Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways
Abstract Ethanol is a widely available carbon compound that can be increasingly produced with a net negative carbon balance. Carbon-negative ethanol might therefore provide a feedstock for building a wider range of sustainable chemicals. Here we show how ethanol can be converted with a cell free sys...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11653-3 |
| _version_ | 1817988357240651776 |
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| author | Hongjiang Liu Mark A. Arbing James U. Bowie |
| author_facet | Hongjiang Liu Mark A. Arbing James U. Bowie |
| author_sort | Hongjiang Liu |
| collection | DOAJ |
| description | Abstract Ethanol is a widely available carbon compound that can be increasingly produced with a net negative carbon balance. Carbon-negative ethanol might therefore provide a feedstock for building a wider range of sustainable chemicals. Here we show how ethanol can be converted with a cell free system into acetyl-CoA, a central precursor for myriad biochemicals, and how we can use the energy stored in ethanol to generate ATP, another key molecule important for powering biochemical pathways. The ATP generator produces acetone as a value-added side product. Our ATP generator reached titers of 27 ± 6 mM ATP and 59 ± 15 mM acetone with maximum ATP synthesis rate of 2.8 ± 0.6 mM/h and acetone of 7.8 ± 0.8 mM/h. We illustrated how the ATP generating module can power cell-free biochemical pathways by converting mevalonate into isoprenol at a titer of 12.5 ± 0.8 mM and a maximum productivity of 1.0 ± 0.05 mM/h. These proof-of-principle demonstrations may ultimately find their way to the manufacture of diverse chemicals from ethanol and other simple carbon compounds. |
| first_indexed | 2024-04-14T00:33:16Z |
| format | Article |
| id | doaj.art-9c8fbdbf61ac4fd3bc651470fe03f98b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-14T00:33:16Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9c8fbdbf61ac4fd3bc651470fe03f98b2022-12-22T02:22:29ZengNature PortfolioScientific Reports2045-23222022-05-0112111010.1038/s41598-022-11653-3Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathwaysHongjiang Liu0Mark A. Arbing1James U. Bowie2Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute of Genomics and Proteomics, University of California Los AngelesDepartment of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute of Genomics and Proteomics, University of California Los AngelesDepartment of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute of Genomics and Proteomics, University of California Los AngelesAbstract Ethanol is a widely available carbon compound that can be increasingly produced with a net negative carbon balance. Carbon-negative ethanol might therefore provide a feedstock for building a wider range of sustainable chemicals. Here we show how ethanol can be converted with a cell free system into acetyl-CoA, a central precursor for myriad biochemicals, and how we can use the energy stored in ethanol to generate ATP, another key molecule important for powering biochemical pathways. The ATP generator produces acetone as a value-added side product. Our ATP generator reached titers of 27 ± 6 mM ATP and 59 ± 15 mM acetone with maximum ATP synthesis rate of 2.8 ± 0.6 mM/h and acetone of 7.8 ± 0.8 mM/h. We illustrated how the ATP generating module can power cell-free biochemical pathways by converting mevalonate into isoprenol at a titer of 12.5 ± 0.8 mM and a maximum productivity of 1.0 ± 0.05 mM/h. These proof-of-principle demonstrations may ultimately find their way to the manufacture of diverse chemicals from ethanol and other simple carbon compounds.https://doi.org/10.1038/s41598-022-11653-3 |
| spellingShingle | Hongjiang Liu Mark A. Arbing James U. Bowie Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways Scientific Reports |
| title | Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways |
| title_full | Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways |
| title_fullStr | Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways |
| title_full_unstemmed | Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways |
| title_short | Expanding the use of ethanol as a feedstock for cell-free synthetic biochemistry by implementing acetyl-CoA and ATP generating pathways |
| title_sort | expanding the use of ethanol as a feedstock for cell free synthetic biochemistry by implementing acetyl coa and atp generating pathways |
| url | https://doi.org/10.1038/s41598-022-11653-3 |
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