TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway
Abstract Background Tripartite motif‑containing 27 (TRIM27) belongs to the TRIM protein family, which is closely related to the progression of some certain human cancers. Nevertheless, the biological function of TRIM27 in esophageal squamous cell carcinoma (ESCC) is still not clear. The aim of prese...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12935-019-0998-4 |
| _version_ | 1819026154792681472 |
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| author | Liang Ma Ninghua Yao Ping Chen Zhixiang Zhuang |
| author_facet | Liang Ma Ninghua Yao Ping Chen Zhixiang Zhuang |
| author_sort | Liang Ma |
| collection | DOAJ |
| description | Abstract Background Tripartite motif‑containing 27 (TRIM27) belongs to the TRIM protein family, which is closely related to the progression of some certain human cancers. Nevertheless, the biological function of TRIM27 in esophageal squamous cell carcinoma (ESCC) is still not clear. The aim of present research is to examine the function of TRIM27 in ESCC cells. Methods In the present study, RNA interference (RNAi) and lentiviral vector were used to knockdown and overexpression of TRIM27 in ESCC cells respectively. qRT-PCR and western blot were used to examine the expression of TRIM27 in ESCC cells. Cell counting kit-8 (CCK-8) assay was performed to determine the proliferation of cells. Results Our analyses indicated that TRIM27 was a pro-proliferation factor in ESCC cells. Moreover, overexpression of TRIM27 deeply suppressed the apoptosis of ESCC cells and accelerated its glucose uptake. In addition, an AKT inhibitor LY294002 was used to determine the connection between TRIM27 and AKT in ESCC cells. Our results demonstrated that TRIM27 has involved in the PI3/AKT signaling pathway. Moreover, TRIM27 interacted with PTEN and mediated its poly-ubiquitination in ESCC cells. Importantly, the glycolysis inhibitor 3-BrPA also inhibited the effect of TRIM27 on ESCC cells. Hence, TRIM27 also participated in the regulation of energy metabolism in ESCC cells. Conclusions This research not only gained a deep insight into the biological function of TRIM27 but also elucidated its potential target and signaling pathway in human ESCC cells. |
| first_indexed | 2024-12-21T05:22:04Z |
| format | Article |
| id | doaj.art-9c9a876454f04c76836b63681e14b720 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-21T05:22:04Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-9c9a876454f04c76836b63681e14b7202022-12-21T19:14:48ZengBMCCancer Cell International1475-28672019-11-011911810.1186/s12935-019-0998-4TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathwayLiang Ma0Ninghua Yao1Ping Chen2Zhixiang Zhuang3Department of Oncology, The Second Affiliated Hospital of Soochow UniversityDepartments of Radiotherapy, Affiliated Hospital of Nantong UniversityDepartment of Oncology, First People’s Hospital of Yancheng, The Forth Affiliated Hospital of Nantong UniversityDepartment of Oncology, The Second Affiliated Hospital of Soochow UniversityAbstract Background Tripartite motif‑containing 27 (TRIM27) belongs to the TRIM protein family, which is closely related to the progression of some certain human cancers. Nevertheless, the biological function of TRIM27 in esophageal squamous cell carcinoma (ESCC) is still not clear. The aim of present research is to examine the function of TRIM27 in ESCC cells. Methods In the present study, RNA interference (RNAi) and lentiviral vector were used to knockdown and overexpression of TRIM27 in ESCC cells respectively. qRT-PCR and western blot were used to examine the expression of TRIM27 in ESCC cells. Cell counting kit-8 (CCK-8) assay was performed to determine the proliferation of cells. Results Our analyses indicated that TRIM27 was a pro-proliferation factor in ESCC cells. Moreover, overexpression of TRIM27 deeply suppressed the apoptosis of ESCC cells and accelerated its glucose uptake. In addition, an AKT inhibitor LY294002 was used to determine the connection between TRIM27 and AKT in ESCC cells. Our results demonstrated that TRIM27 has involved in the PI3/AKT signaling pathway. Moreover, TRIM27 interacted with PTEN and mediated its poly-ubiquitination in ESCC cells. Importantly, the glycolysis inhibitor 3-BrPA also inhibited the effect of TRIM27 on ESCC cells. Hence, TRIM27 also participated in the regulation of energy metabolism in ESCC cells. Conclusions This research not only gained a deep insight into the biological function of TRIM27 but also elucidated its potential target and signaling pathway in human ESCC cells.http://link.springer.com/article/10.1186/s12935-019-0998-4Esophagus cancerTRIM27PI3/AKTPTEN |
| spellingShingle | Liang Ma Ninghua Yao Ping Chen Zhixiang Zhuang TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway Cancer Cell International Esophagus cancer TRIM27 PI3/AKT PTEN |
| title | TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway |
| title_full | TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway |
| title_fullStr | TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway |
| title_full_unstemmed | TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway |
| title_short | TRIM27 promotes the development of esophagus cancer via regulating PTEN/AKT signaling pathway |
| title_sort | trim27 promotes the development of esophagus cancer via regulating pten akt signaling pathway |
| topic | Esophagus cancer TRIM27 PI3/AKT PTEN |
| url | http://link.springer.com/article/10.1186/s12935-019-0998-4 |
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