First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic Animals
<i>Klebsiella pneumoniae</i> can cause serious pneumonitis in humans. The bacterium is also the common causative agent of hospital-acquired multidrug-resistant (MDR) infections. Here we for the first time reported the genetic diversity of <i>K. pneumoniae</i> strains in 14 sp...
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MDPI AG
2022-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/24/4058 |
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| author | Yingwei Xu Ling Ni Huiqiong Guan Dailing Chen Si Qin Lanming Chen |
| author_facet | Yingwei Xu Ling Ni Huiqiong Guan Dailing Chen Si Qin Lanming Chen |
| author_sort | Yingwei Xu |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> can cause serious pneumonitis in humans. The bacterium is also the common causative agent of hospital-acquired multidrug-resistant (MDR) infections. Here we for the first time reported the genetic diversity of <i>K. pneumoniae</i> strains in 14 species of edible aquatic animals sampled in the summer of 2018 and 2019 in Shanghai, China. Virulence-related genes were present in the <i>K. pneumoniae</i> strains (<i>n</i> = 94), including the <i>entB</i> (98.9%), <i>mrkD</i> (85.1%), <i>fimH</i> (50.0%), and <i>ybtA</i> (14.9%) strains. Resistance to sulfamethoxazole-trimethoprim was the most prevalent (52.1%), followed by chloramphenicol (31.9%), and tetracycline (27.7%), among the strains, wherein 34.0% had MDR phenotypes. Meanwhile, most strains were tolerant to heavy metals Cu<sup>2+</sup> (96.8%), Cr<sup>3+</sup> (96.8%), Zn<sup>2+</sup> (91.5%), Pb<sup>2+</sup> (89.4%), and Hg<sup>2+</sup> (81.9%). Remarkably, a higher abundance of the bacterium was found in bottom-dwelling aquatic animals, among which mollusk <i>Tegillarca granosa</i> contained <i>K. pneumoniae</i> 8-2-5-4 isolate from serotype K2 (ST-2026). Genome features of the potentially pathogenic isolate were characterized. The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR)–based genome fingerprinting classified the 94 <i>K. pneumoniae</i> strains into 76 ERIC genotypes with 63 singletons, demonstrating considerable genetic diversity in the strains. The findings of this study fill the gap in the risk assessment of <i>K. pneumoniae</i> in edible aquatic animals. |
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| language | English |
| last_indexed | 2024-03-09T16:41:19Z |
| publishDate | 2022-12-01 |
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| spelling | doaj.art-9caa9a7e574046269a5acd4206f0cc6d2023-11-24T14:51:41ZengMDPI AGFoods2304-81582022-12-011124405810.3390/foods11244058First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic AnimalsYingwei Xu0Ling Ni1Huiqiong Guan2Dailing Chen3Si Qin4Lanming Chen5Key Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, ChinaLab of Food Function and Nutrigenomics, College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, ChinaKey Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China<i>Klebsiella pneumoniae</i> can cause serious pneumonitis in humans. The bacterium is also the common causative agent of hospital-acquired multidrug-resistant (MDR) infections. Here we for the first time reported the genetic diversity of <i>K. pneumoniae</i> strains in 14 species of edible aquatic animals sampled in the summer of 2018 and 2019 in Shanghai, China. Virulence-related genes were present in the <i>K. pneumoniae</i> strains (<i>n</i> = 94), including the <i>entB</i> (98.9%), <i>mrkD</i> (85.1%), <i>fimH</i> (50.0%), and <i>ybtA</i> (14.9%) strains. Resistance to sulfamethoxazole-trimethoprim was the most prevalent (52.1%), followed by chloramphenicol (31.9%), and tetracycline (27.7%), among the strains, wherein 34.0% had MDR phenotypes. Meanwhile, most strains were tolerant to heavy metals Cu<sup>2+</sup> (96.8%), Cr<sup>3+</sup> (96.8%), Zn<sup>2+</sup> (91.5%), Pb<sup>2+</sup> (89.4%), and Hg<sup>2+</sup> (81.9%). Remarkably, a higher abundance of the bacterium was found in bottom-dwelling aquatic animals, among which mollusk <i>Tegillarca granosa</i> contained <i>K. pneumoniae</i> 8-2-5-4 isolate from serotype K2 (ST-2026). Genome features of the potentially pathogenic isolate were characterized. The enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR)–based genome fingerprinting classified the 94 <i>K. pneumoniae</i> strains into 76 ERIC genotypes with 63 singletons, demonstrating considerable genetic diversity in the strains. The findings of this study fill the gap in the risk assessment of <i>K. pneumoniae</i> in edible aquatic animals.https://www.mdpi.com/2304-8158/11/24/4058<i>Klebsiella pneumoniae</i>genetic diversityvirulenceantibiotic resistanceheavy metal toleranceaquatic animal |
| spellingShingle | Yingwei Xu Ling Ni Huiqiong Guan Dailing Chen Si Qin Lanming Chen First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic Animals Foods <i>Klebsiella pneumoniae</i> genetic diversity virulence antibiotic resistance heavy metal tolerance aquatic animal |
| title | First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic Animals |
| title_full | First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic Animals |
| title_fullStr | First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic Animals |
| title_full_unstemmed | First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic Animals |
| title_short | First Report of Potentially Pathogenic <i>Klebsiella pneumoniae</i> from Serotype K2 in Mollusk <i>Tegillarca granosa</i> and Genetic Diversity of <i>Klebsiella pneumoniae</i> in 14 Species of Edible Aquatic Animals |
| title_sort | first report of potentially pathogenic i klebsiella pneumoniae i from serotype k2 in mollusk i tegillarca granosa i and genetic diversity of i klebsiella pneumoniae i in 14 species of edible aquatic animals |
| topic | <i>Klebsiella pneumoniae</i> genetic diversity virulence antibiotic resistance heavy metal tolerance aquatic animal |
| url | https://www.mdpi.com/2304-8158/11/24/4058 |
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