In‐line monitoring of protein concentration with MIR spectroscopy during UFDF
Abstract Rapid increase of product titers in upstream processes has presented challenges for downstream processing, where purification costs increase linearly with the increase of the product yield. Hence, innovative solutions are becoming increasingly popular. Process Analytical Technology (PAT) to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-02-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202200050 |
| _version_ | 1828045089530707968 |
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| author | Adrianna Milewska Géraldine Baekelandt Sarra Boutaieb Vitalii Mozin Andrew Falconbridge |
| author_facet | Adrianna Milewska Géraldine Baekelandt Sarra Boutaieb Vitalii Mozin Andrew Falconbridge |
| author_sort | Adrianna Milewska |
| collection | DOAJ |
| description | Abstract Rapid increase of product titers in upstream processes has presented challenges for downstream processing, where purification costs increase linearly with the increase of the product yield. Hence, innovative solutions are becoming increasingly popular. Process Analytical Technology (PAT) tools, such as spectroscopic techniques, are on the rise due to their capacity to provide real‐time, precise analytics. This ensures consistent product quality and increased process understanding, as well as process control. Mid‐infrared spectroscopy (MIR) has emerged as a highly promising technique within recent years, owing to its ability to monitor several critical process parameters at the same time and unchallenging spectral analysis and data interpretation. For in‐line monitoring, Attenuated Total Reflectance—Fourier Transform Infrared Spectroscopy (ATR‐FTIR) is a method of choice, as it enables reliable measurements in a liquid environment, even though water absorption bands are present in the region of interest. Here, we present MIR spectroscopy as a monitoring tool of critical process parameters in ultrafiltration/diafiltration (UFDF). MIR spectrometer was integrated in the UFDF process in an in‐line fashion through a single‐use flow cell containing a single bounce silicon ATR crystal. The results indicate that the one‐point calibration algorithm applied to the MIR spectra, predicts highly accurate protein concentrations, as compared with validated offline analytical methods. |
| first_indexed | 2024-04-10T18:05:10Z |
| format | Article |
| id | doaj.art-9cb1109dac9c46208f9596649a3fb6a8 |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-04-10T18:05:10Z |
| publishDate | 2023-02-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-9cb1109dac9c46208f9596649a3fb6a82023-02-02T13:23:42ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632023-02-01232n/an/a10.1002/elsc.202200050In‐line monitoring of protein concentration with MIR spectroscopy during UFDFAdrianna Milewska0Géraldine Baekelandt1Sarra Boutaieb2Vitalii Mozin3Andrew Falconbridge4Alvotech Reykjavík IcelandIRUBIS GmbH München GermanyIRUBIS GmbH München GermanyIRUBIS GmbH München GermanyAlvotech Reykjavík IcelandAbstract Rapid increase of product titers in upstream processes has presented challenges for downstream processing, where purification costs increase linearly with the increase of the product yield. Hence, innovative solutions are becoming increasingly popular. Process Analytical Technology (PAT) tools, such as spectroscopic techniques, are on the rise due to their capacity to provide real‐time, precise analytics. This ensures consistent product quality and increased process understanding, as well as process control. Mid‐infrared spectroscopy (MIR) has emerged as a highly promising technique within recent years, owing to its ability to monitor several critical process parameters at the same time and unchallenging spectral analysis and data interpretation. For in‐line monitoring, Attenuated Total Reflectance—Fourier Transform Infrared Spectroscopy (ATR‐FTIR) is a method of choice, as it enables reliable measurements in a liquid environment, even though water absorption bands are present in the region of interest. Here, we present MIR spectroscopy as a monitoring tool of critical process parameters in ultrafiltration/diafiltration (UFDF). MIR spectrometer was integrated in the UFDF process in an in‐line fashion through a single‐use flow cell containing a single bounce silicon ATR crystal. The results indicate that the one‐point calibration algorithm applied to the MIR spectra, predicts highly accurate protein concentrations, as compared with validated offline analytical methods.https://doi.org/10.1002/elsc.202200050bioprocessingdownstream bioprocessingmid‐infrared spectroscopyprocess analytical technology |
| spellingShingle | Adrianna Milewska Géraldine Baekelandt Sarra Boutaieb Vitalii Mozin Andrew Falconbridge In‐line monitoring of protein concentration with MIR spectroscopy during UFDF Engineering in Life Sciences bioprocessing downstream bioprocessing mid‐infrared spectroscopy process analytical technology |
| title | In‐line monitoring of protein concentration with MIR spectroscopy during UFDF |
| title_full | In‐line monitoring of protein concentration with MIR spectroscopy during UFDF |
| title_fullStr | In‐line monitoring of protein concentration with MIR spectroscopy during UFDF |
| title_full_unstemmed | In‐line monitoring of protein concentration with MIR spectroscopy during UFDF |
| title_short | In‐line monitoring of protein concentration with MIR spectroscopy during UFDF |
| title_sort | in line monitoring of protein concentration with mir spectroscopy during ufdf |
| topic | bioprocessing downstream bioprocessing mid‐infrared spectroscopy process analytical technology |
| url | https://doi.org/10.1002/elsc.202200050 |
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