Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
Biocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of conc...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-08-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/10/8/1575 |
| _version_ | 1797558889887039488 |
|---|---|
| author | Karthiga K. Anpalagan Jimsheena V. Karakkat Adam Truskewycz Ahmed Al Saedi Paul Joseph Vasso Apostolopoulos Kulmira Nurgali Ivan Cole Zibo Cai Daniel T. H. Lai |
| author_facet | Karthiga K. Anpalagan Jimsheena V. Karakkat Adam Truskewycz Ahmed Al Saedi Paul Joseph Vasso Apostolopoulos Kulmira Nurgali Ivan Cole Zibo Cai Daniel T. H. Lai |
| author_sort | Karthiga K. Anpalagan |
| collection | DOAJ |
| description | Biocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of concentrated mineral acids and other corrosive chemicals during the fabrication process curtails their biocompatibility and severely limits the utilization of the products in cell bio-imaging. In this study; a facile; fast; and cost-effective synthetic route is employed to fabricate CQDs from a natural organic resource; namely bread; where the use of any toxic chemicals is eliminated. Thus; the novel chemical-free technique facilitated the production of luminescent CQDs that were endowed with low cytotoxicity and; therefore; suitable candidates for bioimaging sensors. The above mentioned amorphous CQDs also exhibited fluorescence over 360–420 nm excitation wavelengths; and with a broad emission range of 360–600 nm. We have also shown that the CQDs were well internalized by muscle myoblasts (C2C12) and differentiated myotubes; the cell lines which have not been reported before. |
| first_indexed | 2024-03-10T17:37:44Z |
| format | Article |
| id | doaj.art-9cb4bb9d1e6745f88bc253e2172ebcb2 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T17:37:44Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-9cb4bb9d1e6745f88bc253e2172ebcb22023-11-20T09:48:19ZengMDPI AGNanomaterials2079-49912020-08-01108157510.3390/nano10081575Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from BreadKarthiga K. Anpalagan0Jimsheena V. Karakkat1Adam Truskewycz2Ahmed Al Saedi3Paul Joseph4Vasso Apostolopoulos5Kulmira Nurgali6Ivan Cole7Zibo Cai8Daniel T. H. Lai9Institute of Health and Sport (IHES), Victoria University, Melbourne, VIC 3011, AustraliaInstitute of Health and Sport (IHES), Victoria University, Melbourne, VIC 3011, AustraliaAdvanced Manufacturing and Fabrication, School of Engineering, Royal Melbourne Institute of Technology (RMIT), Melbourne, VIC 3000, AustraliaAustralian Institute for Musculoskeletal Science (AIMSS), The University of Melbourne and Western Health, St. Albans, VIC 3000, AustraliaInstitute of Sustainable Industries and Liveable Cities, Victoria University, Melbourne, VIC 3011, AustraliaInstitute of Health and Sport (IHES), Victoria University, Melbourne, VIC 3011, AustraliaInstitute of Health and Sport (IHES), Victoria University, Melbourne, VIC 3011, AustraliaAdvanced Manufacturing and Fabrication, School of Engineering, Royal Melbourne Institute of Technology (RMIT), Melbourne, VIC 3000, AustraliaInstitute of Health and Sport (IHES), Victoria University, Melbourne, VIC 3011, AustraliaInstitute of Health and Sport (IHES), Victoria University, Melbourne, VIC 3011, AustraliaBiocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of concentrated mineral acids and other corrosive chemicals during the fabrication process curtails their biocompatibility and severely limits the utilization of the products in cell bio-imaging. In this study; a facile; fast; and cost-effective synthetic route is employed to fabricate CQDs from a natural organic resource; namely bread; where the use of any toxic chemicals is eliminated. Thus; the novel chemical-free technique facilitated the production of luminescent CQDs that were endowed with low cytotoxicity and; therefore; suitable candidates for bioimaging sensors. The above mentioned amorphous CQDs also exhibited fluorescence over 360–420 nm excitation wavelengths; and with a broad emission range of 360–600 nm. We have also shown that the CQDs were well internalized by muscle myoblasts (C2C12) and differentiated myotubes; the cell lines which have not been reported before.https://www.mdpi.com/2079-4991/10/8/1575carbon quantum dotsgreen synthesisbioimagingC2C12 muscle myoblast |
| spellingShingle | Karthiga K. Anpalagan Jimsheena V. Karakkat Adam Truskewycz Ahmed Al Saedi Paul Joseph Vasso Apostolopoulos Kulmira Nurgali Ivan Cole Zibo Cai Daniel T. H. Lai Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread Nanomaterials carbon quantum dots green synthesis bioimaging C2C12 muscle myoblast |
| title | Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread |
| title_full | Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread |
| title_fullStr | Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread |
| title_full_unstemmed | Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread |
| title_short | Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread |
| title_sort | bioimaging of c2c12 muscle myoblasts using fluorescent carbon quantum dots synthesized from bread |
| topic | carbon quantum dots green synthesis bioimaging C2C12 muscle myoblast |
| url | https://www.mdpi.com/2079-4991/10/8/1575 |
| work_keys_str_mv | AT karthigakanpalagan bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT jimsheenavkarakkat bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT adamtruskewycz bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT ahmedalsaedi bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT pauljoseph bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT vassoapostolopoulos bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT kulmiranurgali bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT ivancole bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT zibocai bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread AT danielthlai bioimagingofc2c12musclemyoblastsusingfluorescentcarbonquantumdotssynthesizedfrombread |