A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i>
The soil-borne oomycete pathogen <i>Aphanomyces euteiches</i> causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of <i>A. euteiches</i> have been shown to exhibit differential quantitative virulence, but the molecular basis of ho...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/8/1/88 |
| _version_ | 1797492841504571392 |
|---|---|
| author | Andrei Kiselev Hélène San Clemente Laurent Camborde Bernard Dumas Elodie Gaulin |
| author_facet | Andrei Kiselev Hélène San Clemente Laurent Camborde Bernard Dumas Elodie Gaulin |
| author_sort | Andrei Kiselev |
| collection | DOAJ |
| description | The soil-borne oomycete pathogen <i>Aphanomyces euteiches</i> causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of <i>A. euteiches</i> have been shown to exhibit differential quantitative virulence, but the molecular basis of host adaptation has not yet been clarified. Here, we re-sequenced a pea field reference strain of <i>A. euteiches</i> ATCC201684 with PacBio long-reads and took advantage of the technology to generate the mitochondrial genome. We identified that the secretome of <i>A. euteiches</i> is characterized by a large portfolio of secreted proteases and carbohydrate-active enzymes (CAZymes). We performed Illumina sequencing of four strains of <i>A. euteiches</i> with contrasted specificity to pea or alfalfa and found in different geographical areas. Comparative analysis showed that the core secretome is largely represented by CAZymes and proteases. The specific secretome is mainly composed of a large set of small, secreted proteins (SSP) without any predicted functional domain, suggesting that the legume preference of the pathogen is probably associated with unknown functions. This study forms the basis for further investigations into the mechanisms of interaction of <i>A. euteiches</i> with legumes. |
| first_indexed | 2024-03-10T01:10:29Z |
| format | Article |
| id | doaj.art-9c938e9f8ef6413a98d9e077883cc0e4 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T01:10:29Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-9c938e9f8ef6413a98d9e077883cc0e42023-11-23T14:18:50ZengMDPI AGJournal of Fungi2309-608X2022-01-01818810.3390/jof8010088A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i>Andrei Kiselev0Hélène San Clemente1Laurent Camborde2Bernard Dumas3Elodie Gaulin4Laboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, 31320 Toulouse, FranceLaboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, 31320 Toulouse, FranceLaboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, 31320 Toulouse, FranceLaboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, 31320 Toulouse, FranceLaboratoire de Recherche en Sciences Végétales (LRSV), Université de Toulouse, CNRS, UPS, Toulouse INP, 31320 Toulouse, FranceThe soil-borne oomycete pathogen <i>Aphanomyces euteiches</i> causes devastating root rot diseases in legumes such as pea and alfalfa. The different pathotypes of <i>A. euteiches</i> have been shown to exhibit differential quantitative virulence, but the molecular basis of host adaptation has not yet been clarified. Here, we re-sequenced a pea field reference strain of <i>A. euteiches</i> ATCC201684 with PacBio long-reads and took advantage of the technology to generate the mitochondrial genome. We identified that the secretome of <i>A. euteiches</i> is characterized by a large portfolio of secreted proteases and carbohydrate-active enzymes (CAZymes). We performed Illumina sequencing of four strains of <i>A. euteiches</i> with contrasted specificity to pea or alfalfa and found in different geographical areas. Comparative analysis showed that the core secretome is largely represented by CAZymes and proteases. The specific secretome is mainly composed of a large set of small, secreted proteins (SSP) without any predicted functional domain, suggesting that the legume preference of the pathogen is probably associated with unknown functions. This study forms the basis for further investigations into the mechanisms of interaction of <i>A. euteiches</i> with legumes.https://www.mdpi.com/2309-608X/8/1/88oomycete<i>Aphanomyces</i>secretomeeffectorlegumeadaptation |
| spellingShingle | Andrei Kiselev Hélène San Clemente Laurent Camborde Bernard Dumas Elodie Gaulin A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i> Journal of Fungi oomycete <i>Aphanomyces</i> secretome effector legume adaptation |
| title | A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i> |
| title_full | A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i> |
| title_fullStr | A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i> |
| title_full_unstemmed | A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i> |
| title_short | A Comprehensive Assessment of the Secretome Responsible for Host Adaptation of the Legume Root Pathogen <i>Aphanomyces euteiches</i> |
| title_sort | comprehensive assessment of the secretome responsible for host adaptation of the legume root pathogen i aphanomyces euteiches i |
| topic | oomycete <i>Aphanomyces</i> secretome effector legume adaptation |
| url | https://www.mdpi.com/2309-608X/8/1/88 |
| work_keys_str_mv | AT andreikiselev acomprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT helenesanclemente acomprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT laurentcamborde acomprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT bernarddumas acomprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT elodiegaulin acomprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT andreikiselev comprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT helenesanclemente comprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT laurentcamborde comprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT bernarddumas comprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi AT elodiegaulin comprehensiveassessmentofthesecretomeresponsibleforhostadaptationofthelegumerootpathogeniaphanomyceseuteichesi |