Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage
Tyrosol is a phenolic found in extra virgin olive oil (EVOO). In a Maltese monocultivar EVOO, it was present at a concentration of 9.23 ppm. The HL-60 acute myeloid leukaemia cell line, which can be differentiated to both monocytes and neutrophils, was exposed to tyrosol at this concentration and an...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2076-3417/11/21/10199 |
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| author | Lucienne Gatt David G. Saliba Pierre Schembri-Wismayer Marion Zammit-Mangion |
| author_facet | Lucienne Gatt David G. Saliba Pierre Schembri-Wismayer Marion Zammit-Mangion |
| author_sort | Lucienne Gatt |
| collection | DOAJ |
| description | Tyrosol is a phenolic found in extra virgin olive oil (EVOO). In a Maltese monocultivar EVOO, it was present at a concentration of 9.23 ppm. The HL-60 acute myeloid leukaemia cell line, which can be differentiated to both monocytes and neutrophils, was exposed to tyrosol at this concentration and analysed for evidence of differentiation and effects of cytotoxicity. The polyphenol induced a 1.93-fold increase in cellular oxidative activity (<i>p</i>-value 0.044) and enhanced surface expression of CD11b and CD14. This indicates that tyrosol induces monocytic-like differentiation. An RNA-seq analysis confirmed the upregulation of monocyte genes and the loss of neutrophil genes concomitant with the bi-potential promyelocyte precursor moving down the monocytic pathway. A cell cycle analysis showed an accumulation of cells in the Sub G<sub>0</sub>/G<sub>1</sub> phase following tyrosol exposure for 5 days, which coincided with an increase in apoptotic and necrotic markers. This indicates differentiation followed by cell death, unlike the positive monocyte differentiation control PMA. This selective cytotoxic effect following differentiation indicates therapeutic potential against leukaemia. |
| first_indexed | 2024-03-10T06:06:44Z |
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| id | doaj.art-9c9c5eaf81964ebf960bcd33eabe9a42 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T06:06:44Z |
| publishDate | 2021-10-01 |
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| spelling | doaj.art-9c9c5eaf81964ebf960bcd33eabe9a422023-11-22T20:29:16ZengMDPI AGApplied Sciences2076-34172021-10-0111211019910.3390/app112110199Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineageLucienne Gatt0David G. Saliba1Pierre Schembri-Wismayer2Marion Zammit-Mangion3Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, MSD 2080 Msida, MaltaCentre for Molecular Medicine and Biobanking, University of Malta, MSD 2080 Msida, MaltaDepartment of Anatomy, Faculty of Medicine and Surgery, University of Malta, MSD 2080 Msida, MaltaDepartment of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, MSD 2080 Msida, MaltaTyrosol is a phenolic found in extra virgin olive oil (EVOO). In a Maltese monocultivar EVOO, it was present at a concentration of 9.23 ppm. The HL-60 acute myeloid leukaemia cell line, which can be differentiated to both monocytes and neutrophils, was exposed to tyrosol at this concentration and analysed for evidence of differentiation and effects of cytotoxicity. The polyphenol induced a 1.93-fold increase in cellular oxidative activity (<i>p</i>-value 0.044) and enhanced surface expression of CD11b and CD14. This indicates that tyrosol induces monocytic-like differentiation. An RNA-seq analysis confirmed the upregulation of monocyte genes and the loss of neutrophil genes concomitant with the bi-potential promyelocyte precursor moving down the monocytic pathway. A cell cycle analysis showed an accumulation of cells in the Sub G<sub>0</sub>/G<sub>1</sub> phase following tyrosol exposure for 5 days, which coincided with an increase in apoptotic and necrotic markers. This indicates differentiation followed by cell death, unlike the positive monocyte differentiation control PMA. This selective cytotoxic effect following differentiation indicates therapeutic potential against leukaemia.https://www.mdpi.com/2076-3417/11/21/10199HL-60phenolsdifferentiationapoptosistranscriptome |
| spellingShingle | Lucienne Gatt David G. Saliba Pierre Schembri-Wismayer Marion Zammit-Mangion Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage Applied Sciences HL-60 phenols differentiation apoptosis transcriptome |
| title | Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage |
| title_full | Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage |
| title_fullStr | Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage |
| title_full_unstemmed | Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage |
| title_short | Tyrosol, at the Concentration Found in Maltese Extra Virgin Olive Oil, Induces HL-60 Differentiation towards the Monocyte lineage |
| title_sort | tyrosol at the concentration found in maltese extra virgin olive oil induces hl 60 differentiation towards the monocyte lineage |
| topic | HL-60 phenols differentiation apoptosis transcriptome |
| url | https://www.mdpi.com/2076-3417/11/21/10199 |
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