Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetes
Abstract Background Diabetes mellitus is a major cause of high mortality and poor prognosis in patients with pulmonary infections. However, limited data on the application of metagenomic next-generation sequencing (mNGS) are available for diabetic patients. This study aimed to evaluate the diagnosti...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | BMC Pulmonary Medicine |
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| Online Access: | https://doi.org/10.1186/s12890-023-02441-4 |
| _version_ | 1827956986932625408 |
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| author | Siqin Zhang Jing Ou Yuxue Tan Bin Yang Yaoyao Wu Lin Liu |
| author_facet | Siqin Zhang Jing Ou Yuxue Tan Bin Yang Yaoyao Wu Lin Liu |
| author_sort | Siqin Zhang |
| collection | DOAJ |
| description | Abstract Background Diabetes mellitus is a major cause of high mortality and poor prognosis in patients with pulmonary infections. However, limited data on the application of metagenomic next-generation sequencing (mNGS) are available for diabetic patients. This study aimed to evaluate the diagnostic performance of mNGS in diabetic patients with pulmonary infections. Methods We retrospectively reviewed 184 hospitalized patients with pulmonary infections at Guizhou Provincial People’s Hospital between January 2020 to October 2021. All patients were subjected to both mNGS analysis of bronchoalveolar lavage fluid (BALF) and conventional testing. Positive rate by mNGS and the consistency between mNGS and conventional testing results were evaluated for diabetic and non-diabetic patients. Results A total of 184 patients with pulmonary infections were enrolled, including 43 diabetic patients and 141 non-diabetic patients. For diabetic patients, the microbial positive rate by mNGS was significantly higher than that detected by conventional testing methods, primarily driven by bacterial detection (microbes: 95.3% vs. 67.4%, P = 0.001; bacteria: 72.1% vs. 37.2%, P = 0.001). mNGS and traditional tests had similar positive rates with regard to fungal and viral detection in diabetic patients. Klebsiella pneumoniae was the most common pathogen identified by mNGS in patients with diabetes. Moreover, mNGS identified pathogens in 92.9% (13/14) of diabetic patients who were reported negative by conventional testing. No significant difference was found in the consistency of the two tests between diabetic and non-diabetic groups. Conclusions mNGS is superior to conventional microbiological tests for bacterial detection in diabetic patients with pulmonary infections. mNGS is a valuable tool for etiological diagnosis of pulmonary infections in diabetic patients. |
| first_indexed | 2024-04-09T15:13:06Z |
| format | Article |
| id | doaj.art-9c9e73ec8fae424290c7447a8b932a6c |
| institution | Directory Open Access Journal |
| issn | 1471-2466 |
| language | English |
| last_indexed | 2024-04-09T15:13:06Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj.art-9c9e73ec8fae424290c7447a8b932a6c2023-04-30T11:06:15ZengBMCBMC Pulmonary Medicine1471-24662023-04-012311910.1186/s12890-023-02441-4Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetesSiqin Zhang0Jing Ou1Yuxue Tan2Bin Yang3Yaoyao Wu4Lin Liu5Department of Endocrinology and Metabolism, Guizhou Provincial People’s HospitalSchool of Medicine, Zunyi Medical UniversitySchool of Medicine, Zunyi Medical UniversityDepartment of Central Laboratory, Guizhou Provincial People’s HospitalDepartment of Respiratory and Critical Medicine, Guizhou Provincial People’s HospitalDepartment of Respiratory and Critical Medicine, Guizhou Provincial People’s HospitalAbstract Background Diabetes mellitus is a major cause of high mortality and poor prognosis in patients with pulmonary infections. However, limited data on the application of metagenomic next-generation sequencing (mNGS) are available for diabetic patients. This study aimed to evaluate the diagnostic performance of mNGS in diabetic patients with pulmonary infections. Methods We retrospectively reviewed 184 hospitalized patients with pulmonary infections at Guizhou Provincial People’s Hospital between January 2020 to October 2021. All patients were subjected to both mNGS analysis of bronchoalveolar lavage fluid (BALF) and conventional testing. Positive rate by mNGS and the consistency between mNGS and conventional testing results were evaluated for diabetic and non-diabetic patients. Results A total of 184 patients with pulmonary infections were enrolled, including 43 diabetic patients and 141 non-diabetic patients. For diabetic patients, the microbial positive rate by mNGS was significantly higher than that detected by conventional testing methods, primarily driven by bacterial detection (microbes: 95.3% vs. 67.4%, P = 0.001; bacteria: 72.1% vs. 37.2%, P = 0.001). mNGS and traditional tests had similar positive rates with regard to fungal and viral detection in diabetic patients. Klebsiella pneumoniae was the most common pathogen identified by mNGS in patients with diabetes. Moreover, mNGS identified pathogens in 92.9% (13/14) of diabetic patients who were reported negative by conventional testing. No significant difference was found in the consistency of the two tests between diabetic and non-diabetic groups. Conclusions mNGS is superior to conventional microbiological tests for bacterial detection in diabetic patients with pulmonary infections. mNGS is a valuable tool for etiological diagnosis of pulmonary infections in diabetic patients.https://doi.org/10.1186/s12890-023-02441-4Metagenomic next-generation sequencingPulmonary infectionDiabetesDiagnosis |
| spellingShingle | Siqin Zhang Jing Ou Yuxue Tan Bin Yang Yaoyao Wu Lin Liu Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetes BMC Pulmonary Medicine Metagenomic next-generation sequencing Pulmonary infection Diabetes Diagnosis |
| title | Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetes |
| title_full | Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetes |
| title_fullStr | Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetes |
| title_full_unstemmed | Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetes |
| title_short | Metagenomic next-generation sequencing for pulmonary infections diagnosis in patients with diabetes |
| title_sort | metagenomic next generation sequencing for pulmonary infections diagnosis in patients with diabetes |
| topic | Metagenomic next-generation sequencing Pulmonary infection Diabetes Diagnosis |
| url | https://doi.org/10.1186/s12890-023-02441-4 |
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