A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish
Zebrafish (Danio rerio) has been used as a promising animal model to study gonadal development and gametogenesis. Although previous studies have identified critical molecules participating in zebrafish gonad differentiation, a landscape view of the biological processes involved in this process is st...
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KeAi Communications Co. Ltd.
2022-08-01
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| Series: | Water Biology and Security |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772735122000798 |
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| author | Ding Ye Yi-Xuan Tu Houpeng Wang Mudan He Yaqing Wang Zhengfang Chen Zhen-Xia Chen Yonghua Sun |
| author_facet | Ding Ye Yi-Xuan Tu Houpeng Wang Mudan He Yaqing Wang Zhengfang Chen Zhen-Xia Chen Yonghua Sun |
| author_sort | Ding Ye |
| collection | DOAJ |
| description | Zebrafish (Danio rerio) has been used as a promising animal model to study gonadal development and gametogenesis. Although previous studies have identified critical molecules participating in zebrafish gonad differentiation, a landscape view of the biological processes involved in this process is still lacking. Here we isolated intact zebrafish differentiating gonads, at 25 days post-fertilization (dpf) and 30 dpf and conducted RNA-seq analyses on the juvenile gonads that tended to develop into ovaries or testes. Our study demonstrates that the juvenile ovary and testis at 25 dpf and 30 dpf are different at the biological process level. During ovary differentiation, the biological processes related to metabolic activities in the production of energy and maternal substances, RNA degradation, and DNA repair were enriched. During testis differentiation, the biological processes related to cell proliferation, differentiation, and morphogenesis were enriched, with a total of 15 signaling pathways. Notably, we reveal that the immune-related processes are extensively involved in the regulation of testis development. Overall, this study provides a landscape of differentiated biological processes and novel insights into the initiation of sex differentiation in zebrafish. |
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| institution | Directory Open Access Journal |
| issn | 2772-7351 |
| language | English |
| last_indexed | 2024-04-09T15:30:20Z |
| publishDate | 2022-08-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Water Biology and Security |
| spelling | doaj.art-9c8b985e0fd94d79958b79a14c1746052023-04-28T08:57:29ZengKeAi Communications Co. Ltd.Water Biology and Security2772-73512022-08-0113100059A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafishDing Ye0Yi-Xuan Tu1Houpeng Wang2Mudan He3Yaqing Wang4Zhengfang Chen5Zhen-Xia Chen6Yonghua Sun7State Key Laboratory of Freshwater Ecology and Biotechnology, Hongshan Laboratory, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Corresponding author. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Hubei Hongshan Laboratory, Wuhan, Hubei, 430072, China.Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, 518000, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518000, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hongshan Laboratory, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hongshan Laboratory, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, 430072, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hongshan Laboratory, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hongshan Laboratory, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hongshan Laboratory, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, 430072, China; Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China; Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen, 518000, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518000, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Hongshan Laboratory, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Corresponding author. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Hubei Hongshan Laboratory, Wuhan, Hubei, 430072, China.Zebrafish (Danio rerio) has been used as a promising animal model to study gonadal development and gametogenesis. Although previous studies have identified critical molecules participating in zebrafish gonad differentiation, a landscape view of the biological processes involved in this process is still lacking. Here we isolated intact zebrafish differentiating gonads, at 25 days post-fertilization (dpf) and 30 dpf and conducted RNA-seq analyses on the juvenile gonads that tended to develop into ovaries or testes. Our study demonstrates that the juvenile ovary and testis at 25 dpf and 30 dpf are different at the biological process level. During ovary differentiation, the biological processes related to metabolic activities in the production of energy and maternal substances, RNA degradation, and DNA repair were enriched. During testis differentiation, the biological processes related to cell proliferation, differentiation, and morphogenesis were enriched, with a total of 15 signaling pathways. Notably, we reveal that the immune-related processes are extensively involved in the regulation of testis development. Overall, this study provides a landscape of differentiated biological processes and novel insights into the initiation of sex differentiation in zebrafish.http://www.sciencedirect.com/science/article/pii/S2772735122000798Gonad differentiationMaternal materialsOvarySex differentiationSpermatogenesistestisZebrafish |
| spellingShingle | Ding Ye Yi-Xuan Tu Houpeng Wang Mudan He Yaqing Wang Zhengfang Chen Zhen-Xia Chen Yonghua Sun A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish Water Biology and Security Gonad differentiation Maternal materials Ovary Sex differentiation Spermatogenesistestis Zebrafish |
| title | A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish |
| title_full | A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish |
| title_fullStr | A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish |
| title_full_unstemmed | A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish |
| title_short | A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish |
| title_sort | landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish |
| topic | Gonad differentiation Maternal materials Ovary Sex differentiation Spermatogenesistestis Zebrafish |
| url | http://www.sciencedirect.com/science/article/pii/S2772735122000798 |
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