Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproduction
<p>Abstract</p> <p>Background</p> <p>The pistil is a place where multiple interactions between cells of different types, origin, and function occur. Ca<sup>2+ </sup>is one of the key signal molecules in plants and animals. Despite the numerous studies on Ca&...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-11-01
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| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/11/150 |
| _version_ | 1818918672271409152 |
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| author | Zienkiewicz Krzysztof Rejón Juan D Suárez Cynthia Castro Antonio J de Dios Alché Juan Rodríguez García María |
| author_facet | Zienkiewicz Krzysztof Rejón Juan D Suárez Cynthia Castro Antonio J de Dios Alché Juan Rodríguez García María |
| author_sort | Zienkiewicz Krzysztof |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The pistil is a place where multiple interactions between cells of different types, origin, and function occur. Ca<sup>2+ </sup>is one of the key signal molecules in plants and animals. Despite the numerous studies on Ca<sup>2+ </sup>signalling during pollen-pistil interactions, which constitute one of the main topics of plant physiology, studies on Ca<sup>2+ </sup>dynamics in the pistil during flower formation are scarce. The purpose of this study was to analyze the contents and <it>in situ </it>localization of Ca<sup>2+ </sup>at the whole-organ level in the pistil of olive during the whole course of flower development.</p> <p>Results</p> <p>The obtained results showed significant changes in Ca<sup>2+ </sup>levels and distribution during olive pistil development. In the flower buds, the lowest levels of detectable Ca<sup>2+ </sup>were observed. As flower development proceeded, the Ca<sup>2+ </sup>amount in the pistil successively increased and reached the highest levels just after anther dehiscence. When the anthers and petals fell down a dramatic but not complete drop in calcium contents occurred in all pistil parts. <it>In situ </it>Ca<sup>2+ </sup>localization showed a gradual accumulation on the stigma, and further expansion toward the style and the ovary after anther dehiscence. At the post-anthesis phase, the Ca<sup>2+ </sup>signal on the stigmatic surface decreased, but in the ovary a specific accumulation of calcium was observed only in one of the four ovules. Ultrastructural localization confirmed the presence of Ca<sup>2+ </sup>in the intracellular matrix and in the exudate secreted by stigmatic papillae.</p> <p>Conclusions</p> <p>This is the first report to analyze calcium in the olive pistil during its development. According to our results <it>in situ </it>calcium localization by Fluo-3 AM injection is an effective tool to follow the pistil maturity degree and the spatial organization of calcium-dependent events of sexual reproduction occurring in developing pistil of angiosperms. The progressive increase of the Ca<sup>2+ </sup>pool during olive pistil development shown by us reflects the degree of pistil maturity. Ca<sup>2+ </sup>distribution at flower anthesis reflects the spatio-functional relationship of calcium with pollen-stigma interaction, progamic phase, fertilization and stigma senescence.</p> |
| first_indexed | 2024-12-20T00:53:41Z |
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| id | doaj.art-9c991e4026854b42b61b74b04f51518b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-20T00:53:41Z |
| publishDate | 2011-11-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-9c991e4026854b42b61b74b04f51518b2022-12-21T19:59:10ZengBMCBMC Plant Biology1471-22292011-11-0111115010.1186/1471-2229-11-150Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproductionZienkiewicz KrzysztofRejón Juan DSuárez CynthiaCastro Antonio Jde Dios Alché JuanRodríguez García María<p>Abstract</p> <p>Background</p> <p>The pistil is a place where multiple interactions between cells of different types, origin, and function occur. Ca<sup>2+ </sup>is one of the key signal molecules in plants and animals. Despite the numerous studies on Ca<sup>2+ </sup>signalling during pollen-pistil interactions, which constitute one of the main topics of plant physiology, studies on Ca<sup>2+ </sup>dynamics in the pistil during flower formation are scarce. The purpose of this study was to analyze the contents and <it>in situ </it>localization of Ca<sup>2+ </sup>at the whole-organ level in the pistil of olive during the whole course of flower development.</p> <p>Results</p> <p>The obtained results showed significant changes in Ca<sup>2+ </sup>levels and distribution during olive pistil development. In the flower buds, the lowest levels of detectable Ca<sup>2+ </sup>were observed. As flower development proceeded, the Ca<sup>2+ </sup>amount in the pistil successively increased and reached the highest levels just after anther dehiscence. When the anthers and petals fell down a dramatic but not complete drop in calcium contents occurred in all pistil parts. <it>In situ </it>Ca<sup>2+ </sup>localization showed a gradual accumulation on the stigma, and further expansion toward the style and the ovary after anther dehiscence. At the post-anthesis phase, the Ca<sup>2+ </sup>signal on the stigmatic surface decreased, but in the ovary a specific accumulation of calcium was observed only in one of the four ovules. Ultrastructural localization confirmed the presence of Ca<sup>2+ </sup>in the intracellular matrix and in the exudate secreted by stigmatic papillae.</p> <p>Conclusions</p> <p>This is the first report to analyze calcium in the olive pistil during its development. According to our results <it>in situ </it>calcium localization by Fluo-3 AM injection is an effective tool to follow the pistil maturity degree and the spatial organization of calcium-dependent events of sexual reproduction occurring in developing pistil of angiosperms. The progressive increase of the Ca<sup>2+ </sup>pool during olive pistil development shown by us reflects the degree of pistil maturity. Ca<sup>2+ </sup>distribution at flower anthesis reflects the spatio-functional relationship of calcium with pollen-stigma interaction, progamic phase, fertilization and stigma senescence.</p>http://www.biomedcentral.com/1471-2229/11/150 |
| spellingShingle | Zienkiewicz Krzysztof Rejón Juan D Suárez Cynthia Castro Antonio J de Dios Alché Juan Rodríguez García María Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproduction BMC Plant Biology |
| title | Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproduction |
| title_full | Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproduction |
| title_fullStr | Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproduction |
| title_full_unstemmed | Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproduction |
| title_short | Whole-Organ analysis of calcium behaviour in the developing pistil of olive (<it>Olea europaea </it>L.) as a tool for the determination of key events in sexual plant reproduction |
| title_sort | whole organ analysis of calcium behaviour in the developing pistil of olive it olea europaea it l as a tool for the determination of key events in sexual plant reproduction |
| url | http://www.biomedcentral.com/1471-2229/11/150 |
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