic | tannic acid protocatechuic acid indole-3-carbinol phenethyl isothiocyanate UGT1A HepG2 |
| url | https://jms.ump.edu.pl/index.php/JMS/article/view/69 |
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