Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells
Background and Objective: In medical sciences, identifying the anticancer properties of plumbagin is of special importance. For this reason, this study investigated the anticancer activity of polymeric nanoparticles loaded by plumbagin against breast cancer cells. Methods: In this descriptive study,...
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| Format: | Article |
| Language: | fas |
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Golestan University of Medical Sciences
2022-07-01
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| Series: | مجله دانشگاه علوم پزشکی گرگان |
| Subjects: | |
| Online Access: | http://goums.ac.ir/journal/article-1-4012-en.html |
| _version_ | 1811237502505189376 |
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| author | Hossein Danafar Ali Sharafi Behrouz Parnianifar |
| author_facet | Hossein Danafar Ali Sharafi Behrouz Parnianifar |
| author_sort | Hossein Danafar |
| collection | DOAJ |
| description | Background and Objective: In medical sciences, identifying the anticancer properties of plumbagin is of special importance. For this reason, this study investigated the anticancer activity of polymeric nanoparticles loaded by plumbagin against breast cancer cells.
Methods: In this descriptive study, the diblock copolymer mPEG–PCL was synthesized by ring-opening polymerization of caprolactone in the presence of mPEG as the initiator and Sn(oct)2 as the catalyst. The synthesized copolymers were characterized by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance, gel permeation chromatography, and differential scanning calorimetry. The nanoprecipitation method was used for preparing nanoparticles loaded with plumbagin. The characteristics of these nanoparticles were investigated by various techniques including dynamic light scattering. The cytotoxicity of plumbagin, copolymer, and the nanoparticles loaded with plumbagin on MCF7 and HFF2 cells was evaluated by MTT assay.
Results: The average diameter of the nanoparticles was less than 115 nm. The loading capacity and encapsulation efficiencies were 15.4±0.13% and 79.1±0.65%, respectively. Drug release was slow, controlled, and almost dependent on pH. The results of the MTT assay showed strong and dose-dependent inhibition of cell growth by the plumbagin-loaded micelles compared with plumbagin alone in a way that the half maximal inhibitory concentration of this nanoparticle against MCF7 cells after 48 and 72 hours was 10.78 and 24.03 μM, respectively.
Conclusion: The mPEG-PCL nanoparticles can be an efficient carrier for plumbagin, and plumbagin can be an effective drug on breast cancer cells, without toxicity on healthy cells. |
| first_indexed | 2024-04-12T12:24:36Z |
| format | Article |
| id | doaj.art-9ca3787a42fb422fa642ac283ab52d27 |
| institution | Directory Open Access Journal |
| issn | 1562-4765 2008-4080 |
| language | fas |
| last_indexed | 2024-04-12T12:24:36Z |
| publishDate | 2022-07-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی گرگان |
| spelling | doaj.art-9ca3787a42fb422fa642ac283ab52d272022-12-22T03:33:13ZfasGolestan University of Medical Sciencesمجله دانشگاه علوم پزشکی گرگان1562-47652008-40802022-07-012428288Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cellsHossein Danafar0Ali Sharafi1Behrouz Parnianifar2 Associate Professor, Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran. Assistant Professor, Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran. Doctor of Pharmacy, Zanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran. Background and Objective: In medical sciences, identifying the anticancer properties of plumbagin is of special importance. For this reason, this study investigated the anticancer activity of polymeric nanoparticles loaded by plumbagin against breast cancer cells. Methods: In this descriptive study, the diblock copolymer mPEG–PCL was synthesized by ring-opening polymerization of caprolactone in the presence of mPEG as the initiator and Sn(oct)2 as the catalyst. The synthesized copolymers were characterized by Fourier-transform infrared spectroscopy, proton nuclear magnetic resonance, gel permeation chromatography, and differential scanning calorimetry. The nanoprecipitation method was used for preparing nanoparticles loaded with plumbagin. The characteristics of these nanoparticles were investigated by various techniques including dynamic light scattering. The cytotoxicity of plumbagin, copolymer, and the nanoparticles loaded with plumbagin on MCF7 and HFF2 cells was evaluated by MTT assay. Results: The average diameter of the nanoparticles was less than 115 nm. The loading capacity and encapsulation efficiencies were 15.4±0.13% and 79.1±0.65%, respectively. Drug release was slow, controlled, and almost dependent on pH. The results of the MTT assay showed strong and dose-dependent inhibition of cell growth by the plumbagin-loaded micelles compared with plumbagin alone in a way that the half maximal inhibitory concentration of this nanoparticle against MCF7 cells after 48 and 72 hours was 10.78 and 24.03 μM, respectively. Conclusion: The mPEG-PCL nanoparticles can be an efficient carrier for plumbagin, and plumbagin can be an effective drug on breast cancer cells, without toxicity on healthy cells.http://goums.ac.ir/journal/article-1-4012-en.htmlplumbagincytotoxicitynanoparticlesdiblock copolymers |
| spellingShingle | Hossein Danafar Ali Sharafi Behrouz Parnianifar Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells مجله دانشگاه علوم پزشکی گرگان plumbagin cytotoxicity nanoparticles diblock copolymers |
| title | Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells |
| title_full | Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells |
| title_fullStr | Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells |
| title_full_unstemmed | Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells |
| title_short | Anticancer Activity of Copolymeric Nanoparticles Loaded by Plumbagin on Breast Cancer cells |
| title_sort | anticancer activity of copolymeric nanoparticles loaded by plumbagin on breast cancer cells |
| topic | plumbagin cytotoxicity nanoparticles diblock copolymers |
| url | http://goums.ac.ir/journal/article-1-4012-en.html |
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