Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma
Schisantherin A (STA) is a traditional Chinese medicine extracted from the plant Schisandra chinensis, which has a wide range of anti-inflammatory, antioxidant, and other pharmacological effects. This study investigates the anti-hepatocellular carcinoma effects of STA and the underlying mechanisms....
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.1019486/full |
| _version_ | 1797985053928587264 |
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| author | Fan Feng Lianhong Pan Jiaqin Wu Mingying Liu Long He Li Yang Wei Zhou |
| author_facet | Fan Feng Lianhong Pan Jiaqin Wu Mingying Liu Long He Li Yang Wei Zhou |
| author_sort | Fan Feng |
| collection | DOAJ |
| description | Schisantherin A (STA) is a traditional Chinese medicine extracted from the plant Schisandra chinensis, which has a wide range of anti-inflammatory, antioxidant, and other pharmacological effects. This study investigates the anti-hepatocellular carcinoma effects of STA and the underlying mechanisms. STA significantly inhibits the proliferation and migration of Hep3B and HCCLM3 cells in vitro in a concentration-dependent manner. RNA-sequencing showed that 77 genes are upregulated and 136 genes are downregulated in STA-treated cells compared with untreated cells. KEGG pathway analysis showed significant enrichment in galactose metabolism as well as in fructose and mannose metabolism. Further gas chromatography-mass spectrometric analysis (GC-MS) confirmed this, indicating that STA significantly inhibits the glucose metabolism pathway of Hep3B cells. Tumor xenograft in nude mice showed that STA has a significant inhibitory effect on tumor growth in vivo. In conclusion, our results indicate that STA can inhibit cell proliferation by regulating glucose metabolism, with subsequent anti-tumor effects, and has the potential to be a candidate drug for the treatment of liver cancer. |
| first_indexed | 2024-04-11T07:11:26Z |
| format | Article |
| id | doaj.art-9c87ad5e91a84b2fb63e7c6db0a2001e |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-11T07:11:26Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-9c87ad5e91a84b2fb63e7c6db0a2001e2022-12-22T04:38:11ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-11-011310.3389/fphar.2022.10194861019486Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinomaFan Feng0Lianhong Pan1Jiaqin Wu2Mingying Liu3Long He4Li Yang5Wei Zhou6National Innovation and Attracting Talents “111” Base, Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Ministry of Education, Chongqing University, Chongqing, ChinaChongqing Engineering Research Center of Antitumor Natural Drugs, Chongqing Three Gorges Medical College, Chongqing, ChinaNational Innovation and Attracting Talents “111” Base, Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Ministry of Education, Chongqing University, Chongqing, ChinaSchool of Comprehensive Health Management, XiHua University, Chengdu, Sichuan, ChinaSchool of Artificial Intelligence, Chongqing University of Education, Chongqing, ChinaNational Innovation and Attracting Talents “111” Base, Key Laboratory of Biorheological Science and Technology, College of Bioengineering, Ministry of Education, Chongqing University, Chongqing, ChinaChongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, ChinaSchisantherin A (STA) is a traditional Chinese medicine extracted from the plant Schisandra chinensis, which has a wide range of anti-inflammatory, antioxidant, and other pharmacological effects. This study investigates the anti-hepatocellular carcinoma effects of STA and the underlying mechanisms. STA significantly inhibits the proliferation and migration of Hep3B and HCCLM3 cells in vitro in a concentration-dependent manner. RNA-sequencing showed that 77 genes are upregulated and 136 genes are downregulated in STA-treated cells compared with untreated cells. KEGG pathway analysis showed significant enrichment in galactose metabolism as well as in fructose and mannose metabolism. Further gas chromatography-mass spectrometric analysis (GC-MS) confirmed this, indicating that STA significantly inhibits the glucose metabolism pathway of Hep3B cells. Tumor xenograft in nude mice showed that STA has a significant inhibitory effect on tumor growth in vivo. In conclusion, our results indicate that STA can inhibit cell proliferation by regulating glucose metabolism, with subsequent anti-tumor effects, and has the potential to be a candidate drug for the treatment of liver cancer.https://www.frontiersin.org/articles/10.3389/fphar.2022.1019486/fullschisantherin Ahepatocellular carcinomafructose and pentose phosphate metabolismgalactose metabolismtraditional Chinese medicine |
| spellingShingle | Fan Feng Lianhong Pan Jiaqin Wu Mingying Liu Long He Li Yang Wei Zhou Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma Frontiers in Pharmacology schisantherin A hepatocellular carcinoma fructose and pentose phosphate metabolism galactose metabolism traditional Chinese medicine |
| title | Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma |
| title_full | Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma |
| title_fullStr | Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma |
| title_full_unstemmed | Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma |
| title_short | Schisantherin A inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma |
| title_sort | schisantherin a inhibits cell proliferation by regulating glucose metabolism pathway in hepatocellular carcinoma |
| topic | schisantherin A hepatocellular carcinoma fructose and pentose phosphate metabolism galactose metabolism traditional Chinese medicine |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.1019486/full |
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