Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population
Abstract HPV16 and 18 are positively correlated with cervical carcinogenesis. However, HPV prevalence tends to vary according to region, nationality, and environment. The most prevalent high-risk (HR) HPV genotypes are HPV16, 52, 58, 56, 18, 33, and 45), while the low-risk (LR) genotypes are HPV6 an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-023-11529-3 |
| _version_ | 1797452323403857920 |
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| author | Nan Lv Yue Zhao Yiying Song Mingyu Ji Yunying Zhou |
| author_facet | Nan Lv Yue Zhao Yiying Song Mingyu Ji Yunying Zhou |
| author_sort | Nan Lv |
| collection | DOAJ |
| description | Abstract HPV16 and 18 are positively correlated with cervical carcinogenesis. However, HPV prevalence tends to vary according to region, nationality, and environment. The most prevalent high-risk (HR) HPV genotypes are HPV16, 52, 58, 56, 18, 33, and 45), while the low-risk (LR) genotypes are HPV6 and 11 in the Chinese population. Importantly, undetectable low-copy HPV DNA could be an important indicator of integration into the human genome and may be a precursor to cancer progression. The HPV viral load changes dramatically, either increasing or decreasing rapidly during carcinogenesis, and traditional quantitative real-time PCR (qPCR) cannot accurately capture this subtle change. Therefore, in this study, a reliable droplet digital PCR (ddPCR) method was developed to simultaneously detect and quantify HPV genotypes. The ddPCR quantitative results showed high accuracy, sensitivity, and specificity compared to qPCR results employing the same clinical specimens and supplemented the ddPCR assay for HPV52/56/58/6 genotypes according to the infection specificity of the Chinese population. In summary, this procedure is valuable for quantifying HPV DNA, especially under conditions of low template copy number in cervical intraepithelial neoplasia (CIN) and/or cervical cancer. Additionally, this method can dynamically observe the prognosis and outcome of HPV infection and thus be used as an effective means for real-time monitoring of tumor load. |
| first_indexed | 2024-03-09T15:06:53Z |
| format | Article |
| id | doaj.art-9c8f976d46df4c759def9b8fb717ff1c |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-03-09T15:06:53Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-9c8f976d46df4c759def9b8fb717ff1c2023-11-26T13:35:55ZengBMCBMC Cancer1471-24072023-10-012311910.1186/s12885-023-11529-3Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese populationNan Lv0Yue Zhao1Yiying Song2Mingyu Ji3Yunying Zhou4 Department of Clinical Laboratory Diagnosis, Jinan Central Hospital, Shandong University Department of Clinical Laboratory Diagnosis, Jinan Central Hospital, Shandong University Department of Clinical Laboratory Diagnosis, Jinan Central Hospital, Shandong UniversityMedical Research & Laboratory Diagnostic Center, Central Hospital Affiliated to Shandong First Medical University Department of Clinical Laboratory Diagnosis, Jinan Central Hospital, Shandong UniversityAbstract HPV16 and 18 are positively correlated with cervical carcinogenesis. However, HPV prevalence tends to vary according to region, nationality, and environment. The most prevalent high-risk (HR) HPV genotypes are HPV16, 52, 58, 56, 18, 33, and 45), while the low-risk (LR) genotypes are HPV6 and 11 in the Chinese population. Importantly, undetectable low-copy HPV DNA could be an important indicator of integration into the human genome and may be a precursor to cancer progression. The HPV viral load changes dramatically, either increasing or decreasing rapidly during carcinogenesis, and traditional quantitative real-time PCR (qPCR) cannot accurately capture this subtle change. Therefore, in this study, a reliable droplet digital PCR (ddPCR) method was developed to simultaneously detect and quantify HPV genotypes. The ddPCR quantitative results showed high accuracy, sensitivity, and specificity compared to qPCR results employing the same clinical specimens and supplemented the ddPCR assay for HPV52/56/58/6 genotypes according to the infection specificity of the Chinese population. In summary, this procedure is valuable for quantifying HPV DNA, especially under conditions of low template copy number in cervical intraepithelial neoplasia (CIN) and/or cervical cancer. Additionally, this method can dynamically observe the prognosis and outcome of HPV infection and thus be used as an effective means for real-time monitoring of tumor load.https://doi.org/10.1186/s12885-023-11529-3Human papillomavirusInfection specificityDroplet digital PCRViral load |
| spellingShingle | Nan Lv Yue Zhao Yiying Song Mingyu Ji Yunying Zhou Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population BMC Cancer Human papillomavirus Infection specificity Droplet digital PCR Viral load |
| title | Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population |
| title_full | Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population |
| title_fullStr | Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population |
| title_full_unstemmed | Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population |
| title_short | Development of a sensitive droplet digital PCR according to the HPV infection specificity in Chinese population |
| title_sort | development of a sensitive droplet digital pcr according to the hpv infection specificity in chinese population |
| topic | Human papillomavirus Infection specificity Droplet digital PCR Viral load |
| url | https://doi.org/10.1186/s12885-023-11529-3 |
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