Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology
Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could...
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MDPI AG
2020-01-01
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author | Chen Zhu Xi Luo Wilfred Villariza Espulgar Shohei Koyama Atsushi Kumanogoh Masato Saito Hyota Takamatsu Eiichi Tamiya |
author_facet | Chen Zhu Xi Luo Wilfred Villariza Espulgar Shohei Koyama Atsushi Kumanogoh Masato Saito Hyota Takamatsu Eiichi Tamiya |
author_sort | Chen Zhu |
collection | DOAJ |
description | Cytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell. |
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format | Article |
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institution | Directory Open Access Journal |
issn | 2072-666X |
language | English |
last_indexed | 2024-04-12T23:11:57Z |
publishDate | 2020-01-01 |
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series | Micromachines |
spelling | doaj.art-9ca873134d204f889c80d932211bb45c2022-12-22T03:12:47ZengMDPI AGMicromachines2072-666X2020-01-0111110710.3390/mi11010107mi11010107Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR TechnologyChen Zhu0Xi Luo1Wilfred Villariza Espulgar2Shohei Koyama3Atsushi Kumanogoh4Masato Saito5Hyota Takamatsu6Eiichi Tamiya7Department of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanImmunology Frontier Research Center, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanImmunology Frontier Research Center, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanImmunology Frontier Research Center, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Applied Physics, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanCytokine secretion researches have been a main focus of studies among the scientists in the recent decades for its outstanding contribution to clinical diagnostics. Localized surface plasmon resonance (LSPR) technology is one of the conventional methods utilized to analyze these issues, as it could provide fast, label-free and real-time monitoring of biomolecule binding events. However, numerous LSPR-based biosensors in the past are usually utilized to monitor the average performance of cell groups rather than single cells. Meanwhile, the complicated sensor structures will lead to the fabrication and economic budget problems. Thus, in this paper, we report a simple synergistic integration of the cell trapping of microwell chip and gold-capped nanopillar-structured cyclo-olefin-polymer (COP) film for single cell level Interleukin 6 (IL-6) detection. Here, in-situ cytokine secreted from the trapped cell can be directly observed and analyzed through the peak red-shift in the transmittance spectrum. The fabricated device also shows the potential to conduct the real-time monitoring which would greatly help us identify the viability and biological variation of the tested single cell.https://www.mdpi.com/2072-666X/11/1/107localized surface plasmon resonance (lspr) technologyinterleukin 6 (il-6) detectionsingle cell trappingsingle cell level immunoassay |
spellingShingle | Chen Zhu Xi Luo Wilfred Villariza Espulgar Shohei Koyama Atsushi Kumanogoh Masato Saito Hyota Takamatsu Eiichi Tamiya Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology Micromachines localized surface plasmon resonance (lspr) technology interleukin 6 (il-6) detection single cell trapping single cell level immunoassay |
title | Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology |
title_full | Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology |
title_fullStr | Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology |
title_full_unstemmed | Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology |
title_short | Real-Time Monitoring and Detection of Single-Cell Level Cytokine Secretion Using LSPR Technology |
title_sort | real time monitoring and detection of single cell level cytokine secretion using lspr technology |
topic | localized surface plasmon resonance (lspr) technology interleukin 6 (il-6) detection single cell trapping single cell level immunoassay |
url | https://www.mdpi.com/2072-666X/11/1/107 |
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