Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics
Bacterial adhesion to dental implants is the onset for the development of pathological biofilms. Reliable characterization of this initial process is the basis towards the development of anti-biofilm strategies. In the present study, single-cell force spectroscopy (SCFS), by means of an atomic force...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2306-5354/9/10/567 |
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| author | Katharina Doll-Nikutta Andreas Winkel Ines Yang Anna Josefine Grote Nils Meier Mosaieb Habib Henning Menzel Peter Behrens Meike Stiesch |
| author_facet | Katharina Doll-Nikutta Andreas Winkel Ines Yang Anna Josefine Grote Nils Meier Mosaieb Habib Henning Menzel Peter Behrens Meike Stiesch |
| author_sort | Katharina Doll-Nikutta |
| collection | DOAJ |
| description | Bacterial adhesion to dental implants is the onset for the development of pathological biofilms. Reliable characterization of this initial process is the basis towards the development of anti-biofilm strategies. In the present study, single-cell force spectroscopy (SCFS), by means of an atomic force microscope connected to a microfluidic pressure control system (FluidFM), was used to comparably measure adhesion forces of different oral bacteria within a similar experimental setup to the common implant material titanium. The bacteria selected belong to different ecological niches in oral biofilms: the commensal pioneers <i>Streptococcus oralis</i> and <i>Actinomyces naeslundii</i>; secondary colonizer <i>Veillonella dispar</i>; and the late colonizing pathogens <i>Porphyromonas gingivalis</i> as well as fimbriated and non-fimbriated <i>Aggregatibacter actinomycetemcomitans</i>. The results showed highest values for early colonizing pioneer species, strengthening the link between adhesion forces and bacteria’s role in oral biofilm development. Additionally, the correlation between biophysical cellular characteristics and SCFS results across species was analyzed. Here, distinct correlations between electrostatically driven maximum adhesion force, bacterial surface elasticity and surface charge as well as single-molecule attachment points, stretching capability and metabolic activity, could be identified. Therefore, this study provides a step towards the detailed understanding of oral bacteria initial adhesion and could support the development of infection-resistant implant materials in future. |
| first_indexed | 2024-03-09T20:40:59Z |
| format | Article |
| id | doaj.art-9cb1757f20c54a41ab3e5372ce6f4a18 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T20:40:59Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-9cb1757f20c54a41ab3e5372ce6f4a182023-11-23T22:57:58ZengMDPI AGBioengineering2306-53542022-10-0191056710.3390/bioengineering9100567Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular CharacteristicsKatharina Doll-Nikutta0Andreas Winkel1Ines Yang2Anna Josefine Grote3Nils Meier4Mosaieb Habib5Henning Menzel6Peter Behrens7Meike Stiesch8Department of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, GermanyInstitute for Technical Chemistry, Technische Universität Braunschweig, Hagenring 30, 38106 Braunschweig, GermanyLower Saxony Centre for Biomedical Engineering, Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, GermanyInstitute for Technical Chemistry, Technische Universität Braunschweig, Hagenring 30, 38106 Braunschweig, GermanyLower Saxony Centre for Biomedical Engineering, Implant Research and Development (NIFE), Stadtfelddamm 34, 30625 Hannover, GermanyDepartment of Prosthetic Dentistry and Biomedical Materials Science, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, GermanyBacterial adhesion to dental implants is the onset for the development of pathological biofilms. Reliable characterization of this initial process is the basis towards the development of anti-biofilm strategies. In the present study, single-cell force spectroscopy (SCFS), by means of an atomic force microscope connected to a microfluidic pressure control system (FluidFM), was used to comparably measure adhesion forces of different oral bacteria within a similar experimental setup to the common implant material titanium. The bacteria selected belong to different ecological niches in oral biofilms: the commensal pioneers <i>Streptococcus oralis</i> and <i>Actinomyces naeslundii</i>; secondary colonizer <i>Veillonella dispar</i>; and the late colonizing pathogens <i>Porphyromonas gingivalis</i> as well as fimbriated and non-fimbriated <i>Aggregatibacter actinomycetemcomitans</i>. The results showed highest values for early colonizing pioneer species, strengthening the link between adhesion forces and bacteria’s role in oral biofilm development. Additionally, the correlation between biophysical cellular characteristics and SCFS results across species was analyzed. Here, distinct correlations between electrostatically driven maximum adhesion force, bacterial surface elasticity and surface charge as well as single-molecule attachment points, stretching capability and metabolic activity, could be identified. Therefore, this study provides a step towards the detailed understanding of oral bacteria initial adhesion and could support the development of infection-resistant implant materials in future.https://www.mdpi.com/2306-5354/9/10/567atomic force microscopysingle-cell spectroscopybacterial adhesioncell surfacecell respirationdental implant |
| spellingShingle | Katharina Doll-Nikutta Andreas Winkel Ines Yang Anna Josefine Grote Nils Meier Mosaieb Habib Henning Menzel Peter Behrens Meike Stiesch Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics Bioengineering atomic force microscopy single-cell spectroscopy bacterial adhesion cell surface cell respiration dental implant |
| title | Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics |
| title_full | Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics |
| title_fullStr | Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics |
| title_full_unstemmed | Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics |
| title_short | Adhesion Forces of Oral Bacteria to Titanium and the Correlation with Biophysical Cellular Characteristics |
| title_sort | adhesion forces of oral bacteria to titanium and the correlation with biophysical cellular characteristics |
| topic | atomic force microscopy single-cell spectroscopy bacterial adhesion cell surface cell respiration dental implant |
| url | https://www.mdpi.com/2306-5354/9/10/567 |
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