High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study
<i>Background and Objectives:</i> Oral squamous cell carcinoma (OSCC) accounts for about 95% of oral cancers. It represents a serious public health problem due to the high degree of morbidity and mortality, as well as multifactorial etiology. Human papillomavirus (HPV) infection is a wel...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/59/10/1843 |
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| author | Anđelija Petrović Miloš Čanković Miloš Avramov Željko D. Popović Srđa Janković Slavko Mojsilović |
| author_facet | Anđelija Petrović Miloš Čanković Miloš Avramov Željko D. Popović Srđa Janković Slavko Mojsilović |
| author_sort | Anđelija Petrović |
| collection | DOAJ |
| description | <i>Background and Objectives:</i> Oral squamous cell carcinoma (OSCC) accounts for about 95% of oral cancers. It represents a serious public health problem due to the high degree of morbidity and mortality, as well as multifactorial etiology. Human papillomavirus (HPV) infection is a well-documented risk factor for oropharyngeal carcinoma, but its role in oral carcinogenesis is still debatable. Our aim was to investigate the differences in the prevalence of high-risk HPV genotypes (HR-HPV) in patients with OSCC and oral potentially malignant disorders (OPMD) from that of healthy subjects. <i>Materials and Methods:</i> A total of 90 subjects were included in the cross-sectional study and divided into three groups of 30 patients each: (1) patients with OSCC, (2) patients with OPMD, and (3) healthy subjects. We examined the presence of 12 HR-HPV genotypes in the obtained biological material (oral swabs) using real-time PCR. <i>Results:</i> One or more of the 12 tested HR-HPV genotypes were detected in 5/30 patients with OSCC and 2/30 with OPMD, whereas no healthy subjects were positive for any of the tested genotypes. There was a statistically significant difference in nodal involvement between HPV-positive and HPV-negative patients with OSCC. <i>Conclusions:</i> Oral HR-HPV was detected in patients with oral premalignant and malignant lesions but not in healthy individuals, suggesting a possible role in oral carcinogenesis. Broad HR-HPV panel testing could increase the sensitivity of risk assessment and screening for OSCC. |
| first_indexed | 2024-03-10T21:03:48Z |
| format | Article |
| id | doaj.art-9c98cccfd08b4cc08aa0a08525893ae0 |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-10T21:03:48Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-9c98cccfd08b4cc08aa0a08525893ae02023-11-19T17:18:05ZengMDPI AGMedicina1010-660X1648-91442023-10-015910184310.3390/medicina59101843High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot StudyAnđelija Petrović0Miloš Čanković1Miloš Avramov2Željko D. Popović3Srđa Janković4Slavko Mojsilović5Group for Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, SerbiaDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, SerbiaDepartment of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 2, 21000 Novi Sad, SerbiaDivision of Immunology, Department of Hematology and Oncology, University Children’s Hospital, Tiršova 10, 11000 Belgrade, SerbiaGroup for Hematology and Stem Cells, Institute for Medical Research, University of Belgrade, 11000 Belgrade, Serbia<i>Background and Objectives:</i> Oral squamous cell carcinoma (OSCC) accounts for about 95% of oral cancers. It represents a serious public health problem due to the high degree of morbidity and mortality, as well as multifactorial etiology. Human papillomavirus (HPV) infection is a well-documented risk factor for oropharyngeal carcinoma, but its role in oral carcinogenesis is still debatable. Our aim was to investigate the differences in the prevalence of high-risk HPV genotypes (HR-HPV) in patients with OSCC and oral potentially malignant disorders (OPMD) from that of healthy subjects. <i>Materials and Methods:</i> A total of 90 subjects were included in the cross-sectional study and divided into three groups of 30 patients each: (1) patients with OSCC, (2) patients with OPMD, and (3) healthy subjects. We examined the presence of 12 HR-HPV genotypes in the obtained biological material (oral swabs) using real-time PCR. <i>Results:</i> One or more of the 12 tested HR-HPV genotypes were detected in 5/30 patients with OSCC and 2/30 with OPMD, whereas no healthy subjects were positive for any of the tested genotypes. There was a statistically significant difference in nodal involvement between HPV-positive and HPV-negative patients with OSCC. <i>Conclusions:</i> Oral HR-HPV was detected in patients with oral premalignant and malignant lesions but not in healthy individuals, suggesting a possible role in oral carcinogenesis. Broad HR-HPV panel testing could increase the sensitivity of risk assessment and screening for OSCC.https://www.mdpi.com/1648-9144/59/10/1843human papillomavirusoral squamous cell carcinomaoral potentially malignant disorders |
| spellingShingle | Anđelija Petrović Miloš Čanković Miloš Avramov Željko D. Popović Srđa Janković Slavko Mojsilović High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study Medicina human papillomavirus oral squamous cell carcinoma oral potentially malignant disorders |
| title | High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study |
| title_full | High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study |
| title_fullStr | High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study |
| title_full_unstemmed | High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study |
| title_short | High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study |
| title_sort | high risk human papillomavirus in patients with oral carcinoma and oral potentially malignant disorders in serbia a pilot study |
| topic | human papillomavirus oral squamous cell carcinoma oral potentially malignant disorders |
| url | https://www.mdpi.com/1648-9144/59/10/1843 |
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