Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farms
Abstract Background The estimation of the prevalence and zoonotic potential of Cryptosporidium parvum cycling in bovine populations requires the use of genotyping, as several morphologically similar non-parvum genetic variants of unproven clinical and public health impact are found in cattle. Howeve...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2015-04-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-015-0855-9 |
| _version_ | 1827933785647218688 |
|---|---|
| author | Julanda Al Mawly Alex Grinberg Niluka Velathanthiri Nigel French |
| author_facet | Julanda Al Mawly Alex Grinberg Niluka Velathanthiri Nigel French |
| author_sort | Julanda Al Mawly |
| collection | DOAJ |
| description | Abstract Background The estimation of the prevalence and zoonotic potential of Cryptosporidium parvum cycling in bovine populations requires the use of genotyping, as several morphologically similar non-parvum genetic variants of unproven clinical and public health impact are found in cattle. However, robust C. parvum prevalence estimates in cattle are lacking and comparative data of bovine and human isolates collected from the same regions are scarce. Thus, the relative contribution of the C. parvum oocysts released by farmed animals to animal and human cryptosporidiosis burden is, in general, poorly understood. Methods The New Zealand farm-level C. parvum prevalence was estimated using a cross-sectional sample of 1283 faecal specimens collected from newborn calves on 97 dairy farms. Faeces were analysed by immunofluorescence and the Cryptosporidium parasites were genetically identified. Finally, bovine C. parvum were genetically compared with historical human clinical isolates using a bilocus subtyping scheme. Results Immunofluoresence-positive faeces were found in 63/97 (65%) farms. C. parvum was identified in 49 (50.5%) farms, C. bovis in 6 (6.1%) farms, and on 8 (8.2%) farms the species could not be identified. The dominant C. parvum genetic variants were geographically widespread and found in both host populations, but several variants were found in humans only. Conclusions Phenotypic tests offered by New Zealand veterinary diagnostic laboratories for the diagnosis of C. parvum may have moderate to high positive predictive values for this species. The genetic similarities observed between the human and bovine parasites support a model considering calves as significant amplifiers of zoonotic C. parvum in New Zealand. However, data suggest that transmission routes not associated with dairy cattle should also be taken into account in future source-attribution studies of human cryptosporidiosis. |
| first_indexed | 2024-03-13T07:29:38Z |
| format | Article |
| id | doaj.art-9c937cdff38f47bc9c28d2487bd31f51 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:29:38Z |
| publishDate | 2015-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-9c937cdff38f47bc9c28d2487bd31f512023-06-04T11:10:54ZengBMCParasites & Vectors1756-33052015-04-01811710.1186/s13071-015-0855-9Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farmsJulanda Al Mawly0Alex Grinberg1Niluka Velathanthiri2Nigel French3mEpiLab, Hopkirk Research Institute, Massey UniversityInfectious Diseases Group, Institute of Veterinary, Animal and Biomedical Sciences, Massey UniversityInfectious Diseases Group, Institute of Veterinary, Animal and Biomedical Sciences, Massey UniversitymEpiLab, Hopkirk Research Institute, Massey UniversityAbstract Background The estimation of the prevalence and zoonotic potential of Cryptosporidium parvum cycling in bovine populations requires the use of genotyping, as several morphologically similar non-parvum genetic variants of unproven clinical and public health impact are found in cattle. However, robust C. parvum prevalence estimates in cattle are lacking and comparative data of bovine and human isolates collected from the same regions are scarce. Thus, the relative contribution of the C. parvum oocysts released by farmed animals to animal and human cryptosporidiosis burden is, in general, poorly understood. Methods The New Zealand farm-level C. parvum prevalence was estimated using a cross-sectional sample of 1283 faecal specimens collected from newborn calves on 97 dairy farms. Faeces were analysed by immunofluorescence and the Cryptosporidium parasites were genetically identified. Finally, bovine C. parvum were genetically compared with historical human clinical isolates using a bilocus subtyping scheme. Results Immunofluoresence-positive faeces were found in 63/97 (65%) farms. C. parvum was identified in 49 (50.5%) farms, C. bovis in 6 (6.1%) farms, and on 8 (8.2%) farms the species could not be identified. The dominant C. parvum genetic variants were geographically widespread and found in both host populations, but several variants were found in humans only. Conclusions Phenotypic tests offered by New Zealand veterinary diagnostic laboratories for the diagnosis of C. parvum may have moderate to high positive predictive values for this species. The genetic similarities observed between the human and bovine parasites support a model considering calves as significant amplifiers of zoonotic C. parvum in New Zealand. However, data suggest that transmission routes not associated with dairy cattle should also be taken into account in future source-attribution studies of human cryptosporidiosis.https://doi.org/10.1186/s13071-015-0855-9CryptosporidiumCalvesZoonosisDiarrheaPrevalence |
| spellingShingle | Julanda Al Mawly Alex Grinberg Niluka Velathanthiri Nigel French Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farms Parasites & Vectors Cryptosporidium Calves Zoonosis Diarrhea Prevalence |
| title | Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farms |
| title_full | Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farms |
| title_fullStr | Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farms |
| title_full_unstemmed | Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farms |
| title_short | Cross sectional study of prevalence, genetic diversity and zoonotic potential of Cryptosporidium parvum cycling in New Zealand dairy farms |
| title_sort | cross sectional study of prevalence genetic diversity and zoonotic potential of cryptosporidium parvum cycling in new zealand dairy farms |
| topic | Cryptosporidium Calves Zoonosis Diarrhea Prevalence |
| url | https://doi.org/10.1186/s13071-015-0855-9 |
| work_keys_str_mv | AT julandaalmawly crosssectionalstudyofprevalencegeneticdiversityandzoonoticpotentialofcryptosporidiumparvumcyclinginnewzealanddairyfarms AT alexgrinberg crosssectionalstudyofprevalencegeneticdiversityandzoonoticpotentialofcryptosporidiumparvumcyclinginnewzealanddairyfarms AT nilukavelathanthiri crosssectionalstudyofprevalencegeneticdiversityandzoonoticpotentialofcryptosporidiumparvumcyclinginnewzealanddairyfarms AT nigelfrench crosssectionalstudyofprevalencegeneticdiversityandzoonoticpotentialofcryptosporidiumparvumcyclinginnewzealanddairyfarms |