Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay
Summary: Peptide mobility shift assays provide a sensitive measure of kinase enzymatic activity and can be used to evaluate kinase inhibitors. Herein, we describe a protocol adapted for rapid assessment of doublecortin-like kinase inhibitors. Advantages include rapid iterations of therapeutic compou...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672100294X |
| _version_ | 1829502314314989568 |
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| author | Yan Liu Kenneth D. Westover |
| author_facet | Yan Liu Kenneth D. Westover |
| author_sort | Yan Liu |
| collection | DOAJ |
| description | Summary: Peptide mobility shift assays provide a sensitive measure of kinase enzymatic activity and can be used to evaluate kinase inhibitors. Herein, we describe a protocol adapted for rapid assessment of doublecortin-like kinase inhibitors. Advantages include rapid iterations of therapeutic compound assessment and the ability to characterize kinase mutations, such as drug-resistant mutants for biological rescue experiments, on kinase activity.For complete details on the use and execution of this protocol, please refer to Liu et al. (2020). |
| first_indexed | 2024-12-16T09:36:00Z |
| format | Article |
| id | doaj.art-9cb2921000cf45d5adecab4106b3f7fa |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-16T09:36:00Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-9cb2921000cf45d5adecab4106b3f7fa2022-12-21T22:36:25ZengElsevierSTAR Protocols2666-16672021-06-0122100587Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assayYan Liu0Kenneth D. Westover1Department of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA; Department of Radiation Oncology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA; Corresponding authorDepartment of Biochemistry, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA; Department of Radiation Oncology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA; Corresponding authorSummary: Peptide mobility shift assays provide a sensitive measure of kinase enzymatic activity and can be used to evaluate kinase inhibitors. Herein, we describe a protocol adapted for rapid assessment of doublecortin-like kinase inhibitors. Advantages include rapid iterations of therapeutic compound assessment and the ability to characterize kinase mutations, such as drug-resistant mutants for biological rescue experiments, on kinase activity.For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).http://www.sciencedirect.com/science/article/pii/S266616672100294Xcancerhigh-throughput screeningmolecular/chemical probesprotein biochemistry |
| spellingShingle | Yan Liu Kenneth D. Westover Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay STAR Protocols cancer high-throughput screening molecular/chemical probes protein biochemistry |
| title | Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay |
| title_full | Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay |
| title_fullStr | Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay |
| title_full_unstemmed | Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay |
| title_short | Rapid assessment of DCLK1 inhibitors using a peptide substrate mobility shift assay |
| title_sort | rapid assessment of dclk1 inhibitors using a peptide substrate mobility shift assay |
| topic | cancer high-throughput screening molecular/chemical probes protein biochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S266616672100294X |
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