Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus”
Citrus Huanglongbing (HLB), caused by “Candidatus Liberibacter asiaticus” (CLas), is the most destructive disease threatening global citrus industry. Most commercial cultivars were susceptible to HLB, although some showed tolerant to HLB phenotypically. Identifying tolerant citrus genotypes and unde...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1191029/full |
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| author | Chenying Gao Chenying Gao Cuixiao Li Cuixiao Li Ziyi Li Ziyi Li Yaoxin Liu Yaoxin Liu Yaoxin Liu Jiaming Li Jiaming Li Jun Guo Jun Guo Jun Guo Jiana Mao Jiana Mao Fang Fang Fang Fang Cheng Wang Cheng Wang Xiaoling Deng Xiaoling Deng Zheng Zheng Zheng Zheng |
| author_facet | Chenying Gao Chenying Gao Cuixiao Li Cuixiao Li Ziyi Li Ziyi Li Yaoxin Liu Yaoxin Liu Yaoxin Liu Jiaming Li Jiaming Li Jun Guo Jun Guo Jun Guo Jiana Mao Jiana Mao Fang Fang Fang Fang Cheng Wang Cheng Wang Xiaoling Deng Xiaoling Deng Zheng Zheng Zheng Zheng |
| author_sort | Chenying Gao |
| collection | DOAJ |
| description | Citrus Huanglongbing (HLB), caused by “Candidatus Liberibacter asiaticus” (CLas), is the most destructive disease threatening global citrus industry. Most commercial cultivars were susceptible to HLB, although some showed tolerant to HLB phenotypically. Identifying tolerant citrus genotypes and understanding the mechanism correlated with tolerance to HLB is essential for breeding citrus variety tolerance/resistance to HLB. In this study, the graft assay with CLas-infected bud were performed in four citrus genotypes, including Citrus reticulata Blanco, C. sinensis, C. limon, and C. maxima. HLB tolerance was observed in C. limon and C. maxima, while C. Blanco and C. sinensis were susceptible to HLB. The time-course transcriptomic analysis revealed a significant variation in differentially expressed genes (DEGs) related to HLB between susceptible and tolerant cultivar group at early and late infection stage. Functional analysis of DEGs indicated that the activation of genes involved in SA-mediated defense response, PTI, cell wall associated immunity, endochitinase, phenylpropanoid and alpha-linolenic/linoleic lipid metabolism played an important in the tolerance of C. limon and C. maxima to HLB at early infection stage. In addition, the overactive plant defense combined with the stronger antibacterial activity (antibacterial secondary and lipid metabolism) and the suppression of pectinesterase were contributed to the long-term tolerance to HLB in C. limon and C. maxima at late infection stage. Particularly, the activation of ROS scavenging genes (catalases and ascorbate peroxidases) could help to reduce HLB symptoms in tolerant cultivars. In contrast, the overexpression of genes involved in oxidative burst and ethylene metabolism, as well as the late inducing of defense related genes could lead to the early HLB symptom development in susceptible cultivars at early infection stage. The weak defense response and antibacterial secondary metabolism, and the induce of pectinesterase were responsible for sensitivity to HLB in C. reticulata Blanco and C. sinensis at late infection stage. This study provided new insights into the tolerance/sensitivity mechanism against HLB and valuable guidance for breeding of HLB-tolerant/resistant cultivars. |
| first_indexed | 2024-03-13T05:37:42Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-13T05:37:42Z |
| publishDate | 2023-06-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-9c9f245acaf44b6d8f443061cbd79a552023-06-14T05:34:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-06-011410.3389/fpls.2023.11910291191029Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus”Chenying Gao0Chenying Gao1Cuixiao Li2Cuixiao Li3Ziyi Li4Ziyi Li5Yaoxin Liu6Yaoxin Liu7Yaoxin Liu8Jiaming Li9Jiaming Li10Jun Guo11Jun Guo12Jun Guo13Jiana Mao14Jiana Mao15Fang Fang16Fang Fang17Cheng Wang18Cheng Wang19Xiaoling Deng20Xiaoling Deng21Zheng Zheng22Zheng Zheng23National Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaHorticulture Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaInstitute of Tropical and Subtropical Cash Crops, Yunnan Academy of Agricultural Sciences, Baoshan, Yunnan, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaNational Key Laboratory of Green Pesticide, South China Agricultural University, Guangzhou, Guangdong, ChinaGuangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, ChinaCitrus Huanglongbing (HLB), caused by “Candidatus Liberibacter asiaticus” (CLas), is the most destructive disease threatening global citrus industry. Most commercial cultivars were susceptible to HLB, although some showed tolerant to HLB phenotypically. Identifying tolerant citrus genotypes and understanding the mechanism correlated with tolerance to HLB is essential for breeding citrus variety tolerance/resistance to HLB. In this study, the graft assay with CLas-infected bud were performed in four citrus genotypes, including Citrus reticulata Blanco, C. sinensis, C. limon, and C. maxima. HLB tolerance was observed in C. limon and C. maxima, while C. Blanco and C. sinensis were susceptible to HLB. The time-course transcriptomic analysis revealed a significant variation in differentially expressed genes (DEGs) related to HLB between susceptible and tolerant cultivar group at early and late infection stage. Functional analysis of DEGs indicated that the activation of genes involved in SA-mediated defense response, PTI, cell wall associated immunity, endochitinase, phenylpropanoid and alpha-linolenic/linoleic lipid metabolism played an important in the tolerance of C. limon and C. maxima to HLB at early infection stage. In addition, the overactive plant defense combined with the stronger antibacterial activity (antibacterial secondary and lipid metabolism) and the suppression of pectinesterase were contributed to the long-term tolerance to HLB in C. limon and C. maxima at late infection stage. Particularly, the activation of ROS scavenging genes (catalases and ascorbate peroxidases) could help to reduce HLB symptoms in tolerant cultivars. In contrast, the overexpression of genes involved in oxidative burst and ethylene metabolism, as well as the late inducing of defense related genes could lead to the early HLB symptom development in susceptible cultivars at early infection stage. The weak defense response and antibacterial secondary metabolism, and the induce of pectinesterase were responsible for sensitivity to HLB in C. reticulata Blanco and C. sinensis at late infection stage. This study provided new insights into the tolerance/sensitivity mechanism against HLB and valuable guidance for breeding of HLB-tolerant/resistant cultivars.https://www.frontiersin.org/articles/10.3389/fpls.2023.1191029/fullHuanglongbingCandidatus Liberibacter asiaticustolerancetranscriptomicshost response |
| spellingShingle | Chenying Gao Chenying Gao Cuixiao Li Cuixiao Li Ziyi Li Ziyi Li Yaoxin Liu Yaoxin Liu Yaoxin Liu Jiaming Li Jiaming Li Jun Guo Jun Guo Jun Guo Jiana Mao Jiana Mao Fang Fang Fang Fang Cheng Wang Cheng Wang Xiaoling Deng Xiaoling Deng Zheng Zheng Zheng Zheng Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus” Frontiers in Plant Science Huanglongbing Candidatus Liberibacter asiaticus tolerance transcriptomics host response |
| title | Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus” |
| title_full | Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus” |
| title_fullStr | Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus” |
| title_full_unstemmed | Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus” |
| title_short | Comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by “Candidatus Liberibacter asiaticus” |
| title_sort | comparative transcriptome profiling of susceptible and tolerant citrus species at early and late stage of infection by candidatus liberibacter asiaticus |
| topic | Huanglongbing Candidatus Liberibacter asiaticus tolerance transcriptomics host response |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1191029/full |
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