Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term Study
(1) Background: Vaccination of dogs against leptospirosis is of paramount importance, as they ideally must provide not only long-term protection, but also against the renal carrier state of leptospires. This study assessed the post-vaccine humoral response against <i>Leptospira</i> in na...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/12/14/1788 |
| _version_ | 1797433963271159808 |
|---|---|
| author | Ricardo Sant’Anna da Costa Maria Isabel N. Di Azevedo Ana Luiza dos Santos Baptista Borges Luíza Aymée Gabriel Martins Walter Lilenbaum |
| author_facet | Ricardo Sant’Anna da Costa Maria Isabel N. Di Azevedo Ana Luiza dos Santos Baptista Borges Luíza Aymée Gabriel Martins Walter Lilenbaum |
| author_sort | Ricardo Sant’Anna da Costa |
| collection | DOAJ |
| description | (1) Background: Vaccination of dogs against leptospirosis is of paramount importance, as they ideally must provide not only long-term protection, but also against the renal carrier state of leptospires. This study assessed the post-vaccine humoral response against <i>Leptospira</i> in naturally exposed dogs and effects on renal carrier status. (2) Methods: A total of 118 dogs were studied for 365 days, separated into Group A (vaccinated, <i>n</i> = 94) and Group B (non-vaccinated, <i>n</i> = 24). Group A was subdivided into three groups: A1 with 32 dogs immunized with the vaccine #1; A2 by 32 dogs with #2; and A3 30 dogs with #3. Serology (MAT and IgG-ELISA) and urinary PCR were conducted. (3) Results: Seroreactivity increased at D15 post-vaccination and, regardless of vaccine brand, remained high up to D180, with antibody switch to IgG after D30. A total of 46.8% of animals from Group A were PCR-positive at least once, in contrast to 75% in Group B, regardless of vaccine brand (<i>p</i> < 0.05; OR: 0.3). (4) Conclusions: All commercial vaccines succeeded at eliciting a long-term IgG-based response and were partially effective at protecting against kidney infection. |
| first_indexed | 2024-03-09T10:24:27Z |
| format | Article |
| id | doaj.art-9cb21aec0945432294d88551d373e711 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T10:24:27Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-9cb21aec0945432294d88551d373e7112023-12-01T21:48:23ZengMDPI AGAnimals2076-26152022-07-011214178810.3390/ani12141788Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term StudyRicardo Sant’Anna da Costa0Maria Isabel N. Di Azevedo1Ana Luiza dos Santos Baptista Borges2Luíza Aymée3Gabriel Martins4Walter Lilenbaum5Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói 24210-130, BrazilLaboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói 24210-130, BrazilLaboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói 24210-130, BrazilLaboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói 24210-130, BrazilCentro Universitário Arthur Sá Earp Neto, Petrópolis 25680-120, BrazilLaboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói 24210-130, Brazil(1) Background: Vaccination of dogs against leptospirosis is of paramount importance, as they ideally must provide not only long-term protection, but also against the renal carrier state of leptospires. This study assessed the post-vaccine humoral response against <i>Leptospira</i> in naturally exposed dogs and effects on renal carrier status. (2) Methods: A total of 118 dogs were studied for 365 days, separated into Group A (vaccinated, <i>n</i> = 94) and Group B (non-vaccinated, <i>n</i> = 24). Group A was subdivided into three groups: A1 with 32 dogs immunized with the vaccine #1; A2 by 32 dogs with #2; and A3 30 dogs with #3. Serology (MAT and IgG-ELISA) and urinary PCR were conducted. (3) Results: Seroreactivity increased at D15 post-vaccination and, regardless of vaccine brand, remained high up to D180, with antibody switch to IgG after D30. A total of 46.8% of animals from Group A were PCR-positive at least once, in contrast to 75% in Group B, regardless of vaccine brand (<i>p</i> < 0.05; OR: 0.3). (4) Conclusions: All commercial vaccines succeeded at eliciting a long-term IgG-based response and were partially effective at protecting against kidney infection.https://www.mdpi.com/2076-2615/12/14/1788canine leptospirosissequencingcommercial vaccinesilent infectionleptospiral infectionsubclinical infection |
| spellingShingle | Ricardo Sant’Anna da Costa Maria Isabel N. Di Azevedo Ana Luiza dos Santos Baptista Borges Luíza Aymée Gabriel Martins Walter Lilenbaum Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term Study Animals canine leptospirosis sequencing commercial vaccine silent infection leptospiral infection subclinical infection |
| title | Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term Study |
| title_full | Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term Study |
| title_fullStr | Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term Study |
| title_full_unstemmed | Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term Study |
| title_short | Effect of Vaccination against <i>Leptospira</i> on Shelter Asymptomatic Dogs Following a Long-Term Study |
| title_sort | effect of vaccination against i leptospira i on shelter asymptomatic dogs following a long term study |
| topic | canine leptospirosis sequencing commercial vaccine silent infection leptospiral infection subclinical infection |
| url | https://www.mdpi.com/2076-2615/12/14/1788 |
| work_keys_str_mv | AT ricardosantannadacosta effectofvaccinationagainstileptospiraionshelterasymptomaticdogsfollowingalongtermstudy AT mariaisabelndiazevedo effectofvaccinationagainstileptospiraionshelterasymptomaticdogsfollowingalongtermstudy AT analuizadossantosbaptistaborges effectofvaccinationagainstileptospiraionshelterasymptomaticdogsfollowingalongtermstudy AT luizaaymee effectofvaccinationagainstileptospiraionshelterasymptomaticdogsfollowingalongtermstudy AT gabrielmartins effectofvaccinationagainstileptospiraionshelterasymptomaticdogsfollowingalongtermstudy AT walterlilenbaum effectofvaccinationagainstileptospiraionshelterasymptomaticdogsfollowingalongtermstudy |